Amino acid dipepetide frequency for Mycobacterium phage JacoRen57

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.378AlaAla: 11.378 ± 1.531
0.489AlaCys: 0.489 ± 0.18
5.444AlaAsp: 5.444 ± 0.585
5.628AlaGlu: 5.628 ± 0.594
3.67AlaPhe: 3.67 ± 0.515
7.647AlaGly: 7.647 ± 1.096
1.591AlaHis: 1.591 ± 0.311
5.995AlaIle: 5.995 ± 0.545
5.139AlaLys: 5.139 ± 0.573
8.442AlaLeu: 8.442 ± 0.647
1.713AlaMet: 1.713 ± 0.358
3.12AlaAsn: 3.12 ± 0.406
4.833AlaPro: 4.833 ± 0.653
3.426AlaGln: 3.426 ± 0.5
6.423AlaArg: 6.423 ± 0.737
4.649AlaSer: 4.649 ± 0.574
5.016AlaThr: 5.016 ± 0.647
5.995AlaVal: 5.995 ± 0.536
2.386AlaTrp: 2.386 ± 0.357
1.896AlaTyr: 1.896 ± 0.39
0.0AlaXaa: 0.0 ± 0.0
Cys
0.367CysAla: 0.367 ± 0.152
0.184CysCys: 0.184 ± 0.127
0.795CysAsp: 0.795 ± 0.213
0.551CysGlu: 0.551 ± 0.185
0.184CysPhe: 0.184 ± 0.103
0.918CysGly: 0.918 ± 0.272
0.367CysHis: 0.367 ± 0.189
0.551CysIle: 0.551 ± 0.183
0.551CysLys: 0.551 ± 0.176
0.428CysLeu: 0.428 ± 0.15
0.245CysMet: 0.245 ± 0.114
0.061CysAsn: 0.061 ± 0.059
0.306CysPro: 0.306 ± 0.156
0.122CysGln: 0.122 ± 0.086
0.551CysArg: 0.551 ± 0.157
0.673CysSer: 0.673 ± 0.254
0.367CysThr: 0.367 ± 0.152
0.306CysVal: 0.306 ± 0.189
0.122CysTrp: 0.122 ± 0.087
0.367CysTyr: 0.367 ± 0.145
0.0CysXaa: 0.0 ± 0.0
Asp
5.811AspAla: 5.811 ± 0.611
0.551AspCys: 0.551 ± 0.171
4.894AspAsp: 4.894 ± 0.686
5.383AspGlu: 5.383 ± 0.935
1.346AspPhe: 1.346 ± 0.267
5.811AspGly: 5.811 ± 0.555
1.285AspHis: 1.285 ± 0.222
2.447AspIle: 2.447 ± 0.361
2.325AspLys: 2.325 ± 0.338
5.934AspLeu: 5.934 ± 0.609
1.468AspMet: 1.468 ± 0.242
2.325AspAsn: 2.325 ± 0.398
5.689AspPro: 5.689 ± 0.658
2.141AspGln: 2.141 ± 0.388
3.181AspArg: 3.181 ± 0.43
3.365AspSer: 3.365 ± 0.448
3.732AspThr: 3.732 ± 0.392
4.037AspVal: 4.037 ± 0.58
1.591AspTrp: 1.591 ± 0.26
2.386AspTyr: 2.386 ± 0.404
0.0AspXaa: 0.0 ± 0.0
Glu
6.484GluAla: 6.484 ± 0.684
0.489GluCys: 0.489 ± 0.151
5.628GluAsp: 5.628 ± 0.711
5.383GluGlu: 5.383 ± 0.934
2.692GluPhe: 2.692 ± 0.429
3.487GluGly: 3.487 ± 0.339
1.162GluHis: 1.162 ± 0.294
3.181GluIle: 3.181 ± 0.452
2.019GluLys: 2.019 ± 0.377
8.075GluLeu: 8.075 ± 0.948
1.774GluMet: 1.774 ± 0.335
2.325GluAsn: 2.325 ± 0.367
2.263GluPro: 2.263 ± 0.417
2.508GluGln: 2.508 ± 0.442
5.016GluArg: 5.016 ± 0.734
2.386GluSer: 2.386 ± 0.361
3.487GluThr: 3.487 ± 0.509
3.303GluVal: 3.303 ± 0.62
1.407GluTrp: 1.407 ± 0.28
1.713GluTyr: 1.713 ± 0.328
0.0GluXaa: 0.0 ± 0.0
Phe
2.63PheAla: 2.63 ± 0.398
0.428PheCys: 0.428 ± 0.152
2.325PheAsp: 2.325 ± 0.431
1.468PheGlu: 1.468 ± 0.288
1.285PhePhe: 1.285 ± 0.313
3.365PheGly: 3.365 ± 0.581
0.551PheHis: 0.551 ± 0.208
1.652PheIle: 1.652 ± 0.323
1.529PheLys: 1.529 ± 0.292
2.692PheLeu: 2.692 ± 0.357
0.489PheMet: 0.489 ± 0.159
1.346PheAsn: 1.346 ± 0.334
2.08PhePro: 2.08 ± 0.344
1.346PheGln: 1.346 ± 0.24
2.325PheArg: 2.325 ± 0.365
2.263PheSer: 2.263 ± 0.364
1.958PheThr: 1.958 ± 0.275
2.325PheVal: 2.325 ± 0.41
0.612PheTrp: 0.612 ± 0.21
0.918PheTyr: 0.918 ± 0.297
0.0PheXaa: 0.0 ± 0.0
Gly
6.24GlyAla: 6.24 ± 0.751
0.734GlyCys: 0.734 ± 0.205
4.282GlyAsp: 4.282 ± 0.476
4.282GlyGlu: 4.282 ± 0.65
2.875GlyPhe: 2.875 ± 0.385
5.506GlyGly: 5.506 ± 0.746
1.223GlyHis: 1.223 ± 0.258
3.548GlyIle: 3.548 ± 0.546
4.404GlyLys: 4.404 ± 0.551
5.75GlyLeu: 5.75 ± 0.672
2.263GlyMet: 2.263 ± 0.328
3.12GlyAsn: 3.12 ± 0.531
4.16GlyPro: 4.16 ± 0.642
2.386GlyGln: 2.386 ± 0.41
5.077GlyArg: 5.077 ± 0.612
4.894GlySer: 4.894 ± 0.807
5.383GlyThr: 5.383 ± 0.588
6.79GlyVal: 6.79 ± 0.715
2.141GlyTrp: 2.141 ± 0.375
3.12GlyTyr: 3.12 ± 0.562
0.0GlyXaa: 0.0 ± 0.0
His
1.285HisAla: 1.285 ± 0.304
0.245HisCys: 0.245 ± 0.133
1.285HisAsp: 1.285 ± 0.301
1.346HisGlu: 1.346 ± 0.265
0.918HisPhe: 0.918 ± 0.287
0.979HisGly: 0.979 ± 0.219
0.306HisHis: 0.306 ± 0.178
1.468HisIle: 1.468 ± 0.308
0.918HisLys: 0.918 ± 0.249
1.529HisLeu: 1.529 ± 0.444
0.551HisMet: 0.551 ± 0.185
0.979HisAsn: 0.979 ± 0.271
1.346HisPro: 1.346 ± 0.4
0.551HisGln: 0.551 ± 0.175
1.346HisArg: 1.346 ± 0.303
1.285HisSer: 1.285 ± 0.227
0.979HisThr: 0.979 ± 0.254
0.918HisVal: 0.918 ± 0.246
0.612HisTrp: 0.612 ± 0.242
0.551HisTyr: 0.551 ± 0.171
0.0HisXaa: 0.0 ± 0.0
Ile
5.444IleAla: 5.444 ± 0.631
0.245IleCys: 0.245 ± 0.119
4.16IleAsp: 4.16 ± 0.557
3.976IleGlu: 3.976 ± 0.385
1.285IlePhe: 1.285 ± 0.25
4.037IleGly: 4.037 ± 0.597
0.795IleHis: 0.795 ± 0.234
2.508IleIle: 2.508 ± 0.545
3.181IleLys: 3.181 ± 0.496
3.487IleLeu: 3.487 ± 0.429
1.101IleMet: 1.101 ± 0.263
1.591IleAsn: 1.591 ± 0.307
2.692IlePro: 2.692 ± 0.392
2.019IleGln: 2.019 ± 0.249
2.997IleArg: 2.997 ± 0.401
1.835IleSer: 1.835 ± 0.26
3.303IleThr: 3.303 ± 0.495
2.936IleVal: 2.936 ± 0.363
1.101IleTrp: 1.101 ± 0.224
1.652IleTyr: 1.652 ± 0.365
0.0IleXaa: 0.0 ± 0.0
Lys
5.506LysAla: 5.506 ± 0.739
0.367LysCys: 0.367 ± 0.169
2.997LysAsp: 2.997 ± 0.442
4.16LysGlu: 4.16 ± 0.574
1.162LysPhe: 1.162 ± 0.266
2.875LysGly: 2.875 ± 0.429
1.285LysHis: 1.285 ± 0.337
2.019LysIle: 2.019 ± 0.321
4.037LysLys: 4.037 ± 0.804
2.753LysLeu: 2.753 ± 0.484
1.285LysMet: 1.285 ± 0.291
1.652LysAsn: 1.652 ± 0.331
2.508LysPro: 2.508 ± 0.376
2.508LysGln: 2.508 ± 0.461
3.732LysArg: 3.732 ± 0.569
2.508LysSer: 2.508 ± 0.464
2.997LysThr: 2.997 ± 0.524
4.282LysVal: 4.282 ± 0.469
1.346LysTrp: 1.346 ± 0.245
1.591LysTyr: 1.591 ± 0.331
0.0LysXaa: 0.0 ± 0.0
Leu
6.484LeuAla: 6.484 ± 0.634
0.612LeuCys: 0.612 ± 0.184
5.016LeuAsp: 5.016 ± 0.562
5.567LeuGlu: 5.567 ± 0.622
2.386LeuPhe: 2.386 ± 0.425
5.506LeuGly: 5.506 ± 0.849
1.591LeuHis: 1.591 ± 0.292
4.466LeuIle: 4.466 ± 0.472
4.772LeuLys: 4.772 ± 0.677
6.179LeuLeu: 6.179 ± 0.585
1.652LeuMet: 1.652 ± 0.318
3.609LeuAsn: 3.609 ± 0.453
4.649LeuPro: 4.649 ± 0.434
3.12LeuGln: 3.12 ± 0.526
5.383LeuArg: 5.383 ± 0.569
4.772LeuSer: 4.772 ± 0.587
4.343LeuThr: 4.343 ± 0.513
4.772LeuVal: 4.772 ± 0.389
1.223LeuTrp: 1.223 ± 0.287
2.508LeuTyr: 2.508 ± 0.595
0.0LeuXaa: 0.0 ± 0.0
Met
2.692MetAla: 2.692 ± 0.39
0.122MetCys: 0.122 ± 0.084
1.285MetAsp: 1.285 ± 0.241
1.407MetGlu: 1.407 ± 0.266
0.673MetPhe: 0.673 ± 0.164
1.162MetGly: 1.162 ± 0.286
0.306MetHis: 0.306 ± 0.163
1.407MetIle: 1.407 ± 0.272
1.652MetLys: 1.652 ± 0.301
1.958MetLeu: 1.958 ± 0.333
0.856MetMet: 0.856 ± 0.255
1.223MetAsn: 1.223 ± 0.271
1.468MetPro: 1.468 ± 0.222
1.04MetGln: 1.04 ± 0.245
1.835MetArg: 1.835 ± 0.261
2.019MetSer: 2.019 ± 0.359
1.958MetThr: 1.958 ± 0.389
1.346MetVal: 1.346 ± 0.292
0.489MetTrp: 0.489 ± 0.168
0.612MetTyr: 0.612 ± 0.247
0.0MetXaa: 0.0 ± 0.0
Asn
5.016AsnAla: 5.016 ± 0.689
0.306AsnCys: 0.306 ± 0.137
2.508AsnAsp: 2.508 ± 0.353
2.63AsnGlu: 2.63 ± 0.449
1.468AsnPhe: 1.468 ± 0.478
3.059AsnGly: 3.059 ± 0.408
0.673AsnHis: 0.673 ± 0.22
1.407AsnIle: 1.407 ± 0.288
2.141AsnLys: 2.141 ± 0.357
2.447AsnLeu: 2.447 ± 0.379
0.856AsnMet: 0.856 ± 0.249
2.08AsnAsn: 2.08 ± 0.369
3.915AsnPro: 3.915 ± 0.674
1.285AsnGln: 1.285 ± 0.266
2.263AsnArg: 2.263 ± 0.412
1.713AsnSer: 1.713 ± 0.363
1.896AsnThr: 1.896 ± 0.458
2.386AsnVal: 2.386 ± 0.362
0.918AsnTrp: 0.918 ± 0.227
0.795AsnTyr: 0.795 ± 0.17
0.0AsnXaa: 0.0 ± 0.0
Pro
4.588ProAla: 4.588 ± 0.504
0.551ProCys: 0.551 ± 0.208
4.16ProAsp: 4.16 ± 0.674
4.343ProGlu: 4.343 ± 0.645
1.958ProPhe: 1.958 ± 0.328
6.179ProGly: 6.179 ± 0.571
1.285ProHis: 1.285 ± 0.303
2.875ProIle: 2.875 ± 0.493
2.692ProLys: 2.692 ± 0.655
3.976ProLeu: 3.976 ± 0.49
1.652ProMet: 1.652 ± 0.387
1.774ProAsn: 1.774 ± 0.329
3.487ProPro: 3.487 ± 0.552
1.958ProGln: 1.958 ± 0.4
3.059ProArg: 3.059 ± 0.516
2.875ProSer: 2.875 ± 0.479
4.221ProThr: 4.221 ± 0.498
3.732ProVal: 3.732 ± 0.424
1.652ProTrp: 1.652 ± 0.43
1.529ProTyr: 1.529 ± 0.275
0.0ProXaa: 0.0 ± 0.0
Gln
4.343GlnAla: 4.343 ± 0.706
0.245GlnCys: 0.245 ± 0.134
1.896GlnAsp: 1.896 ± 0.325
2.569GlnGlu: 2.569 ± 0.452
1.101GlnPhe: 1.101 ± 0.277
3.365GlnGly: 3.365 ± 0.438
0.612GlnHis: 0.612 ± 0.162
2.08GlnIle: 2.08 ± 0.363
1.713GlnLys: 1.713 ± 0.327
3.303GlnLeu: 3.303 ± 0.412
1.591GlnMet: 1.591 ± 0.3
1.835GlnAsn: 1.835 ± 0.364
2.325GlnPro: 2.325 ± 0.427
2.263GlnGln: 2.263 ± 0.457
2.692GlnArg: 2.692 ± 0.402
1.835GlnSer: 1.835 ± 0.342
1.468GlnThr: 1.468 ± 0.311
1.713GlnVal: 1.713 ± 0.264
0.856GlnTrp: 0.856 ± 0.231
0.979GlnTyr: 0.979 ± 0.295
0.0GlnXaa: 0.0 ± 0.0
Arg
5.077ArgAla: 5.077 ± 0.564
0.489ArgCys: 0.489 ± 0.161
3.12ArgAsp: 3.12 ± 0.417
3.732ArgGlu: 3.732 ± 0.485
2.202ArgPhe: 2.202 ± 0.368
4.71ArgGly: 4.71 ± 0.548
1.04ArgHis: 1.04 ± 0.277
2.814ArgIle: 2.814 ± 0.347
3.732ArgLys: 3.732 ± 0.617
5.383ArgLeu: 5.383 ± 0.642
1.958ArgMet: 1.958 ± 0.371
3.365ArgAsn: 3.365 ± 0.472
3.303ArgPro: 3.303 ± 0.69
2.875ArgGln: 2.875 ± 0.383
4.466ArgArg: 4.466 ± 0.802
2.997ArgSer: 2.997 ± 0.417
4.037ArgThr: 4.037 ± 0.657
4.833ArgVal: 4.833 ± 0.602
1.285ArgTrp: 1.285 ± 0.325
2.141ArgTyr: 2.141 ± 0.336
0.0ArgXaa: 0.0 ± 0.0
Ser
4.588SerAla: 4.588 ± 0.514
0.367SerCys: 0.367 ± 0.134
3.303SerAsp: 3.303 ± 0.407
2.569SerGlu: 2.569 ± 0.381
2.263SerPhe: 2.263 ± 0.424
5.689SerGly: 5.689 ± 0.779
1.223SerHis: 1.223 ± 0.29
2.447SerIle: 2.447 ± 0.368
2.141SerLys: 2.141 ± 0.435
3.609SerLeu: 3.609 ± 0.471
1.896SerMet: 1.896 ± 0.388
1.774SerAsn: 1.774 ± 0.263
2.753SerPro: 2.753 ± 0.413
2.325SerGln: 2.325 ± 0.436
3.793SerArg: 3.793 ± 0.47
4.037SerSer: 4.037 ± 0.56
2.997SerThr: 2.997 ± 0.447
3.12SerVal: 3.12 ± 0.377
1.101SerTrp: 1.101 ± 0.237
1.652SerTyr: 1.652 ± 0.283
0.0SerXaa: 0.0 ± 0.0
Thr
5.873ThrAla: 5.873 ± 0.725
0.367ThrCys: 0.367 ± 0.148
3.365ThrAsp: 3.365 ± 0.327
2.569ThrGlu: 2.569 ± 0.475
2.202ThrPhe: 2.202 ± 0.377
5.75ThrGly: 5.75 ± 0.564
1.162ThrHis: 1.162 ± 0.279
3.303ThrIle: 3.303 ± 0.387
2.386ThrLys: 2.386 ± 0.452
4.282ThrLeu: 4.282 ± 0.564
1.529ThrMet: 1.529 ± 0.287
2.141ThrAsn: 2.141 ± 0.498
4.221ThrPro: 4.221 ± 0.559
2.019ThrGln: 2.019 ± 0.434
3.548ThrArg: 3.548 ± 0.523
3.12ThrSer: 3.12 ± 0.541
3.915ThrThr: 3.915 ± 0.759
4.527ThrVal: 4.527 ± 0.641
1.407ThrTrp: 1.407 ± 0.3
1.285ThrTyr: 1.285 ± 0.273
0.0ThrXaa: 0.0 ± 0.0
Val
6.607ValAla: 6.607 ± 0.521
0.489ValCys: 0.489 ± 0.187
5.261ValAsp: 5.261 ± 0.739
4.343ValGlu: 4.343 ± 0.504
1.958ValPhe: 1.958 ± 0.291
5.077ValGly: 5.077 ± 0.619
1.529ValHis: 1.529 ± 0.382
3.548ValIle: 3.548 ± 0.456
2.997ValLys: 2.997 ± 0.585
4.221ValLeu: 4.221 ± 0.439
1.346ValMet: 1.346 ± 0.281
2.936ValAsn: 2.936 ± 0.502
4.099ValPro: 4.099 ± 0.552
2.263ValGln: 2.263 ± 0.392
3.609ValArg: 3.609 ± 0.489
3.426ValSer: 3.426 ± 0.48
4.221ValThr: 4.221 ± 0.672
4.343ValVal: 4.343 ± 0.436
0.979ValTrp: 0.979 ± 0.195
2.019ValTyr: 2.019 ± 0.329
0.0ValXaa: 0.0 ± 0.0
Trp
2.141TrpAla: 2.141 ± 0.356
0.306TrpCys: 0.306 ± 0.139
1.774TrpAsp: 1.774 ± 0.361
1.162TrpGlu: 1.162 ± 0.29
1.407TrpPhe: 1.407 ± 0.233
1.223TrpGly: 1.223 ± 0.325
0.612TrpHis: 0.612 ± 0.213
0.979TrpIle: 0.979 ± 0.222
1.407TrpLys: 1.407 ± 0.325
1.835TrpLeu: 1.835 ± 0.376
0.489TrpMet: 0.489 ± 0.174
0.795TrpAsn: 0.795 ± 0.209
0.979TrpPro: 0.979 ± 0.22
1.407TrpGln: 1.407 ± 0.267
0.918TrpArg: 0.918 ± 0.231
1.407TrpSer: 1.407 ± 0.333
1.223TrpThr: 1.223 ± 0.243
1.529TrpVal: 1.529 ± 0.249
0.367TrpTrp: 0.367 ± 0.175
0.306TrpTyr: 0.306 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.325TyrAla: 2.325 ± 0.421
0.428TyrCys: 0.428 ± 0.158
2.202TyrAsp: 2.202 ± 0.333
1.713TyrGlu: 1.713 ± 0.325
0.551TyrPhe: 0.551 ± 0.193
1.835TyrGly: 1.835 ± 0.395
0.795TyrHis: 0.795 ± 0.254
1.652TyrIle: 1.652 ± 0.274
1.835TyrLys: 1.835 ± 0.362
2.325TyrLeu: 2.325 ± 0.43
0.734TyrMet: 0.734 ± 0.216
1.713TyrAsn: 1.713 ± 0.328
1.468TyrPro: 1.468 ± 0.333
1.223TyrGln: 1.223 ± 0.294
1.407TyrArg: 1.407 ± 0.363
1.591TyrSer: 1.591 ± 0.344
1.468TyrThr: 1.468 ± 0.31
2.08TyrVal: 2.08 ± 0.344
0.673TyrTrp: 0.673 ± 0.217
0.612TyrTyr: 0.612 ± 0.223
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (16348 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski