Mycobacterium phage Kazan

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143FQ95|A0A143FQ95_9CAUD Uncharacterized protein OS=Mycobacterium phage Kazan OX=1821726 GN=89 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.24PVKK6 pKa = 10.33TFVEE10 pKa = 4.66VWGGSHH16 pKa = 7.28LAADD20 pKa = 3.92IADD23 pKa = 3.81KK24 pKa = 9.59LTCNEE29 pKa = 4.01AEE31 pKa = 4.73AIASLFLGLGDD42 pKa = 4.57VEE44 pKa = 5.86SADD47 pKa = 3.8FWIEE51 pKa = 3.54HH52 pKa = 6.5HH53 pKa = 7.17ADD55 pKa = 4.6SDD57 pKa = 4.29DD58 pKa = 5.27CDD60 pKa = 5.22DD61 pKa = 3.84DD62 pKa = 3.86HH63 pKa = 7.41CRR65 pKa = 11.84CEE67 pKa = 3.93DD68 pKa = 3.57CKK70 pKa = 11.44EE71 pKa = 4.0NGG73 pKa = 3.33

Molecular weight: 7.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143FR23|A0A143FR23_9CAUD AAA domain-containing protein OS=Mycobacterium phage Kazan OX=1821726 GN=71 PE=4 SV=1
MM1 pKa = 6.94TWAGSRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84DD10 pKa = 3.66EE11 pKa = 5.73LPPDD15 pKa = 3.31WEE17 pKa = 4.17LKK19 pKa = 9.99YY20 pKa = 10.54RR21 pKa = 11.84LPVLSAANWLCEE33 pKa = 4.13VNGPGCVRR41 pKa = 11.84AATDD45 pKa = 3.09VDD47 pKa = 3.71HH48 pKa = 7.41KK49 pKa = 11.35KK50 pKa = 10.48RR51 pKa = 11.84GNDD54 pKa = 3.22HH55 pKa = 6.52SRR57 pKa = 11.84SNLQAICRR65 pKa = 11.84VCHH68 pKa = 5.35GRR70 pKa = 11.84KK71 pKa = 8.97SAAEE75 pKa = 3.85GVARR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84EE82 pKa = 3.88LKK84 pKa = 10.28ARR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 9.81RR89 pKa = 11.84PPQRR93 pKa = 11.84HH94 pKa = 6.14PGQLL98 pKa = 3.22

Molecular weight: 11.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

16255

26

734

160.9

17.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.345 ± 0.276

0.96 ± 0.125

6.466 ± 0.177

6.841 ± 0.296

3.457 ± 0.182

8.853 ± 0.617

1.95 ± 0.159

5.088 ± 0.179

4.989 ± 0.236

7.844 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.128

3.427 ± 0.181

5.555 ± 0.256

3.673 ± 0.223

6.023 ± 0.297

5.623 ± 0.27

5.789 ± 0.184

6.902 ± 0.26

2.086 ± 0.126

2.855 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski