Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Desulfurococcales; Pyrodictiaceae; Hyperthermus; Hyperthermus butylicus

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1602 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A2BMJ1|A2BMJ1_HYPBU 4Fe-4S ferredoxin-type domain-containing protein OS=Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5) OX=415426 GN=Hbut_1377 PE=4 SV=1
MM1 pKa = 7.47APEE4 pKa = 3.71EE5 pKa = 4.93AAWCWMWARR14 pKa = 11.84PFYY17 pKa = 10.86VVYY20 pKa = 10.74NVTYY24 pKa = 10.55CFMAACEE31 pKa = 4.24DD32 pKa = 3.61AGEE35 pKa = 4.32EE36 pKa = 4.13YY37 pKa = 10.17MAVITNVKK45 pKa = 10.27SDD47 pKa = 3.25GLYY50 pKa = 9.8ISGSVGFGLPPEE62 pKa = 4.66TIMNALLSGTEE73 pKa = 4.0SNIVFISNTPLADD86 pKa = 4.03GDD88 pKa = 4.32LDD90 pKa = 3.96PGEE93 pKa = 4.63FVDD96 pKa = 5.53LRR98 pKa = 11.84DD99 pKa = 3.27IFRR102 pKa = 11.84SYY104 pKa = 9.22DD105 pKa = 3.06TCGADD110 pKa = 3.63FEE112 pKa = 4.81IGIPIGAMLAVPACAALGLPTGGAACAALTAFMSSFAVTISAEE155 pKa = 4.36SPSVTVYY162 pKa = 11.38GNIGNLGDD170 pKa = 3.82TPSIEE175 pKa = 3.81NDD177 pKa = 3.33VNVFEE182 pKa = 4.19YY183 pKa = 11.11VYY185 pKa = 9.86MAEE188 pKa = 3.78SRR190 pKa = 11.84YY191 pKa = 10.14KK192 pKa = 10.28YY193 pKa = 10.79VYY195 pKa = 10.39GDD197 pKa = 3.45CEE199 pKa = 4.49YY200 pKa = 10.41NVPVGMVIEE209 pKa = 4.53FRR211 pKa = 3.93

Molecular weight: 22.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A2BN64|RL1_HYPBU 50S ribosomal protein L1 OS=Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5) OX=415426 GN=rpl1 PE=3 SV=1
MM1 pKa = 7.51AHH3 pKa = 6.89FKK5 pKa = 10.66PLGKK9 pKa = 10.13KK10 pKa = 9.79LRR12 pKa = 11.84LAKK15 pKa = 10.28ALKK18 pKa = 9.77QNRR21 pKa = 11.84SVPIWVIIKK30 pKa = 6.92TNRR33 pKa = 11.84RR34 pKa = 11.84FRR36 pKa = 11.84DD37 pKa = 3.48HH38 pKa = 7.22PKK40 pKa = 8.53RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.73WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.87LKK50 pKa = 10.48AA51 pKa = 3.35

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1602

0

1602

449830

44

1507

280.8

31.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.062 ± 0.092

0.922 ± 0.028

4.187 ± 0.039

7.68 ± 0.07

2.712 ± 0.039

7.62 ± 0.058

1.79 ± 0.025

6.171 ± 0.057

4.507 ± 0.069

11.138 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.024

2.166 ± 0.03

5.062 ± 0.038

1.999 ± 0.029

7.811 ± 0.067

5.302 ± 0.05

4.399 ± 0.045

9.215 ± 0.055

1.24 ± 0.026

3.907 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski