Streptomyces phage Moab

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gilsonvirus; unclassified Gilsonvirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 233 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G9LDA3|A0A6G9LDA3_9CAUD CxxC_CXXC_SSSS domain-containing protein OS=Streptomyces phage Moab OX=2719210 GN=84 PE=4 SV=1
MM1 pKa = 7.7SDD3 pKa = 4.53YY4 pKa = 10.11MDD6 pKa = 5.78CDD8 pKa = 3.19ACDD11 pKa = 3.22WVDD14 pKa = 3.36IGVGMQMVRR23 pKa = 11.84PDD25 pKa = 3.14HH26 pKa = 5.87FCRR29 pKa = 11.84VKK31 pKa = 10.47AHH33 pKa = 7.16RR34 pKa = 11.84IDD36 pKa = 3.5AYY38 pKa = 10.68AYY40 pKa = 8.2YY41 pKa = 10.59DD42 pKa = 3.62EE43 pKa = 6.03LRR45 pKa = 11.84GQEE48 pKa = 4.61LIDD51 pKa = 3.51AVEE54 pKa = 4.26EE55 pKa = 3.9NWPPFWDD62 pKa = 3.84EE63 pKa = 3.88SLEE66 pKa = 4.35SIEE69 pKa = 4.31

Molecular weight: 8.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G9LBG8|A0A6G9LBG8_9CAUD RecA-like DNA recombinase OS=Streptomyces phage Moab OX=2719210 GN=65 PE=3 SV=1
MM1 pKa = 7.14FTPEE5 pKa = 3.75YY6 pKa = 9.99KK7 pKa = 10.31KK8 pKa = 10.96YY9 pKa = 10.36IEE11 pKa = 3.94SDD13 pKa = 2.72RR14 pKa = 11.84WKK16 pKa = 10.49LVCARR21 pKa = 11.84YY22 pKa = 7.95WATYY26 pKa = 8.65GRR28 pKa = 11.84KK29 pKa = 8.89CQACGSRR36 pKa = 11.84KK37 pKa = 9.31NLHH40 pKa = 5.16VHH42 pKa = 6.02HH43 pKa = 6.37NTYY46 pKa = 10.58EE47 pKa = 4.11RR48 pKa = 11.84FGRR51 pKa = 11.84EE52 pKa = 3.54ILSDD56 pKa = 3.64LTGVCQPCHH65 pKa = 6.01RR66 pKa = 11.84AIHH69 pKa = 6.46AIHH72 pKa = 6.5RR73 pKa = 11.84RR74 pKa = 11.84DD75 pKa = 3.24RR76 pKa = 11.84RR77 pKa = 11.84KK78 pKa = 10.07SLKK81 pKa = 10.51LVTLSFVKK89 pKa = 10.57AKK91 pKa = 10.42RR92 pKa = 11.84RR93 pKa = 11.84LL94 pKa = 3.43

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

233

0

233

37032

29

2113

158.9

17.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.047 ± 0.447

1.08 ± 0.123

6.484 ± 0.171

6.756 ± 0.303

3.867 ± 0.139

7.974 ± 0.225

1.844 ± 0.147

5.341 ± 0.15

6.07 ± 0.232

6.932 ± 0.18

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.803 ± 0.106

4.709 ± 0.17

3.624 ± 0.159

3.165 ± 0.202

5.487 ± 0.188

6.173 ± 0.243

6.278 ± 0.341

7.364 ± 0.218

1.917 ± 0.108

4.083 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski