Vibrio phage 1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H8YJE5|H8YJE5_9CAUD VHS1125 protein OS=Vibrio phage 1 OX=187944 PE=4 SV=1
MM1 pKa = 7.62FFVQPVNYY9 pKa = 9.82LNTEE13 pKa = 3.96GGVSYY18 pKa = 10.82EE19 pKa = 3.85AMTAEE24 pKa = 5.35FGDD27 pKa = 4.27GYY29 pKa = 11.56NRR31 pKa = 11.84INTTLFEE38 pKa = 4.93DD39 pKa = 3.51KK40 pKa = 10.98VGILIYY46 pKa = 10.62RR47 pKa = 11.84NTDD50 pKa = 3.34LEE52 pKa = 4.24QGVALDD58 pKa = 3.68VAKK61 pKa = 10.75GDD63 pKa = 3.78TVPNPKK69 pKa = 9.85PGEE72 pKa = 3.8NAIALNFHH80 pKa = 6.24TLQSIDD86 pKa = 3.48AVIGRR91 pKa = 11.84LQYY94 pKa = 9.64IRR96 pKa = 11.84EE97 pKa = 4.15VFVEE101 pKa = 4.09RR102 pKa = 11.84EE103 pKa = 3.61EE104 pKa = 4.02AAKK107 pKa = 10.38KK108 pKa = 10.8GKK110 pKa = 10.23DD111 pKa = 3.32PEE113 pKa = 6.35AGDD116 pKa = 3.96DD117 pKa = 5.44CPTEE121 pKa = 4.38DD122 pKa = 4.78CEE124 pKa = 4.86GTLEE128 pKa = 5.26HH129 pKa = 6.71YY130 pKa = 10.72PEE132 pKa = 4.92DD133 pKa = 3.87CRR135 pKa = 11.84CAVVPPPCTACTDD148 pKa = 3.75APLSCCVCGWSEE160 pKa = 3.97EE161 pKa = 4.22DD162 pKa = 3.55GQGVII167 pKa = 5.21

Molecular weight: 18.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H8YJE3|H8YJE3_9CAUD VHS1123 protein OS=Vibrio phage 1 OX=187944 PE=4 SV=1
MM1 pKa = 7.72CNTRR5 pKa = 11.84RR6 pKa = 11.84YY7 pKa = 10.43SPTVSDD13 pKa = 3.2KK14 pKa = 11.32RR15 pKa = 11.84GFNGEE20 pKa = 3.78AMEE23 pKa = 4.03QRR25 pKa = 11.84KK26 pKa = 9.38NGNYY30 pKa = 9.94VLYY33 pKa = 7.45TTHH36 pKa = 6.91ANVVNSLQRR45 pKa = 11.84RR46 pKa = 11.84IRR48 pKa = 11.84ALEE51 pKa = 4.04KK52 pKa = 10.66KK53 pKa = 9.74LANATKK59 pKa = 10.2KK60 pKa = 8.48QQ61 pKa = 3.3

Molecular weight: 7.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

125

0

125

24775

37

1379

198.2

22.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.827 ± 0.291

1.239 ± 0.113

6.046 ± 0.148

7.096 ± 0.227

3.637 ± 0.137

6.26 ± 0.178

1.974 ± 0.133

5.909 ± 0.195

6.62 ± 0.285

9.118 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.099 ± 0.115

5.296 ± 0.155

3.943 ± 0.18

4.19 ± 0.235

5.473 ± 0.169

5.61 ± 0.189

6.216 ± 0.201

6.49 ± 0.216

0.795 ± 0.069

3.16 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski