Gordonia phage Bizzy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vividuovirus; unclassified Vividuovirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M9F6|A0A3G3M9F6_9CAUD Exonuclease OS=Gordonia phage Bizzy OX=2483667 GN=48 PE=4 SV=1
MM1 pKa = 7.08SRR3 pKa = 11.84AVVFTAWASTVVEE16 pKa = 4.37VPDD19 pKa = 3.87DD20 pKa = 4.14VEE22 pKa = 5.73AEE24 pKa = 4.42TYY26 pKa = 11.12AEE28 pKa = 4.05LVEE31 pKa = 4.29WASEE35 pKa = 3.83NMPYY39 pKa = 10.37PSLCHH44 pKa = 5.01QCGHH48 pKa = 5.83EE49 pKa = 4.0MSVGDD54 pKa = 4.61FEE56 pKa = 5.18TDD58 pKa = 3.22TVITADD64 pKa = 3.22GTEE67 pKa = 3.98YY68 pKa = 11.07DD69 pKa = 3.94VATGKK74 pKa = 9.34PRR76 pKa = 11.84HH77 pKa = 5.73EE78 pKa = 4.27PP79 pKa = 3.27

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3M865|A0A3G3M865_9CAUD Head-to-tail adaptor OS=Gordonia phage Bizzy OX=2483667 GN=27 PE=4 SV=1
MM1 pKa = 7.28TGEE4 pKa = 4.34TFPGPRR10 pKa = 11.84AVTQNGMTHH19 pKa = 6.47CTTCHH24 pKa = 5.37QSGLAWLVAKK34 pKa = 10.27SGKK37 pKa = 10.02RR38 pKa = 11.84YY39 pKa = 9.35LATSEE44 pKa = 4.26RR45 pKa = 11.84DD46 pKa = 3.49RR47 pKa = 11.84SPHH50 pKa = 6.25RR51 pKa = 11.84PACEE55 pKa = 3.83RR56 pKa = 11.84RR57 pKa = 11.84WTANRR62 pKa = 11.84AARR65 pKa = 3.9

Molecular weight: 7.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

18348

27

1671

215.9

23.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.426 ± 0.448

0.839 ± 0.134

7.521 ± 0.417

5.477 ± 0.319

2.393 ± 0.169

8.846 ± 0.432

2.093 ± 0.225

4.327 ± 0.28

2.376 ± 0.205

7.543 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.202 ± 0.116

2.665 ± 0.162

6.377 ± 0.315

3.772 ± 0.175

7.467 ± 0.443

4.878 ± 0.278

6.578 ± 0.259

7.957 ± 0.253

2.104 ± 0.117

2.158 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski