Roseibacterium elongatum DSM 19469
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3433 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8RU49|W8RU49_9RHOB Serine acetyltransferase OS=Roseibacterium elongatum DSM 19469 OX=1294273 GN=roselon_02429 PE=3 SV=1
MM1 pKa = 7.4 AAAPGTGGGGGGTGTGGTGGTGGGGTGDD29 pKa = 4.08 PGTGGGGTGGTGGGGTGGTGGGTGGGTTDD58 pKa = 4.59 PDD60 pKa = 3.86 PVTPYY65 pKa = 10.26 IPTSPVVGCPSTDD78 pKa = 3.64 YY79 pKa = 11.17 LGEE82 pKa = 4.64 AYY84 pKa = 7.07 TTTGEE89 pKa = 4.13 DD90 pKa = 4.13 LEE92 pKa = 5.57 DD93 pKa = 3.26 EE94 pKa = 4.64 TVRR97 pKa = 11.84 LDD99 pKa = 3.76 ASGGGQTPLDD109 pKa = 4.27 SCPGIPGTGYY119 pKa = 10.91 VYY121 pKa = 11.07 ANPTFSLYY129 pKa = 10.63 LSEE132 pKa = 4.56 MDD134 pKa = 3.47 DD135 pKa = 3.68 FEE137 pKa = 5.75 RR138 pKa = 11.84 FVVRR142 pKa = 11.84 IHH144 pKa = 6.93 SDD146 pKa = 3.21 CDD148 pKa = 3.36 TTLLLRR154 pKa = 11.84 DD155 pKa = 3.61 AQGNYY160 pKa = 10.12 HH161 pKa = 6.78 FDD163 pKa = 4.42 DD164 pKa = 5.72 DD165 pKa = 5.58 SGPDD169 pKa = 3.23 LNGRR173 pKa = 11.84 LRR175 pKa = 11.84 LRR177 pKa = 11.84 DD178 pKa = 3.78 MEE180 pKa = 4.54 PLNGRR185 pKa = 11.84 VDD187 pKa = 3.58 IYY189 pKa = 11.56 VGTFDD194 pKa = 6.03 GDD196 pKa = 3.59 TCNDD200 pKa = 3.22 VQVRR204 pKa = 11.84 LILRR208 pKa = 11.84 DD209 pKa = 3.35
Molecular weight: 20.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.579
ProMoST 3.948
Dawson 3.821
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.63
Grimsley 3.478
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.228
Thurlkill 3.643
EMBOSS 3.795
Sillero 3.935
Patrickios 1.1
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|W8S5X5|W8S5X5_9RHOB Uncharacterized protein OS=Roseibacterium elongatum DSM 19469 OX=1294273 GN=roselon_03379 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 KK14 pKa = 9.18 RR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.42 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.37 AGRR29 pKa = 11.84 KK30 pKa = 8.54 ILNARR35 pKa = 11.84 RR36 pKa = 11.84 ARR38 pKa = 11.84 GRR40 pKa = 11.84 KK41 pKa = 9.07 SLSAA45 pKa = 3.93
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3433
0
3433
1011806
37
1857
294.7
31.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.112 ± 0.064
0.896 ± 0.014
6.327 ± 0.049
5.753 ± 0.039
3.584 ± 0.03
9.064 ± 0.049
2.113 ± 0.025
4.948 ± 0.031
2.416 ± 0.039
10.07 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.79 ± 0.02
2.223 ± 0.025
5.485 ± 0.037
3.031 ± 0.022
7.291 ± 0.05
4.67 ± 0.029
5.473 ± 0.03
7.209 ± 0.036
1.433 ± 0.017
2.113 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here