Amino acid dipepetide frequency for Microbacterium sp. dk485

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.927AlaAla: 22.927 ± 0.236	0.705AlaCys: 0.705 ± 0.026	8.826AlaAsp: 8.826 ± 0.083	8.562AlaGlu: 8.562 ± 0.095	4.049AlaPhe: 4.049 ± 0.066	13.311AlaGly: 13.311 ± 0.12	2.681AlaHis: 2.681 ± 0.053	5.637AlaIle: 5.637 ± 0.078	2.063AlaLys: 2.063 ± 0.056	14.93AlaLeu: 14.93 ± 0.141	2.759AlaMet: 2.759 ± 0.052	2.009AlaAsn: 2.009 ± 0.043	7.153AlaPro: 7.153 ± 0.099	3.903AlaGln: 3.903 ± 0.053	10.503AlaArg: 10.503 ± 0.128	7.101AlaSer: 7.101 ± 0.073	7.471AlaThr: 7.471 ± 0.078	12.86AlaVal: 12.86 ± 0.133	2.157AlaTrp: 2.157 ± 0.049	2.582AlaTyr: 2.582 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.728CysAla: 0.728 ± 0.028	0.041CysCys: 0.041 ± 0.006	0.334CysAsp: 0.334 ± 0.018	0.249CysGlu: 0.249 ± 0.016	0.161CysPhe: 0.161 ± 0.013	0.522CysGly: 0.522 ± 0.023	0.104CysHis: 0.104 ± 0.01	0.18CysIle: 0.18 ± 0.015	0.051CysLys: 0.051 ± 0.007	0.458CysLeu: 0.458 ± 0.022	0.072CysMet: 0.072 ± 0.007	0.087CysAsn: 0.087 ± 0.009	0.275CysPro: 0.275 ± 0.016	0.116CysGln: 0.116 ± 0.011	0.321CysArg: 0.321 ± 0.018	0.291CysSer: 0.291 ± 0.016	0.312CysThr: 0.312 ± 0.018	0.427CysVal: 0.427 ± 0.019	0.075CysTrp: 0.075 ± 0.009	0.098CysTyr: 0.098 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	9.94AspAla: 9.94 ± 0.105	0.221AspCys: 0.221 ± 0.014	4.327AspAsp: 4.327 ± 0.078	4.114AspGlu: 4.114 ± 0.062	1.628AspPhe: 1.628 ± 0.039	6.015AspGly: 6.015 ± 0.084	1.091AspHis: 1.091 ± 0.032	2.4AspIle: 2.4 ± 0.045	0.797AspLys: 0.797 ± 0.03	6.295AspLeu: 6.295 ± 0.066	0.858AspMet: 0.858 ± 0.029	0.822AspAsn: 0.822 ± 0.03	4.56AspPro: 4.56 ± 0.065	1.342AspGln: 1.342 ± 0.032	4.606AspArg: 4.606 ± 0.072	2.462AspSer: 2.462 ± 0.044	2.911AspThr: 2.911 ± 0.049	5.785AspVal: 5.785 ± 0.069	0.833AspTrp: 0.833 ± 0.03	1.229AspTyr: 1.229 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	7.138GluAla: 7.138 ± 0.109	0.246GluCys: 0.246 ± 0.017	2.89GluAsp: 2.89 ± 0.051	3.251GluGlu: 3.251 ± 0.055	1.703GluPhe: 1.703 ± 0.039	4.349GluGly: 4.349 ± 0.075	1.358GluHis: 1.358 ± 0.037	2.585GluIle: 2.585 ± 0.057	1.158GluLys: 1.158 ± 0.038	6.227GluLeu: 6.227 ± 0.074	0.886GluMet: 0.886 ± 0.033	1.124GluAsn: 1.124 ± 0.032	3.1GluPro: 3.1 ± 0.056	1.978GluGln: 1.978 ± 0.047	5.562GluArg: 5.562 ± 0.086	2.86GluSer: 2.86 ± 0.054	2.819GluThr: 2.819 ± 0.053	4.754GluVal: 4.754 ± 0.07	0.95GluTrp: 0.95 ± 0.029	1.215GluTyr: 1.215 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.522PheAla: 4.522 ± 0.067	0.138PheCys: 0.138 ± 0.011	2.342PheAsp: 2.342 ± 0.046	1.568PheGlu: 1.568 ± 0.036	1.072PhePhe: 1.072 ± 0.039	3.371PheGly: 3.371 ± 0.061	0.516PheHis: 0.516 ± 0.022	1.032PheIle: 1.032 ± 0.034	0.323PheLys: 0.323 ± 0.019	2.971PheLeu: 2.971 ± 0.056	0.378PheMet: 0.378 ± 0.018	0.586PheAsn: 0.586 ± 0.025	1.514PhePro: 1.514 ± 0.039	0.765PheGln: 0.765 ± 0.023	1.908PheArg: 1.908 ± 0.051	1.629PheSer: 1.629 ± 0.04	2.079PheThr: 2.079 ± 0.045	2.865PheVal: 2.865 ± 0.057	0.497PheTrp: 0.497 ± 0.023	0.644PheTyr: 0.644 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	11.549GlyAla: 11.549 ± 0.108	0.554GlyCys: 0.554 ± 0.021	5.289GlyAsp: 5.289 ± 0.078	5.176GlyGlu: 5.176 ± 0.07	3.229GlyPhe: 3.229 ± 0.055	8.017GlyGly: 8.017 ± 0.109	1.747GlyHis: 1.747 ± 0.039	4.668GlyIle: 4.668 ± 0.071	1.773GlyLys: 1.773 ± 0.042	8.71GlyLeu: 8.71 ± 0.089	2.123GlyMet: 2.123 ± 0.046	1.555GlyAsn: 1.555 ± 0.043	4.104GlyPro: 4.104 ± 0.059	2.356GlyGln: 2.356 ± 0.047	6.807GlyArg: 6.807 ± 0.085	4.978GlySer: 4.978 ± 0.06	5.492GlyThr: 5.492 ± 0.083	8.277GlyVal: 8.277 ± 0.094	1.758GlyTrp: 1.758 ± 0.047	2.181GlyTyr: 2.181 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.577HisAla: 2.577 ± 0.051	0.12HisCys: 0.12 ± 0.011	1.35HisAsp: 1.35 ± 0.032	1.07HisGlu: 1.07 ± 0.03	0.532HisPhe: 0.532 ± 0.023	1.94HisGly: 1.94 ± 0.042	0.522HisHis: 0.522 ± 0.022	0.673HisIle: 0.673 ± 0.023	0.229HisLys: 0.229 ± 0.015	2.027HisLeu: 2.027 ± 0.042	0.324HisMet: 0.324 ± 0.015	0.321HisAsn: 0.321 ± 0.018	1.589HisPro: 1.589 ± 0.041	0.434HisGln: 0.434 ± 0.017	1.58HisArg: 1.58 ± 0.038	0.912HisSer: 0.912 ± 0.031	1.029HisThr: 1.029 ± 0.031	1.862HisVal: 1.862 ± 0.044	0.282HisTrp: 0.282 ± 0.016	0.387HisTyr: 0.387 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	6.806IleAla: 6.806 ± 0.08	0.218IleCys: 0.218 ± 0.013	3.203IleAsp: 3.203 ± 0.055	2.537IleGlu: 2.537 ± 0.043	1.082IlePhe: 1.082 ± 0.035	4.3IleGly: 4.3 ± 0.065	0.7IleHis: 0.7 ± 0.023	1.511IleIle: 1.511 ± 0.043	0.572IleLys: 0.572 ± 0.026	3.859IleLeu: 3.859 ± 0.055	0.541IleMet: 0.541 ± 0.022	0.783IleAsn: 0.783 ± 0.03	2.505IlePro: 2.505 ± 0.05	0.965IleGln: 0.965 ± 0.032	2.75IleArg: 2.75 ± 0.05	2.099IleSer: 2.099 ± 0.042	2.417IleThr: 2.417 ± 0.053	4.373IleVal: 4.373 ± 0.06	0.472IleTrp: 0.472 ± 0.021	0.672IleTyr: 0.672 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	1.933LysAla: 1.933 ± 0.051	0.053LysCys: 0.053 ± 0.007	0.862LysAsp: 0.862 ± 0.031	0.799LysGlu: 0.799 ± 0.027	0.389LysPhe: 0.389 ± 0.02	1.257LysGly: 1.257 ± 0.039	0.32LysHis: 0.32 ± 0.018	0.711LysIle: 0.711 ± 0.028	0.51LysLys: 0.51 ± 0.028	1.427LysLeu: 1.427 ± 0.045	0.294LysMet: 0.294 ± 0.019	0.385LysAsn: 0.385 ± 0.02	0.939LysPro: 0.939 ± 0.031	0.497LysGln: 0.497 ± 0.019	1.21LysArg: 1.21 ± 0.034	0.857LysSer: 0.857 ± 0.033	1.062LysThr: 1.062 ± 0.034	1.334LysVal: 1.334 ± 0.04	0.207LysTrp: 0.207 ± 0.015	0.362LysTyr: 0.362 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	15.341LeuAla: 15.341 ± 0.152	0.489LeuCys: 0.489 ± 0.02	6.446LeuAsp: 6.446 ± 0.081	5.278LeuGlu: 5.278 ± 0.07	2.873LeuPhe: 2.873 ± 0.061	9.099LeuGly: 9.099 ± 0.102	1.917LeuHis: 1.917 ± 0.041	4.182LeuIle: 4.182 ± 0.069	1.379LeuLys: 1.379 ± 0.038	10.4LeuLeu: 10.4 ± 0.119	1.554LeuMet: 1.554 ± 0.04	1.668LeuAsn: 1.668 ± 0.038	5.777LeuPro: 5.777 ± 0.077	2.627LeuGln: 2.627 ± 0.044	7.989LeuArg: 7.989 ± 0.104	5.856LeuSer: 5.856 ± 0.065	6.338LeuThr: 6.338 ± 0.069	9.441LeuVal: 9.441 ± 0.1	1.338LeuTrp: 1.338 ± 0.033	1.66LeuTyr: 1.66 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	2.164MetAla: 2.164 ± 0.045	0.092MetCys: 0.092 ± 0.009	0.852MetAsp: 0.852 ± 0.031	0.594MetGlu: 0.594 ± 0.026	0.516MetPhe: 0.516 ± 0.022	1.325MetGly: 1.325 ± 0.042	0.32MetHis: 0.32 ± 0.015	0.773MetIle: 0.773 ± 0.026	0.361MetLys: 0.361 ± 0.018	1.836MetLeu: 1.836 ± 0.041	0.304MetMet: 0.304 ± 0.017	0.421MetAsn: 0.421 ± 0.017	1.175MetPro: 1.175 ± 0.033	0.521MetGln: 0.521 ± 0.021	1.464MetArg: 1.464 ± 0.04	1.441MetSer: 1.441 ± 0.033	1.659MetThr: 1.659 ± 0.036	1.28MetVal: 1.28 ± 0.032	0.261MetTrp: 0.261 ± 0.014	0.293MetTyr: 0.293 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.242AsnAla: 2.242 ± 0.048	0.096AsnCys: 0.096 ± 0.01	0.997AsnAsp: 0.997 ± 0.032	0.852AsnGlu: 0.852 ± 0.027	0.585AsnPhe: 0.585 ± 0.027	1.654AsnGly: 1.654 ± 0.041	0.31AsnHis: 0.31 ± 0.017	0.698AsnIle: 0.698 ± 0.028	0.294AsnLys: 0.294 ± 0.02	1.744AsnLeu: 1.744 ± 0.037	0.271AsnMet: 0.271 ± 0.018	0.384AsnAsn: 0.384 ± 0.021	1.495AsnPro: 1.495 ± 0.039	0.444AsnGln: 0.444 ± 0.019	1.152AsnArg: 1.152 ± 0.034	0.875AsnSer: 0.875 ± 0.031	0.989AsnThr: 0.989 ± 0.033	1.499AsnVal: 1.499 ± 0.041	0.28AsnTrp: 0.28 ± 0.016	0.433AsnTyr: 0.433 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	8.388ProAla: 8.388 ± 0.12	0.198ProCys: 0.198 ± 0.012	4.297ProAsp: 4.297 ± 0.063	4.045ProGlu: 4.045 ± 0.053	1.738ProPhe: 1.738 ± 0.038	5.54ProGly: 5.54 ± 0.074	1.246ProHis: 1.246 ± 0.039	1.913ProIle: 1.913 ± 0.044	0.741ProLys: 0.741 ± 0.029	5.194ProLeu: 5.194 ± 0.077	0.873ProMet: 0.873 ± 0.026	0.885ProAsn: 0.885 ± 0.026	2.932ProPro: 2.932 ± 0.071	1.538ProGln: 1.538 ± 0.043	3.89ProArg: 3.89 ± 0.061	3.088ProSer: 3.088 ± 0.051	3.339ProThr: 3.339 ± 0.06	5.197ProVal: 5.197 ± 0.069	0.907ProTrp: 0.907 ± 0.032	1.053ProTyr: 1.053 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.448GlnAla: 3.448 ± 0.052	0.111GlnCys: 0.111 ± 0.011	1.252GlnAsp: 1.252 ± 0.031	1.334GlnGlu: 1.334 ± 0.034	0.825GlnPhe: 0.825 ± 0.027	1.998GlnGly: 1.998 ± 0.044	0.61GlnHis: 0.61 ± 0.023	1.242GlnIle: 1.242 ± 0.028	0.481GlnLys: 0.481 ± 0.022	2.979GlnLeu: 2.979 ± 0.057	0.49GlnMet: 0.49 ± 0.022	0.583GlnAsn: 0.583 ± 0.024	1.561GlnPro: 1.561 ± 0.039	1.036GlnGln: 1.036 ± 0.033	2.506GlnArg: 2.506 ± 0.05	1.311GlnSer: 1.311 ± 0.036	1.401GlnThr: 1.401 ± 0.037	2.215GlnVal: 2.215 ± 0.042	0.546GlnTrp: 0.546 ± 0.02	0.567GlnTyr: 0.567 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	10.102ArgAla: 10.102 ± 0.13	0.341ArgCys: 0.341 ± 0.019	4.498ArgAsp: 4.498 ± 0.063	4.666ArgGlu: 4.666 ± 0.069	2.52ArgPhe: 2.52 ± 0.05	5.967ArgGly: 5.967 ± 0.07	1.689ArgHis: 1.689 ± 0.046	3.757ArgIle: 3.757 ± 0.056	1.187ArgLys: 1.187 ± 0.03	7.668ArgLeu: 7.668 ± 0.107	1.885ArgMet: 1.885 ± 0.04	1.281ArgAsn: 1.281 ± 0.036	4.164ArgPro: 4.164 ± 0.07	1.985ArgGln: 1.985 ± 0.041	7.448ArgArg: 7.448 ± 0.112	4.213ArgSer: 4.213 ± 0.066	4.656ArgThr: 4.656 ± 0.066	6.663ArgVal: 6.663 ± 0.089	1.287ArgTrp: 1.287 ± 0.037	1.611ArgTyr: 1.611 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	7.312SerAla: 7.312 ± 0.079	0.244SerCys: 0.244 ± 0.014	3.09SerAsp: 3.09 ± 0.047	2.534SerGlu: 2.534 ± 0.05	1.832SerPhe: 1.832 ± 0.038	5.662SerGly: 5.662 ± 0.079	1.003SerHis: 1.003 ± 0.032	2.294SerIle: 2.294 ± 0.048	0.789SerLys: 0.789 ± 0.03	5.223SerLeu: 5.223 ± 0.068	1.079SerMet: 1.079 ± 0.026	0.854SerAsn: 0.854 ± 0.027	3.265SerPro: 3.265 ± 0.059	1.2SerGln: 1.2 ± 0.034	3.799SerArg: 3.799 ± 0.057	2.941SerSer: 2.941 ± 0.053	3.167SerThr: 3.167 ± 0.057	4.677SerVal: 4.677 ± 0.064	0.895SerTrp: 0.895 ± 0.028	1.07SerTyr: 1.07 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	8.116ThrAla: 8.116 ± 0.084	0.31ThrCys: 0.31 ± 0.017	3.445ThrAsp: 3.445 ± 0.052	2.89ThrGlu: 2.89 ± 0.045	1.776ThrPhe: 1.776 ± 0.038	5.678ThrGly: 5.678 ± 0.075	1.137ThrHis: 1.137 ± 0.037	2.567ThrIle: 2.567 ± 0.058	0.889ThrLys: 0.889 ± 0.029	5.912ThrLeu: 5.912 ± 0.083	0.93ThrMet: 0.93 ± 0.032	1.0ThrAsn: 1.0 ± 0.032	3.973ThrPro: 3.973 ± 0.064	1.411ThrGln: 1.411 ± 0.034	3.993ThrArg: 3.993 ± 0.064	3.071ThrSer: 3.071 ± 0.055	3.551ThrThr: 3.551 ± 0.061	5.835ThrVal: 5.835 ± 0.078	0.881ThrTrp: 0.881 ± 0.029	1.16ThrTyr: 1.16 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	12.68ValAla: 12.68 ± 0.123	0.492ValCys: 0.492 ± 0.02	5.782ValAsp: 5.782 ± 0.067	4.833ValGlu: 4.833 ± 0.069	2.931ValPhe: 2.931 ± 0.055	7.263ValGly: 7.263 ± 0.087	1.813ValHis: 1.813 ± 0.038	4.118ValIle: 4.118 ± 0.068	1.286ValLys: 1.286 ± 0.042	9.937ValLeu: 9.937 ± 0.104	1.402ValMet: 1.402 ± 0.032	1.708ValAsn: 1.708 ± 0.041	5.071ValPro: 5.071 ± 0.061	2.309ValGln: 2.309 ± 0.043	6.992ValArg: 6.992 ± 0.092	4.981ValSer: 4.981 ± 0.069	5.742ValThr: 5.742 ± 0.077	9.39ValVal: 9.39 ± 0.111	1.333ValTrp: 1.333 ± 0.037	1.542ValTyr: 1.542 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.7TrpAla: 1.7 ± 0.044	0.111TrpCys: 0.111 ± 0.01	0.841TrpAsp: 0.841 ± 0.029	0.72TrpGlu: 0.72 ± 0.023	0.59TrpPhe: 0.59 ± 0.022	1.183TrpGly: 1.183 ± 0.03	0.366TrpHis: 0.366 ± 0.017	0.734TrpIle: 0.734 ± 0.029	0.249TrpLys: 0.249 ± 0.016	1.756TrpLeu: 1.756 ± 0.043	0.38TrpMet: 0.38 ± 0.019	0.427TrpAsn: 0.427 ± 0.023	0.797TrpPro: 0.797 ± 0.027	0.558TrpGln: 0.558 ± 0.023	1.392TrpArg: 1.392 ± 0.039	0.957TrpSer: 0.957 ± 0.025	0.969TrpThr: 0.969 ± 0.032	1.217TrpVal: 1.217 ± 0.037	0.377TrpTrp: 0.377 ± 0.021	0.32TrpTyr: 0.32 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.574TyrAla: 2.574 ± 0.055	0.12TyrCys: 0.12 ± 0.01	1.313TyrAsp: 1.313 ± 0.034	1.083TyrGlu: 1.083 ± 0.037	0.69TyrPhe: 0.69 ± 0.028	1.893TyrGly: 1.893 ± 0.036	0.31TyrHis: 0.31 ± 0.016	0.694TyrIle: 0.694 ± 0.026	0.275TyrLys: 0.275 ± 0.016	2.099TyrLeu: 2.099 ± 0.042	0.245TyrMet: 0.245 ± 0.014	0.408TyrAsn: 0.408 ± 0.02	1.053TyrPro: 1.053 ± 0.032	0.512TyrGln: 0.512 ± 0.019	1.719TyrArg: 1.719 ± 0.037	1.005TyrSer: 1.005 ± 0.034	1.123TyrThr: 1.123 ± 0.03	1.653TyrVal: 1.653 ± 0.039	0.309TyrTrp: 0.309 ± 0.016	0.472TyrTyr: 0.472 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3585 proteins (1157956 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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