Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22970 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H2NX31|H2NX31_PONAB Uncharacterized protein OS=Pongo abelii OX=9601 PE=3 SV=1
MM1 pKa = 7.64 CPPVSMRR8 pKa = 11.84 YY9 pKa = 8.57 EE10 pKa = 4.14 EE11 pKa = 4.76 EE12 pKa = 4.15 GMSYY16 pKa = 10.81 LYY18 pKa = 10.63 AFWMYY23 pKa = 10.71 QLQHH27 pKa = 6.52 GDD29 pKa = 3.28 QLSICFTCFKK39 pKa = 10.87 AAFLDD44 pKa = 4.78 FKK46 pKa = 11.33 DD47 pKa = 4.13 LLEE50 pKa = 5.61 SEE52 pKa = 5.11 DD53 pKa = 3.77 WEE55 pKa = 4.28 EE56 pKa = 4.96 DD57 pKa = 3.12 NWDD60 pKa = 4.0 PEE62 pKa = 4.56 LMEE65 pKa = 4.29 HH66 pKa = 6.39 TEE68 pKa = 4.12 AEE70 pKa = 4.19 SDD72 pKa = 3.71 QEE74 pKa = 4.4 GSSGMEE80 pKa = 4.05 LSWGQSPGQPVQEE93 pKa = 4.81 GSEE96 pKa = 3.95 AWGPGTPAAAPEE108 pKa = 4.27 GSEE111 pKa = 4.81 DD112 pKa = 4.46 AGLDD116 pKa = 3.31 PHH118 pKa = 7.12 FVPTEE123 pKa = 4.26 LGPQEE128 pKa = 4.62 AVPLGLGHH136 pKa = 7.26 EE137 pKa = 4.57 DD138 pKa = 3.94 ADD140 pKa = 4.04 WTQGLPWRR148 pKa = 11.84 FEE150 pKa = 3.93 GLLTCSHH157 pKa = 6.89 WPSFFPSS164 pKa = 3.08
Molecular weight: 18.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 3.846
IPC_protein 3.795
Toseland 3.617
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.63
Rodwell 3.63
Grimsley 3.528
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 3.999
Thurlkill 3.643
EMBOSS 3.656
Sillero 3.91
Patrickios 0.528
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|H2NL65|H2NL65_PONAB Uncharacterized protein OS=Pongo abelii OX=9601 PE=4 SV=2
TT1 pKa = 7.54 PSHH4 pKa = 6.31 SLTRR8 pKa = 11.84 VFPLPTVTLHH18 pKa = 5.99 TPSHH22 pKa = 6.2 SLTRR26 pKa = 11.84 TPLPAVTLHH35 pKa = 6.09 TPSHH39 pKa = 6.03 SLTRR43 pKa = 11.84 VFPLPAVTLHH53 pKa = 6.09 TPSHH57 pKa = 6.09 SLTRR61 pKa = 11.84 TFPLPAVTLHH71 pKa = 6.06 TPSHH75 pKa = 5.73 SVTRR79 pKa = 11.84 VFPLPTVTLHH89 pKa = 5.99 TPSHH93 pKa = 6.2 SLTRR97 pKa = 11.84 TPLPAVTLHH106 pKa = 6.09 TPSHH110 pKa = 6.03 SLTRR114 pKa = 11.84 VFPLPTVTLHH124 pKa = 5.99 TPSHH128 pKa = 6.26 SLTRR132 pKa = 11.84 TPSPASLTRR141 pKa = 11.84 TFPLPAVTLHH151 pKa = 6.09 TPSHH155 pKa = 6.09 SLTRR159 pKa = 11.84 TFPLPAVTLHH169 pKa = 6.09 TPSHH173 pKa = 6.09 SLTRR177 pKa = 11.84 TFPLPAVTLHH187 pKa = 6.09 TPSHH191 pKa = 6.09 SLTRR195 pKa = 11.84 TFPLPAVTLHH205 pKa = 6.04 TPSHH209 pKa = 6.23 GLTRR213 pKa = 11.84 TFPLPAVTLHH223 pKa = 6.04 TPSHH227 pKa = 6.23 GLTRR231 pKa = 11.84 TFPLPAVTLQTPSHH245 pKa = 6.3 GLTRR249 pKa = 11.84 TFPLPTVTLHH259 pKa = 5.99 TPSHH263 pKa = 6.2 SLTRR267 pKa = 11.84 TPLPAVTLHH276 pKa = 6.01 TPSS279 pKa = 3.33
Molecular weight: 30.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.403
IPC_protein 13.013
Toseland 13.173
ProMoST 13.671
Dawson 13.173
Bjellqvist 13.173
Wikipedia 13.656
Rodwell 12.676
Grimsley 13.217
Solomon 13.671
Lehninger 13.583
Nozaki 13.173
DTASelect 13.173
Thurlkill 13.173
EMBOSS 13.671
Sillero 13.173
Patrickios 12.398
IPC_peptide 13.685
IPC2_peptide 12.676
IPC2.peptide.svr19 9.32
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21992
978
22970
11531378
7
31962
502.0
55.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.955 ± 0.018
2.28 ± 0.015
4.77 ± 0.011
7.103 ± 0.022
3.684 ± 0.012
6.543 ± 0.025
2.638 ± 0.01
4.398 ± 0.016
5.808 ± 0.021
9.94 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.163 ± 0.008
3.619 ± 0.012
6.206 ± 0.025
4.759 ± 0.018
5.654 ± 0.016
8.264 ± 0.021
5.291 ± 0.016
6.0 ± 0.015
1.226 ± 0.006
2.673 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here