Streptococcus satellite phage Javan586

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZW72|A0A4D5ZW72_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan586 OX=2558759 GN=JavanS586_0008 PE=4 SV=1
MM1 pKa = 7.43SLKK4 pKa = 10.28QIKK7 pKa = 9.7KK8 pKa = 9.58HH9 pKa = 5.14IARR12 pKa = 11.84LDD14 pKa = 3.37EE15 pKa = 4.15LTRR18 pKa = 11.84QGGGMLLLTINPEE31 pKa = 3.56KK32 pKa = 10.57DD33 pKa = 3.5GYY35 pKa = 9.97SISYY39 pKa = 9.69SVEE42 pKa = 3.71GNSILLHH49 pKa = 5.81GFPSRR54 pKa = 11.84VEE56 pKa = 3.62AWEE59 pKa = 4.12KK60 pKa = 9.94CDD62 pKa = 4.79EE63 pKa = 4.76IIEE66 pKa = 4.18EE67 pKa = 4.51HH68 pKa = 6.6RR69 pKa = 11.84PDD71 pKa = 3.08WSMVFLDD78 pKa = 3.24EE79 pKa = 4.5WEE81 pKa = 4.42RR82 pKa = 11.84EE83 pKa = 3.91EE84 pKa = 5.92DD85 pKa = 3.94PEE87 pKa = 4.89LDD89 pKa = 3.3RR90 pKa = 11.84YY91 pKa = 10.18LEE93 pKa = 3.9KK94 pKa = 10.78RR95 pKa = 11.84KK96 pKa = 10.42LKK98 pKa = 10.12DD99 pKa = 2.99VRR101 pKa = 11.84RR102 pKa = 11.84ITFIDD107 pKa = 4.9DD108 pKa = 3.36IPLEE112 pKa = 4.36DD113 pKa = 3.51

Molecular weight: 13.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZW72|A0A4D5ZW72_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan586 OX=2558759 GN=JavanS586_0008 PE=4 SV=1
MM1 pKa = 7.61TNFQTYY7 pKa = 10.09LGYY10 pKa = 10.01GQPYY14 pKa = 10.03RR15 pKa = 11.84MADD18 pKa = 3.07EE19 pKa = 5.76HH20 pKa = 5.9IWKK23 pKa = 9.84DD24 pKa = 3.46RR25 pKa = 11.84RR26 pKa = 11.84PTLEE30 pKa = 4.79HH31 pKa = 6.51IKK33 pKa = 9.99EE34 pKa = 3.96QQRR37 pKa = 11.84LKK39 pKa = 10.78KK40 pKa = 10.24LKK42 pKa = 10.12KK43 pKa = 8.69KK44 pKa = 10.1RR45 pKa = 11.84KK46 pKa = 8.75RR47 pKa = 11.84GKK49 pKa = 9.88

Molecular weight: 6.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2140

49

532

142.7

16.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.701 ± 0.549

0.28 ± 0.115

4.673 ± 0.511

9.393 ± 0.641

3.645 ± 0.361

4.533 ± 0.498

1.776 ± 0.39

7.009 ± 0.583

9.673 ± 0.49

10.421 ± 0.633

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.15 ± 0.232

4.533 ± 0.522

3.505 ± 0.447

4.72 ± 0.648

5.701 ± 0.569

4.813 ± 0.45

6.916 ± 0.544

5.374 ± 0.574

0.794 ± 0.186

4.393 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski