Pseudonocardia sp. CNS-139

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia; unclassified Pseudonocardia

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4969 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9T9Y1|A0A1Q9T9Y1_9PSEU DUF1508 domain-containing protein OS=Pseudonocardia sp. CNS-139 OX=1904968 GN=BJF78_10100 PE=4 SV=1
MM1 pKa = 7.36IRR3 pKa = 11.84RR4 pKa = 11.84PALMVATAAAAALVLSACGGAEE26 pKa = 4.03EE27 pKa = 4.65PAAPAAPAAPAAPAGGPLDD46 pKa = 3.85LAGVCPATVVLQQDD60 pKa = 3.62WQPEE64 pKa = 4.25AEE66 pKa = 4.43HH67 pKa = 6.25GAMYY71 pKa = 11.02ALVGPDD77 pKa = 3.31YY78 pKa = 11.07TIDD81 pKa = 3.65ADD83 pKa = 3.93AKK85 pKa = 10.1SVTGSLVTQGVDD97 pKa = 2.68TGVDD101 pKa = 3.45VEE103 pKa = 4.47VRR105 pKa = 11.84PGGPNVGFQPVPALMYY121 pKa = 10.52LDD123 pKa = 5.02DD124 pKa = 6.4AITLGAVNTDD134 pKa = 3.3AAIVAAADD142 pKa = 3.56QPTVAVASQMTVSPQILMWDD162 pKa = 4.08PEE164 pKa = 4.52SHH166 pKa = 7.11PGMTTIRR173 pKa = 11.84QIAATGAPVVTSGDD187 pKa = 3.8IIPALLEE194 pKa = 4.01SQGIIQASQSDD205 pKa = 3.83TSYY208 pKa = 10.57EE209 pKa = 3.93GTPARR214 pKa = 11.84FVSDD218 pKa = 3.74PVILQQGFATAEE230 pKa = 3.88PYY232 pKa = 10.0IYY234 pKa = 10.35QNEE237 pKa = 3.99IGEE240 pKa = 4.19WSRR243 pKa = 11.84PVGFEE248 pKa = 3.35PLANYY253 pKa = 9.58GYY255 pKa = 10.47SIYY258 pKa = 10.06PEE260 pKa = 4.24PLAVRR265 pKa = 11.84ADD267 pKa = 3.82RR268 pKa = 11.84LEE270 pKa = 3.61QLRR273 pKa = 11.84PCLEE277 pKa = 3.84KK278 pKa = 10.63LVPIMQQSQIDD289 pKa = 4.04YY290 pKa = 10.17LADD293 pKa = 4.32PGPTNRR299 pKa = 11.84LIVEE303 pKa = 4.36LVEE306 pKa = 5.01AYY308 pKa = 7.53QTGWTYY314 pKa = 11.47SEE316 pKa = 4.52GVADD320 pKa = 6.41FSAQAQVEE328 pKa = 4.42QGFVTNDD335 pKa = 3.23PVSGVFGQFDD345 pKa = 4.18PARR348 pKa = 11.84MAEE351 pKa = 4.12IVSTFGPILQSQGTISSVPDD371 pKa = 3.61ASALYY376 pKa = 10.0TNDD379 pKa = 5.43FIDD382 pKa = 3.64TTIKK386 pKa = 10.07MGG388 pKa = 3.51

Molecular weight: 40.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9SNR2|A0A1Q9SNR2_9PSEU Uncharacterized protein OS=Pseudonocardia sp. CNS-139 OX=1904968 GN=BJF78_24850 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84THH19 pKa = 5.69GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84AKK40 pKa = 10.14GRR42 pKa = 11.84QRR44 pKa = 11.84LSAA47 pKa = 4.04

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4969

0

4969

1162861

30

1898

234.0

24.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.819 ± 0.059

0.911 ± 0.014

5.857 ± 0.036

5.031 ± 0.037

2.558 ± 0.021

9.593 ± 0.041

2.126 ± 0.019

2.75 ± 0.025

1.094 ± 0.017

10.155 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.718 ± 0.015

1.458 ± 0.018

6.77 ± 0.036

2.476 ± 0.022

9.126 ± 0.05

4.924 ± 0.033

5.884 ± 0.028

9.52 ± 0.037

1.53 ± 0.016

1.7 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski