Massilia psychrophila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4079 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G8T273|A0A2G8T273_9BURK Uncharacterized protein OS=Massilia psychrophila OX=1603353 GN=CR103_09075 PE=4 SV=1
MM1 pKa = 7.1NAVAEE6 pKa = 4.24MQEE9 pKa = 4.64VIPSPIIFTDD19 pKa = 3.54SAAQKK24 pKa = 9.24VAQLIEE30 pKa = 4.37EE31 pKa = 4.51EE32 pKa = 4.63GNPDD36 pKa = 2.85LKK38 pKa = 11.13LRR40 pKa = 11.84VFVQGGGCSGFQYY53 pKa = 10.94GFTFDD58 pKa = 4.69EE59 pKa = 4.93IVNEE63 pKa = 4.83DD64 pKa = 3.85DD65 pKa = 3.11TTMVKK70 pKa = 10.58NGVQLLIDD78 pKa = 3.77SMSYY82 pKa = 10.23QYY84 pKa = 11.55LVGAEE89 pKa = 4.01IDD91 pKa = 3.89YY92 pKa = 11.28KK93 pKa = 11.39DD94 pKa = 4.47DD95 pKa = 4.08LEE97 pKa = 4.56GAQFVIKK104 pKa = 10.51NPSATSTCGCGSSFSAA120 pKa = 4.79

Molecular weight: 12.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G8T4K2|A0A2G8T4K2_9BURK Uncharacterized protein OS=Massilia psychrophila OX=1603353 GN=CR103_04410 PE=4 SV=1
MM1 pKa = 7.61LLHH4 pKa = 6.94LSSAHH9 pKa = 5.71SAVDD13 pKa = 3.4RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84CEE18 pKa = 3.6VAIRR22 pKa = 11.84AWAARR27 pKa = 11.84DD28 pKa = 3.38KK29 pKa = 10.94HH30 pKa = 5.78VQALLHH36 pKa = 6.37RR37 pKa = 11.84VDD39 pKa = 3.91ALRR42 pKa = 11.84MDD44 pKa = 4.97FARR47 pKa = 11.84EE48 pKa = 3.79SARR51 pKa = 11.84AVPPCPVRR59 pKa = 11.84GADD62 pKa = 3.16IEE64 pKa = 5.09AFAQLAYY71 pKa = 9.68LTVLYY76 pKa = 10.39VWIRR80 pKa = 11.84GGLCGRR86 pKa = 11.84NQKK89 pKa = 10.18SSSWVKK95 pKa = 9.8FLYY98 pKa = 10.76APVPGRR104 pKa = 11.84LFSKK108 pKa = 10.82

Molecular weight: 12.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4079

0

4079

1339801

26

2860

328.5

35.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.851 ± 0.049

0.937 ± 0.013

5.387 ± 0.029

4.943 ± 0.033

3.699 ± 0.025

8.195 ± 0.037

2.187 ± 0.019

4.888 ± 0.025

3.715 ± 0.033

10.392 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.019

3.106 ± 0.025

4.893 ± 0.026

3.809 ± 0.023

6.45 ± 0.034

5.588 ± 0.031

5.178 ± 0.029

7.438 ± 0.029

1.281 ± 0.016

2.422 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski