Bacillus anthracis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes;

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5493 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H3A920|A0A6H3A920_BACAN Cation-transporting ATPase E1-E2 family OS=Bacillus anthracis OX=1392 GN=GBAA_0405 PE=4 SV=1
MM1 pKa = 8.17DD2 pKa = 3.89KK3 pKa = 10.79QKK5 pKa = 11.1AIDD8 pKa = 4.26LLNSLEE14 pKa = 4.19IYY16 pKa = 10.5DD17 pKa = 4.03YY18 pKa = 11.58DD19 pKa = 4.87ADD21 pKa = 4.94GEE23 pKa = 4.25ILYY26 pKa = 10.39YY27 pKa = 11.16ALVKK31 pKa = 10.85LNEE34 pKa = 4.33DD35 pKa = 3.45SKK37 pKa = 11.73KK38 pKa = 10.78VIEE41 pKa = 4.26SLLPEE46 pKa = 3.79GVNFNEE52 pKa = 4.21GLDD55 pKa = 3.92DD56 pKa = 4.37KK57 pKa = 12.0GEE59 pKa = 4.23LFDD62 pKa = 3.69ITLFCWEE69 pKa = 3.92YY70 pKa = 11.66AEE72 pKa = 4.72WFNGDD77 pKa = 2.73QFMAEE82 pKa = 4.27EE83 pKa = 4.3PKK85 pKa = 10.59EE86 pKa = 4.34VYY88 pKa = 10.37CEE90 pKa = 4.3SNN92 pKa = 2.86

Molecular weight: 10.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q81KZ0|PFKA_BACAN ATP-dependent 6-phosphofructokinase OS=Bacillus anthracis OX=1392 GN=pfkA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5490

3

5493

1439306

28

5017

262.0

29.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.695 ± 0.032

0.848 ± 0.011

4.658 ± 0.024

7.568 ± 0.05

4.69 ± 0.031

6.654 ± 0.035

2.122 ± 0.019

8.073 ± 0.041

7.465 ± 0.044

9.505 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.865 ± 0.017

4.621 ± 0.037

3.379 ± 0.025

3.701 ± 0.022

3.764 ± 0.028

5.794 ± 0.026

5.574 ± 0.047

7.252 ± 0.038

1.026 ± 0.013

3.747 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski