Arthrobacter phage Yang
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G2KJF8|A0A3G2KJF8_9CAUD DNA primase/helicase OS=Arthrobacter phage Yang OX=2419970 GN=38 PE=4 SV=1
MM1 pKa = 7.85 DD2 pKa = 6.29 LDD4 pKa = 5.22 DD5 pKa = 7.18 DD6 pKa = 4.93 IDD8 pKa = 5.43 LEE10 pKa = 4.54 LDD12 pKa = 3.06 PRR14 pKa = 11.84 EE15 pKa = 4.31 YY16 pKa = 11.09 VCAACRR22 pKa = 11.84 LTHH25 pKa = 6.13 WRR27 pKa = 11.84 GAPDD31 pKa = 3.72 PCDD34 pKa = 3.11 RR35 pKa = 11.84 AA36 pKa = 3.65
Molecular weight: 4.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.085
IPC2_protein 4.075
IPC_protein 3.986
Toseland 3.77
ProMoST 4.19
Dawson 3.999
Bjellqvist 4.164
Wikipedia 3.986
Rodwell 3.821
Grimsley 3.681
Solomon 3.986
Lehninger 3.935
Nozaki 4.139
DTASelect 4.406
Thurlkill 3.846
EMBOSS 3.986
Sillero 4.113
Patrickios 3.198
IPC_peptide 3.973
IPC2_peptide 4.088
IPC2.peptide.svr19 4.033
Protein with the highest isoelectric point:
>tr|A0A3G2KJH4|A0A3G2KJH4_9CAUD Endolysin OS=Arthrobacter phage Yang OX=2419970 GN=59 PE=4 SV=1
MM1 pKa = 8.24 VEE3 pKa = 4.26 EE4 pKa = 4.22 VTPAARR10 pKa = 11.84 IAQAAQAFDD19 pKa = 3.78 SGNGEE24 pKa = 4.05 AQALTAQALARR35 pKa = 11.84 LAYY38 pKa = 9.81 RR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 HH42 pKa = 5.55 SGKK45 pKa = 7.69 TCQTCRR51 pKa = 11.84 EE52 pKa = 4.33 TKK54 pKa = 9.67 PLSAFGFDD62 pKa = 3.64 ALRR65 pKa = 11.84 PDD67 pKa = 4.04 GLSVRR72 pKa = 11.84 CRR74 pKa = 11.84 ACNSARR80 pKa = 11.84 MRR82 pKa = 11.84 ASRR85 pKa = 11.84 AARR88 pKa = 11.84 VNVPQPGQPSLMM100 pKa = 4.21
Molecular weight: 10.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.443
IPC_protein 10.613
Toseland 10.774
ProMoST 10.833
Dawson 10.818
Bjellqvist 10.687
Wikipedia 11.14
Rodwell 10.73
Grimsley 10.862
Solomon 11.111
Lehninger 11.052
Nozaki 10.818
DTASelect 10.657
Thurlkill 10.774
EMBOSS 11.213
Sillero 10.804
Patrickios 10.57
IPC_peptide 11.125
IPC2_peptide 10.233
IPC2.peptide.svr19 8.905
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
0
68
13449
36
967
197.8
21.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.399 ± 0.468
0.595 ± 0.164
6.588 ± 0.391
5.926 ± 0.398
3.041 ± 0.182
8.529 ± 0.462
1.829 ± 0.191
4.164 ± 0.286
3.985 ± 0.294
8.61 ± 0.293
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.74 ± 0.113
2.253 ± 0.178
5.071 ± 0.281
2.997 ± 0.205
7.517 ± 0.559
5.614 ± 0.317
6.566 ± 0.419
7.279 ± 0.379
1.785 ± 0.122
2.513 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here