Aquabacterium commune
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3649 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R6RPX5|A0A4R6RPX5_9BURK Diguanylate cyclase (GGDEF)-like protein OS=Aquabacterium commune OX=70586 GN=EV672_101332 PE=4 SV=1
MM1 pKa = 7.28 NPSDD5 pKa = 4.58 VSDD8 pKa = 4.11 LSTWMCLICGWIYY21 pKa = 11.22 DD22 pKa = 4.06 EE23 pKa = 5.24 AAGLPEE29 pKa = 4.46 EE30 pKa = 5.49 GIAPGTRR37 pKa = 11.84 WADD40 pKa = 3.22 VPMNWVCPEE49 pKa = 3.74 CGARR53 pKa = 11.84 KK54 pKa = 9.81 DD55 pKa = 3.83 DD56 pKa = 4.18 FEE58 pKa = 4.39 MVPVV62 pKa = 3.92
Molecular weight: 6.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.808
IPC_protein 3.681
Toseland 3.49
ProMoST 3.872
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.414
Solomon 3.643
Lehninger 3.605
Nozaki 3.834
DTASelect 3.999
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.808
Patrickios 0.604
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|A0A4R6RAD6|A0A4R6RAD6_9BURK Putative secreted hydrolase OS=Aquabacterium commune OX=70586 GN=EV672_105166 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.25 QPSKK9 pKa = 7.79 TRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.79 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.67 TKK25 pKa = 10.38 GGRR28 pKa = 11.84 KK29 pKa = 9.05 VISARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.85 GRR39 pKa = 11.84 KK40 pKa = 9.07 RR41 pKa = 11.84 LGLL44 pKa = 3.7
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3649
0
3649
1271893
25
4944
348.6
37.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.802 ± 0.059
0.914 ± 0.014
5.404 ± 0.029
5.082 ± 0.04
3.274 ± 0.026
8.122 ± 0.043
2.524 ± 0.027
3.963 ± 0.029
3.242 ± 0.036
10.807 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.43 ± 0.022
2.532 ± 0.027
5.323 ± 0.035
4.551 ± 0.03
6.89 ± 0.041
5.577 ± 0.031
5.377 ± 0.034
7.698 ± 0.032
1.549 ± 0.02
1.938 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here