Streptococcus phage Javan68
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6BD53|A0A4D6BD53_9CAUD Tail assembly protein OS=Streptococcus phage Javan68 OX=2548301 GN=Javan68_0005 PE=4 SV=1
MM1 pKa = 7.47 HH2 pKa = 7.02 GKK4 pKa = 9.97 KK5 pKa = 10.18 YY6 pKa = 11.22 YY7 pKa = 10.87 EE8 pKa = 4.36 MDD10 pKa = 4.06 DD11 pKa = 4.32 EE12 pKa = 5.73 INDD15 pKa = 3.6 VPHH18 pKa = 7.01 FGLTYY23 pKa = 10.58 SEE25 pKa = 5.04 FEE27 pKa = 4.04 GDD29 pKa = 3.09 DD30 pKa = 3.28 WVIGFDD36 pKa = 4.01 CNHH39 pKa = 6.74 AFDD42 pKa = 4.46 TPATNTVEE50 pKa = 4.26 FVEE53 pKa = 4.92 GNIKK57 pKa = 10.43 EE58 pKa = 4.22 IVDD61 pKa = 4.77 TILEE65 pKa = 4.3 IYY67 pKa = 10.22 PEE69 pKa = 4.71 GEE71 pKa = 3.79
Molecular weight: 8.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.935
IPC_protein 3.846
Toseland 3.668
ProMoST 3.961
Dawson 3.821
Bjellqvist 4.05
Wikipedia 3.732
Rodwell 3.694
Grimsley 3.579
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.719
EMBOSS 3.745
Sillero 3.973
Patrickios 0.846
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.891
Protein with the highest isoelectric point:
>tr|A0A4D6BHF2|A0A4D6BHF2_9CAUD Tail length tape-measure protein OS=Streptococcus phage Javan68 OX=2548301 GN=Javan68_0007 PE=4 SV=1
MM1 pKa = 7.92 PISFLKK7 pKa = 10.46 RR8 pKa = 11.84 SCPEE12 pKa = 3.38 VAFIEE17 pKa = 4.26 RR18 pKa = 11.84 KK19 pKa = 8.7 KK20 pKa = 10.96 IKK22 pKa = 9.46 MKK24 pKa = 8.97 YY25 pKa = 8.59 TKK27 pKa = 9.33 TKK29 pKa = 9.21 YY30 pKa = 9.68 PNIYY34 pKa = 9.56 TYY36 pKa = 8.37 EE37 pKa = 4.05 TTKK40 pKa = 10.58 GKK42 pKa = 10.05 RR43 pKa = 11.84 YY44 pKa = 7.98 YY45 pKa = 10.47 VRR47 pKa = 11.84 RR48 pKa = 11.84 SFMFQGKK55 pKa = 8.49 KK56 pKa = 9.49 QEE58 pKa = 4.29 ANKK61 pKa = 10.58 SSLKK65 pKa = 9.91 TISEE69 pKa = 4.01 ARR71 pKa = 11.84 AEE73 pKa = 3.85 LAKK76 pKa = 10.16 IEE78 pKa = 4.76 KK79 pKa = 10.07 RR80 pKa = 11.84 IADD83 pKa = 3.43 QTIAINPNVTVEE95 pKa = 4.29 EE96 pKa = 4.36 YY97 pKa = 9.87 WEE99 pKa = 3.94 KK100 pKa = 10.55 FYY102 pKa = 11.24 EE103 pKa = 4.17 KK104 pKa = 10.03 RR105 pKa = 11.84 VEE107 pKa = 4.14 TGRR110 pKa = 11.84 WTPNTEE116 pKa = 3.72 VYY118 pKa = 10.56 YY119 pKa = 10.94 KK120 pKa = 9.96 STFKK124 pKa = 11.07 LHH126 pKa = 7.08 ILPFWGKK133 pKa = 8.05 TKK135 pKa = 10.8 LKK137 pKa = 9.7 NLSRR141 pKa = 11.84 NEE143 pKa = 4.09 YY144 pKa = 8.91 EE145 pKa = 3.86 KK146 pKa = 10.96 HH147 pKa = 5.37 IANLLKK153 pKa = 10.4 VRR155 pKa = 11.84 PKK157 pKa = 9.53 ASVRR161 pKa = 11.84 IIHH164 pKa = 5.87 SCFMTMLNDD173 pKa = 4.9 AIMNGNISANRR184 pKa = 11.84 LNGIYY189 pKa = 10.61 VGDD192 pKa = 3.78 SLIAKK197 pKa = 8.69 KK198 pKa = 10.43 NKK200 pKa = 10.08 RR201 pKa = 11.84 ITLDD205 pKa = 3.15 QFQIWMQEE213 pKa = 3.72 AEE215 pKa = 4.24 KK216 pKa = 11.34 VMDD219 pKa = 3.38 KK220 pKa = 10.8 TFFGLTYY227 pKa = 10.56 LAIFGLRR234 pKa = 11.84 RR235 pKa = 11.84 GEE237 pKa = 3.9 ILGLRR242 pKa = 11.84 HH243 pKa = 6.65 CDD245 pKa = 3.22 VNINVNGRR253 pKa = 11.84 AVLHH257 pKa = 6.04 LQDD260 pKa = 4.48 SRR262 pKa = 11.84 GNHH265 pKa = 5.01 TKK267 pKa = 10.55 NGRR270 pKa = 11.84 HH271 pKa = 4.77 GLKK274 pKa = 9.47 TKK276 pKa = 10.63 SSEE279 pKa = 3.84 RR280 pKa = 11.84 WVTLDD285 pKa = 3.23 NTGTEE290 pKa = 4.28 LLLSLMSNAEE300 pKa = 4.01 AVKK303 pKa = 10.59 KK304 pKa = 9.99 KK305 pKa = 10.82 LNIIKK310 pKa = 9.89 EE311 pKa = 4.24 VEE313 pKa = 3.46 WDD315 pKa = 4.07 YY316 pKa = 11.84 ISIDD320 pKa = 3.38 QKK322 pKa = 11.51 GRR324 pKa = 11.84 LINPNKK330 pKa = 10.15 LNRR333 pKa = 11.84 EE334 pKa = 4.08 FEE336 pKa = 4.24 RR337 pKa = 11.84 VNDD340 pKa = 3.58 VVGFRR345 pKa = 11.84 VTPHH349 pKa = 5.79 MLRR352 pKa = 11.84 HH353 pKa = 6.02 FFTTQSIIAGVPMEE367 pKa = 5.08 ALSQALGHH375 pKa = 6.09 TKK377 pKa = 9.66 MYY379 pKa = 7.84 MTDD382 pKa = 3.49 KK383 pKa = 10.69 YY384 pKa = 11.15 NQVHH388 pKa = 7.18 DD389 pKa = 4.28 EE390 pKa = 4.27 LSAQVSDD397 pKa = 4.41 AFIAQISPRR406 pKa = 11.84 NSPTNLHH413 pKa = 6.35 ILL415 pKa = 3.73
Molecular weight: 48.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.059
IPC2_protein 9.238
IPC_protein 9.165
Toseland 10.028
ProMoST 9.648
Dawson 10.204
Bjellqvist 9.838
Wikipedia 10.335
Rodwell 10.73
Grimsley 10.262
Solomon 10.218
Lehninger 10.189
Nozaki 10.014
DTASelect 9.823
Thurlkill 10.058
EMBOSS 10.423
Sillero 10.116
Patrickios 10.321
IPC_peptide 10.218
IPC2_peptide 8.317
IPC2.peptide.svr19 8.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
11191
38
1429
243.3
27.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.087 ± 0.639
0.608 ± 0.116
5.987 ± 0.371
7.819 ± 0.328
3.628 ± 0.313
6.738 ± 0.329
1.287 ± 0.095
5.987 ± 0.257
8.435 ± 0.277
8.283 ± 0.343
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.359 ± 0.162
5.719 ± 0.281
3.101 ± 0.218
4.218 ± 0.251
4.298 ± 0.282
6.05 ± 0.317
5.987 ± 0.308
6.148 ± 0.258
1.394 ± 0.169
3.869 ± 0.336
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here