Tamlana carrageenivorans
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3310 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I7SDN7|A0A2I7SDN7_9FLAO Transcriptional regulator OS=Tamlana carrageenivorans OX=2069432 GN=C1A40_00250 PE=4 SV=1
MM1 pKa = 7.74 KK2 pKa = 10.48 LMKK5 pKa = 10.57 LSLFVLGLATCFLSCKK21 pKa = 10.13 KK22 pKa = 10.65 DD23 pKa = 3.64 DD24 pKa = 5.25 DD25 pKa = 5.42 DD26 pKa = 6.83 DD27 pKa = 4.03 GTTIEE32 pKa = 4.0 VRR34 pKa = 11.84 DD35 pKa = 3.59 RR36 pKa = 11.84 AEE38 pKa = 3.59 QQIEE42 pKa = 4.32 DD43 pKa = 3.81 KK44 pKa = 11.28 ALLLDD49 pKa = 3.87 YY50 pKa = 11.41 LEE52 pKa = 4.12 THH54 pKa = 6.86 YY55 pKa = 11.61 YY56 pKa = 8.72 NASDD60 pKa = 4.08 FDD62 pKa = 4.32 GSNPDD67 pKa = 3.8 PKK69 pKa = 11.01 SIDD72 pKa = 3.35 IEE74 pKa = 4.02 ITEE77 pKa = 4.25 LKK79 pKa = 10.57 EE80 pKa = 3.99 NEE82 pKa = 4.4 TVPTGHH88 pKa = 7.54 RR89 pKa = 11.84 LLIQDD94 pKa = 4.79 TIMKK98 pKa = 9.65 YY99 pKa = 7.11 VTYY102 pKa = 10.8 YY103 pKa = 10.18 DD104 pKa = 3.57 VKK106 pKa = 10.99 YY107 pKa = 10.21 EE108 pKa = 4.39 IYY110 pKa = 11.0 YY111 pKa = 10.7 LDD113 pKa = 3.92 LNKK116 pKa = 10.49 NVDD119 pKa = 3.24 KK120 pKa = 10.08 TAPSPHH126 pKa = 6.17 FCDD129 pKa = 4.46 NVVTNYY135 pKa = 10.76 KK136 pKa = 10.72 GFTLDD141 pKa = 3.67 NEE143 pKa = 4.37 VFDD146 pKa = 4.98 RR147 pKa = 11.84 SEE149 pKa = 3.83 TGAFNSDD156 pKa = 3.5 LLNNIDD162 pKa = 3.06 AWQIVIPEE170 pKa = 4.94 FYY172 pKa = 9.55 TAEE175 pKa = 3.61 AFMEE179 pKa = 4.58 NNDD182 pKa = 3.4 GTSNYY187 pKa = 8.62 INHH190 pKa = 6.63 GVGVMFAPSGLGYY203 pKa = 10.28 FSSVRR208 pKa = 11.84 PGIPSYY214 pKa = 11.38 SCLVFNFDD222 pKa = 3.51 VLQMFEE228 pKa = 4.52 NDD230 pKa = 3.19 HH231 pKa = 7.23 DD232 pKa = 5.03 GDD234 pKa = 4.21 GVPSYY239 pKa = 11.23 KK240 pKa = 10.25 EE241 pKa = 3.98 DD242 pKa = 3.76 LNGDD246 pKa = 3.61 GKK248 pKa = 9.85 FTYY251 pKa = 10.5 NSAEE255 pKa = 4.08 GVFDD259 pKa = 5.95 GDD261 pKa = 3.73 DD262 pKa = 3.43 TDD264 pKa = 5.21 ANGILDD270 pKa = 5.12 FVDD273 pKa = 3.68 TDD275 pKa = 3.97 DD276 pKa = 6.28 DD277 pKa = 5.09 GDD279 pKa = 4.17 GVLTIYY285 pKa = 10.96 EE286 pKa = 5.12 DD287 pKa = 3.44 IDD289 pKa = 4.7 GDD291 pKa = 4.12 GDD293 pKa = 3.69 PTNDD297 pKa = 3.06 IGKK300 pKa = 9.62 NGIPKK305 pKa = 10.24 YY306 pKa = 10.35 LDD308 pKa = 3.66 PEE310 pKa = 4.19 EE311 pKa = 4.78 TEE313 pKa = 4.02 ANYY316 pKa = 10.62 DD317 pKa = 3.16 II318 pKa = 5.62
Molecular weight: 35.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.605
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.516
Solomon 3.834
Lehninger 3.783
Nozaki 3.935
DTASelect 4.215
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.961
Patrickios 1.392
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.847
Protein with the highest isoelectric point:
>tr|A0A2I7SE80|A0A2I7SE80_9FLAO 50S ribosomal protein L31 OS=Tamlana carrageenivorans OX=2069432 GN=C1A40_01300 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 7.97 KK42 pKa = 10.72 LSVSSEE48 pKa = 3.76 ARR50 pKa = 11.84 HH51 pKa = 5.78 KK52 pKa = 10.72 KK53 pKa = 9.83
Molecular weight: 6.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3310
0
3310
1129353
38
4718
341.2
38.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.538 ± 0.045
0.804 ± 0.015
5.682 ± 0.035
6.45 ± 0.04
5.092 ± 0.031
6.323 ± 0.044
1.967 ± 0.02
7.74 ± 0.037
7.919 ± 0.053
9.175 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.133 ± 0.018
6.194 ± 0.04
3.361 ± 0.025
3.419 ± 0.023
3.45 ± 0.026
6.458 ± 0.036
5.809 ± 0.048
6.294 ± 0.031
1.087 ± 0.015
4.105 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here