Tortoise microvirus 5
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5M6|A0A4P8W5M6_9VIRU Uncharacterized protein OS=Tortoise microvirus 5 OX=2583154 PE=4 SV=1
MM1 pKa = 6.9 KK2 pKa = 9.93 TNVYY6 pKa = 10.43 SIIDD10 pKa = 3.53 SAYY13 pKa = 9.38 GRR15 pKa = 11.84 IQGVVVAPNSSLAIRR30 pKa = 11.84 DD31 pKa = 3.63 VAPMLKK37 pKa = 9.63 RR38 pKa = 11.84 VAPHH42 pKa = 6.49 FEE44 pKa = 3.4 EE45 pKa = 5.9 DD46 pKa = 3.83 MYY48 pKa = 11.12 IEE50 pKa = 4.83 CIGYY54 pKa = 10.24 YY55 pKa = 10.42 DD56 pKa = 5.74 DD57 pKa = 5.96 DD58 pKa = 5.67 LNFHH62 pKa = 6.11 ATSPEE67 pKa = 4.18 VYY69 pKa = 9.28 PWLADD74 pKa = 3.65 NPEE77 pKa = 4.25 MPMKK81 pKa = 10.17 TLTGEE86 pKa = 3.92 EE87 pKa = 4.01 AVII90 pKa = 4.28
Molecular weight: 10.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.07
IPC2_protein 4.406
IPC_protein 4.279
Toseland 4.101
ProMoST 4.406
Dawson 4.24
Bjellqvist 4.393
Wikipedia 4.139
Rodwell 4.113
Grimsley 4.024
Solomon 4.228
Lehninger 4.19
Nozaki 4.355
DTASelect 4.533
Thurlkill 4.139
EMBOSS 4.151
Sillero 4.393
Patrickios 1.99
IPC_peptide 4.228
IPC2_peptide 4.38
IPC2.peptide.svr19 4.292
Protein with the highest isoelectric point:
>tr|A0A4P8W5R7|A0A4P8W5R7_9VIRU Uncharacterized protein OS=Tortoise microvirus 5 OX=2583154 PE=4 SV=1
MM1 pKa = 7.15 LQHH4 pKa = 6.8 HH5 pKa = 6.04 SHH7 pKa = 7.28 LIKK10 pKa = 10.69 GVFSMRR16 pKa = 11.84 KK17 pKa = 7.7 TITTSISIPKK27 pKa = 9.35 DD28 pKa = 3.65 LKK30 pKa = 10.94 EE31 pKa = 3.75 KK32 pKa = 10.01 WIDD35 pKa = 3.35 KK36 pKa = 9.65 MSISISYY43 pKa = 7.55 FTSCAMKK50 pKa = 10.44 SVLRR54 pKa = 11.84 NPALLEE60 pKa = 4.24 TILFQNSLKK69 pKa = 10.68 EE70 pKa = 3.95 FF71 pKa = 4.03
Molecular weight: 8.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.903
IPC2_protein 9.019
IPC_protein 8.96
Toseland 10.058
ProMoST 9.516
Dawson 10.145
Bjellqvist 9.736
Wikipedia 10.233
Rodwell 10.935
Grimsley 10.175
Solomon 10.189
Lehninger 10.189
Nozaki 10.043
DTASelect 9.706
Thurlkill 10.028
EMBOSS 10.409
Sillero 10.072
Patrickios 10.774
IPC_peptide 10.204
IPC2_peptide 8.214
IPC2.peptide.svr19 8.005
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1659
71
627
237.0
26.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.691 ± 0.705
1.206 ± 0.469
6.57 ± 1.132
4.159 ± 0.505
4.882 ± 0.612
6.45 ± 0.809
1.567 ± 0.445
5.726 ± 0.48
5.184 ± 0.903
8.74 ± 0.873
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.592 ± 0.389
5.063 ± 0.404
4.28 ± 0.82
3.918 ± 1.086
5.726 ± 0.877
10.488 ± 0.838
5.546 ± 0.674
5.003 ± 0.507
1.206 ± 0.287
5.003 ± 0.739
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here