Lycianthes yellow mosaic virus
Average proteome isoelectric point is 8.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140D6R5|A0A140D6R5_9GEMI Movement protein BC1 OS=Lycianthes yellow mosaic virus OX=1779714 GN=BC PE=3 SV=1
MM1 pKa = 7.33 EE2 pKa = 5.69 HH3 pKa = 6.59 NNNAVAYY10 pKa = 5.35 TTSDD14 pKa = 2.84 RR15 pKa = 11.84 TEE17 pKa = 3.9 FQLSNEE23 pKa = 4.1 KK24 pKa = 9.97 TEE26 pKa = 4.14 VTLLFPSTIDD36 pKa = 3.27 QKK38 pKa = 11.06 FSKK41 pKa = 10.79 LLGKK45 pKa = 9.26 CLRR48 pKa = 11.84 IDD50 pKa = 3.67 HH51 pKa = 6.78 VILEE55 pKa = 4.31 YY56 pKa = 10.8 RR57 pKa = 11.84 NQVPVNATGHH67 pKa = 5.14 VVIEE71 pKa = 4.01 MHH73 pKa = 6.5 DD74 pKa = 3.69 TRR76 pKa = 11.84 LHH78 pKa = 6.18 EE79 pKa = 4.92 GDD81 pKa = 3.64 SKK83 pKa = 10.99 QAEE86 pKa = 4.06 FTIPIGCNCNIHH98 pKa = 6.3 YY99 pKa = 10.23 YY100 pKa = 9.48 SSSYY104 pKa = 10.47 FSPKK108 pKa = 10.33 DD109 pKa = 3.49 PNPWRR114 pKa = 11.84 VMYY117 pKa = 10.43 RR118 pKa = 11.84 VDD120 pKa = 3.44 DD121 pKa = 4.19 TNVVNGVHH129 pKa = 6.6 FCRR132 pKa = 11.84 MQGKK136 pKa = 9.91 LKK138 pKa = 10.2 MSSAKK143 pKa = 9.73 QSSEE147 pKa = 3.7 IQFKK151 pKa = 10.45 SPKK154 pKa = 9.79 IEE156 pKa = 4.1 ILSKK160 pKa = 10.48 AYY162 pKa = 11.2 NMTNIDD168 pKa = 4.06 FWTVPHH174 pKa = 6.11 SQVSRR179 pKa = 11.84 KK180 pKa = 8.79 PVQALSNIRR189 pKa = 11.84 SQSCRR194 pKa = 11.84 YY195 pKa = 5.2 TTPAILPGQTWASASSVGNEE215 pKa = 4.06 DD216 pKa = 5.09 QEE218 pKa = 4.34 DD219 pKa = 3.71 LPYY222 pKa = 11.02 RR223 pKa = 11.84 NLHH226 pKa = 5.9 RR227 pKa = 11.84 LQDD230 pKa = 3.65 KK231 pKa = 8.97 TLDD234 pKa = 4.11 PGPSASEE241 pKa = 3.83 VVGNNQTVNDD251 pKa = 4.22 DD252 pKa = 3.87 VINIIKK258 pKa = 8.83 KK259 pKa = 7.36 TVEE262 pKa = 3.62 LCMEE266 pKa = 4.87 GSNVTSNVKK275 pKa = 9.77 PIKK278 pKa = 10.45
Molecular weight: 31.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.531
IPC2_protein 6.547
IPC_protein 6.693
Toseland 6.737
ProMoST 7.263
Dawson 7.205
Bjellqvist 7.102
Wikipedia 7.176
Rodwell 7.19
Grimsley 6.854
Solomon 7.249
Lehninger 7.249
Nozaki 7.454
DTASelect 7.497
Thurlkill 7.527
EMBOSS 7.556
Sillero 7.629
Patrickios 4.228
IPC_peptide 7.249
IPC2_peptide 7.102
IPC2.peptide.svr19 6.982
Protein with the highest isoelectric point:
>tr|A0A140D6R2|A0A140D6R2_9GEMI AC4 protein OS=Lycianthes yellow mosaic virus OX=1779714 GN=AC4 PE=3 SV=1
MM1 pKa = 7.38 AVDD4 pKa = 4.58 FRR6 pKa = 11.84 TGEE9 pKa = 4.38 YY10 pKa = 8.66 ITATQAQNGAYY21 pKa = 8.83 IWTVPNPLYY30 pKa = 10.81 FKK32 pKa = 10.36 VLKK35 pKa = 9.04 HH36 pKa = 5.45 TSRR39 pKa = 11.84 PFNSDD44 pKa = 2.24 HH45 pKa = 7.75 DD46 pKa = 4.12 YY47 pKa = 11.89 LEE49 pKa = 4.04 IQIQFNHH56 pKa = 5.87 NLRR59 pKa = 11.84 RR60 pKa = 11.84 ALGIHH65 pKa = 5.86 KK66 pKa = 9.88 CFLIFRR72 pKa = 11.84 IWTRR76 pKa = 11.84 LRR78 pKa = 11.84 PQTWRR83 pKa = 11.84 FLRR86 pKa = 11.84 VFKK89 pKa = 11.21 YY90 pKa = 10.66 NVIRR94 pKa = 11.84 FLNNLGVVSINNVIRR109 pKa = 11.84 SVNHH113 pKa = 5.24 VLWNVFDD120 pKa = 3.6 NTTYY124 pKa = 11.61 ARR126 pKa = 11.84 MLYY129 pKa = 10.06 AIKK132 pKa = 10.51 FNIYY136 pKa = 10.14
Molecular weight: 16.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 9.999
IPC_protein 10.701
Toseland 10.306
ProMoST 10.189
Dawson 10.555
Bjellqvist 10.321
Wikipedia 10.804
Rodwell 10.73
Grimsley 10.657
Solomon 10.613
Lehninger 10.555
Nozaki 10.306
DTASelect 10.321
Thurlkill 10.394
EMBOSS 10.73
Sillero 10.467
Patrickios 10.292
IPC_peptide 10.599
IPC2_peptide 9.341
IPC2.peptide.svr19 8.279
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
1798
85
362
199.8
22.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.505 ± 0.528
2.336 ± 0.337
4.672 ± 0.36
4.171 ± 0.551
4.616 ± 0.508
5.061 ± 0.636
3.782 ± 0.392
6.34 ± 0.687
5.562 ± 0.513
7.341 ± 0.71
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.225 ± 0.634
6.452 ± 0.6
5.006 ± 0.34
4.338 ± 0.543
6.952 ± 1.008
9.121 ± 0.79
5.228 ± 0.688
7.119 ± 0.788
1.168 ± 0.243
4.004 ± 0.617
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here