Clostridium disporicum
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3202 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A174DQU7|A0A174DQU7_9CLOT MobA-like protein OS=Clostridium disporicum OX=84024 GN=ERS852471_01255 PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.41 VNEE5 pKa = 4.42 IVSFLQEE12 pKa = 3.95 NEE14 pKa = 4.23 LGDD17 pKa = 3.98 VEE19 pKa = 4.26 ILKK22 pKa = 10.31 SEE24 pKa = 4.1 KK25 pKa = 10.53 DD26 pKa = 3.34 LLLVNFYY33 pKa = 11.43 YY34 pKa = 10.87 DD35 pKa = 3.51 FDD37 pKa = 4.65 EE38 pKa = 5.72 DD39 pKa = 4.02 LLAAAKK45 pKa = 10.11 AYY47 pKa = 10.33 AKK49 pKa = 10.01 EE50 pKa = 4.07 EE51 pKa = 4.37 CEE53 pKa = 4.7 DD54 pKa = 3.77 EE55 pKa = 5.63 SSEE58 pKa = 3.99 AWEE61 pKa = 4.02 KK62 pKa = 10.94 EE63 pKa = 3.87 YY64 pKa = 11.09 YY65 pKa = 10.32 LPYY68 pKa = 10.63 LYY70 pKa = 10.35 DD71 pKa = 3.5 FCNDD75 pKa = 3.06 EE76 pKa = 4.14 VLSIVEE82 pKa = 5.18 DD83 pKa = 3.21 ITEE86 pKa = 4.06 EE87 pKa = 3.98 LNIAGEE93 pKa = 4.25 FMAFQMKK100 pKa = 10.31 DD101 pKa = 2.7 RR102 pKa = 11.84 TAEE105 pKa = 3.94 YY106 pKa = 9.41 VQFMALFTNEE116 pKa = 4.7 DD117 pKa = 3.18 SDD119 pKa = 3.81 ISIEE123 pKa = 4.23 EE124 pKa = 4.04 VVKK127 pKa = 10.71 DD128 pKa = 3.8 YY129 pKa = 11.24 IGG131 pKa = 3.41
Molecular weight: 15.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.77
IPC_protein 3.719
Toseland 3.541
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.579
Rodwell 3.554
Grimsley 3.452
Solomon 3.668
Lehninger 3.63
Nozaki 3.808
DTASelect 3.948
Thurlkill 3.567
EMBOSS 3.592
Sillero 3.834
Patrickios 0.985
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.764
Protein with the highest isoelectric point:
>tr|A0A174IRU3|A0A174IRU3_9CLOT 50S ribosomal protein L13 OS=Clostridium disporicum OX=84024 GN=rplM PE=3 SV=1
MM1 pKa = 7.29 ARR3 pKa = 11.84 KK4 pKa = 9.68 AIIEE8 pKa = 4.24 KK9 pKa = 9.41 WSKK12 pKa = 7.47 TPKK15 pKa = 8.19 YY16 pKa = 5.65 TTRR19 pKa = 11.84 AYY21 pKa = 8.63 TRR23 pKa = 11.84 CRR25 pKa = 11.84 ICGRR29 pKa = 11.84 PHH31 pKa = 6.07 AVLRR35 pKa = 11.84 KK36 pKa = 9.58 FGICRR41 pKa = 11.84 ICFRR45 pKa = 11.84 EE46 pKa = 3.81 LAYY49 pKa = 10.04 KK50 pKa = 10.84 GEE52 pKa = 4.18 IPGCRR57 pKa = 11.84 KK58 pKa = 10.18 ASWW61 pKa = 3.01
Molecular weight: 7.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 9.663
IPC_protein 10.131
Toseland 10.57
ProMoST 10.189
Dawson 10.687
Bjellqvist 10.365
Wikipedia 10.847
Rodwell 11.067
Grimsley 10.73
Solomon 10.745
Lehninger 10.73
Nozaki 10.599
DTASelect 10.35
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.613
Patrickios 10.818
IPC_peptide 10.76
IPC2_peptide 9.604
IPC2.peptide.svr19 8.467
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3202
0
3202
981246
30
2283
306.4
34.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.659 ± 0.051
1.192 ± 0.019
5.724 ± 0.041
7.612 ± 0.046
4.386 ± 0.035
6.574 ± 0.044
1.274 ± 0.015
10.062 ± 0.058
8.587 ± 0.044
9.009 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.601 ± 0.02
6.384 ± 0.048
2.711 ± 0.021
2.08 ± 0.019
3.396 ± 0.029
6.162 ± 0.031
4.956 ± 0.035
6.677 ± 0.04
0.678 ± 0.012
4.274 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here