Taro bacilliform CH virus
Average proteome isoelectric point is 7.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E3LZB2|A0A0E3LZB2_9VIRU Uncharacterized protein OS=Taro bacilliform CH virus OX=1634914 PE=4 SV=1
MM1 pKa = 7.46 EE2 pKa = 4.83 NAADD6 pKa = 4.08 NYY8 pKa = 10.73 CKK10 pKa = 9.98 ILKK13 pKa = 10.01 EE14 pKa = 3.97 MAQLPKK20 pKa = 10.68 PMTLLHH26 pKa = 5.73 EE27 pKa = 4.53 MSRR30 pKa = 11.84 EE31 pKa = 3.78 ALLEE35 pKa = 3.86 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 10.16 VQSQLTLALQEE49 pKa = 4.32 MEE51 pKa = 4.18 QCLLAQYY58 pKa = 10.97 SYY60 pKa = 11.2 FSQHH64 pKa = 5.11 ATKK67 pKa = 10.71 DD68 pKa = 3.96 NYY70 pKa = 10.21 WGDD73 pKa = 3.66 HH74 pKa = 5.67 LQDD77 pKa = 3.67 LQEE80 pKa = 4.06 QMEE83 pKa = 4.64 AFHH86 pKa = 7.1 KK87 pKa = 9.6 LTISIMEE94 pKa = 4.42 CTLHH98 pKa = 5.95 TAHH101 pKa = 7.41 PP102 pKa = 4.13
Molecular weight: 11.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.37
IPC2_protein 5.41
IPC_protein 5.372
Toseland 5.728
ProMoST 5.664
Dawson 5.563
Bjellqvist 5.588
Wikipedia 5.537
Rodwell 5.537
Grimsley 5.855
Solomon 5.563
Lehninger 5.55
Nozaki 5.792
DTASelect 5.97
Thurlkill 5.944
EMBOSS 5.893
Sillero 5.893
Patrickios 3.605
IPC_peptide 5.575
IPC2_peptide 5.919
IPC2.peptide.svr19 5.893
Protein with the highest isoelectric point:
>tr|A0A0E3LXE8|A0A0E3LXE8_9VIRU Uncharacterized protein OS=Taro bacilliform CH virus OX=1634914 PE=4 SV=1
MM1 pKa = 6.4 QQKK4 pKa = 6.25 TTTGEE9 pKa = 3.78 TTYY12 pKa = 10.97 KK13 pKa = 10.17 IFRR16 pKa = 11.84 NRR18 pKa = 11.84 WRR20 pKa = 11.84 HH21 pKa = 4.06 STNSQLALWNAPYY34 pKa = 9.18 TQLTRR39 pKa = 11.84 EE40 pKa = 4.23 AMVGPLLKK48 pKa = 10.23 TMFGVGTKK56 pKa = 9.82 PYY58 pKa = 9.92 RR59 pKa = 11.84 AILAHH64 pKa = 6.61 HH65 pKa = 7.05 SGPLLSVEE73 pKa = 4.95 RR74 pKa = 11.84 IRR76 pKa = 11.84 FLKK79 pKa = 10.35 SYY81 pKa = 9.56 HH82 pKa = 5.88 FCCFLYY88 pKa = 9.75 TQTRR92 pKa = 11.84 DD93 pKa = 3.48 AITQRR98 pKa = 11.84 TMGPHH103 pKa = 6.23 AHH105 pKa = 7.11 PLL107 pKa = 3.47
Molecular weight: 12.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.882
IPC_protein 10.57
Toseland 10.423
ProMoST 10.189
Dawson 10.613
Bjellqvist 10.35
Wikipedia 10.833
Rodwell 10.877
Grimsley 10.701
Solomon 10.672
Lehninger 10.643
Nozaki 10.438
DTASelect 10.335
Thurlkill 10.467
EMBOSS 10.833
Sillero 10.54
Patrickios 10.599
IPC_peptide 10.672
IPC2_peptide 9.428
IPC2.peptide.svr19 8.365
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2402
102
1803
400.3
45.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.87 ± 0.67
1.79 ± 0.378
4.621 ± 1.108
7.619 ± 1.272
3.372 ± 0.489
5.329 ± 1.02
2.706 ± 0.683
6.869 ± 1.3
6.661 ± 0.906
8.868 ± 2.222
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.748 ± 1.08
4.704 ± 0.466
4.788 ± 0.386
5.704 ± 0.573
5.62 ± 0.554
6.661 ± 0.653
6.162 ± 1.484
4.663 ± 0.711
1.291 ± 0.196
3.955 ± 0.659
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here