Kwoniella dejecticola CBS 10117
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8600 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1A5ZXR9|A0A1A5ZXR9_9TREE Uncharacterized protein OS=Kwoniella dejecticola CBS 10117 OX=1296121 GN=I303_07364 PE=4 SV=1
MM1 pKa = 7.72 KK2 pKa = 10.21 PRR4 pKa = 11.84 DD5 pKa = 3.91 SPVADD10 pKa = 3.61 DD11 pKa = 3.68 TFGGIEE17 pKa = 4.11 VFDD20 pKa = 4.14 AAGDD24 pKa = 3.83 SMGYY28 pKa = 9.39 ISKK31 pKa = 10.35 NLDD34 pKa = 3.06 EE35 pKa = 5.18 GGYY38 pKa = 9.15 TITTDD43 pKa = 3.09 PTQAVSISFTQDD55 pKa = 2.08 GSNPFSITISSNDD68 pKa = 3.25 RR69 pKa = 11.84 QAGYY73 pKa = 9.06 PYY75 pKa = 10.86 LGATLNTGSDD85 pKa = 3.44 MGVSSAAFANLGGISHH101 pKa = 7.65 IISGPSEE108 pKa = 4.11 SDD110 pKa = 3.12 DD111 pKa = 3.33 TGEE114 pKa = 4.2 SNTRR118 pKa = 11.84 QLYY121 pKa = 10.37 AGSEE125 pKa = 4.25 TAIFLYY131 pKa = 10.87 NADD134 pKa = 3.73 TNAITGQWTNTDD146 pKa = 3.1 NTKK149 pKa = 10.39 VDD151 pKa = 3.84 TIFYY155 pKa = 9.33 FGPKK159 pKa = 9.64 DD160 pKa = 3.67 SYY162 pKa = 10.83 SLGMIAPEE170 pKa = 4.73 NYY172 pKa = 9.91 DD173 pKa = 2.97 QFAIDD178 pKa = 4.81 FPEE181 pKa = 4.48 DD182 pKa = 3.49 QQKK185 pKa = 7.81 VTFKK189 pKa = 10.65 YY190 pKa = 10.96 VSIDD194 pKa = 3.35 PTPGVV199 pKa = 3.54
Molecular weight: 21.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.541
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.439
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.897
Patrickios 1.901
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A1A5ZY65|A0A1A5ZY65_9TREE Multidrug transporter OS=Kwoniella dejecticola CBS 10117 OX=1296121 GN=I303_07516 PE=4 SV=1
MM1 pKa = 7.77 SDD3 pKa = 2.61 SGYY6 pKa = 10.87 QVMALSPEE14 pKa = 4.36 TSPQTPNITPSPPLRR29 pKa = 11.84 QLSMSVPLALSADD42 pKa = 3.76 GSSAIHH48 pKa = 6.8 ASPVPPPRR56 pKa = 11.84 DD57 pKa = 3.07 IRR59 pKa = 11.84 ALLSHH64 pKa = 6.89 SPPLTPRR71 pKa = 11.84 SCKK74 pKa = 10.22 SSSTSPDD81 pKa = 3.04 LKK83 pKa = 11.09 KK84 pKa = 10.46 LDD86 pKa = 3.88 KK87 pKa = 10.81 RR88 pKa = 11.84 STASPSNFSRR98 pKa = 11.84 NLPPLTRR105 pKa = 11.84 TPPILPRR112 pKa = 11.84 PPIGLSLAGPSGNGRR127 pKa = 11.84 GTGIGLDD134 pKa = 3.66 AFRR137 pKa = 11.84 LGSPPKK143 pKa = 10.0 QHH145 pKa = 6.51 PQPGTATSHH154 pKa = 5.14 HH155 pKa = 6.17 HH156 pKa = 5.96 TRR158 pKa = 11.84 SFWVPSSTRR167 pKa = 11.84 NDD169 pKa = 2.98 QRR171 pKa = 11.84 SQHH174 pKa = 5.37 QRR176 pKa = 11.84 LASLPSIHH184 pKa = 6.57 TGHH187 pKa = 6.97 RR188 pKa = 11.84 EE189 pKa = 3.6 KK190 pKa = 10.24 TVYY193 pKa = 8.37 RR194 pKa = 11.84 TPVCIASQRR203 pKa = 11.84 SFPMSTLTPVTQGEE217 pKa = 4.18 ISSTISGRR225 pKa = 11.84 EE226 pKa = 3.8 SAYY229 pKa = 10.18 QPPPHH234 pKa = 6.84 HH235 pKa = 6.75 RR236 pKa = 11.84 HH237 pKa = 5.08 QSLSYY242 pKa = 10.45 SSHH245 pKa = 5.94 TPLFQDD251 pKa = 3.31 EE252 pKa = 4.66 PVTHH256 pKa = 6.86 RR257 pKa = 11.84 RR258 pKa = 11.84 HH259 pKa = 6.11 PSSGDD264 pKa = 3.14 AVIRR268 pKa = 11.84 FASHH272 pKa = 6.81 PLHH275 pKa = 6.45 RR276 pKa = 11.84 PAWAAPPPPPAHH288 pKa = 7.02 AISIPGRR295 pKa = 11.84 TQTMARR301 pKa = 11.84 PRR303 pKa = 11.84 LQIHH307 pKa = 7.12 PYY309 pKa = 9.78 APPRR313 pKa = 11.84 MDD315 pKa = 3.05 PRR317 pKa = 11.84 YY318 pKa = 9.78 YY319 pKa = 10.5 SAGGLGMQTHH329 pKa = 5.96 THH331 pKa = 6.98 PGLAHH336 pKa = 6.78 GGHH339 pKa = 6.36 ILGYY343 pKa = 9.61 GRR345 pKa = 11.84 EE346 pKa = 4.13 IVFTSPSSGISPGSFKK362 pKa = 10.93 APRR365 pKa = 11.84 KK366 pKa = 9.75 RR367 pKa = 11.84 ADD369 pKa = 3.57 DD370 pKa = 3.7 SQLAILNDD378 pKa = 3.72 VFEE381 pKa = 4.43 KK382 pKa = 9.93 TAYY385 pKa = 9.72 PSTDD389 pKa = 3.02 EE390 pKa = 4.2 RR391 pKa = 11.84 DD392 pKa = 3.29 EE393 pKa = 4.18 LARR396 pKa = 11.84 KK397 pKa = 9.91 LGMTSRR403 pKa = 11.84 SVQIWFQNRR412 pKa = 11.84 RR413 pKa = 11.84 RR414 pKa = 11.84 AVKK417 pKa = 10.22 VDD419 pKa = 3.18 AQSAVQRR426 pKa = 11.84 AEE428 pKa = 4.31 AEE430 pKa = 3.88 VDD432 pKa = 3.45 TQLMIRR438 pKa = 11.84 GPAPNIPRR446 pKa = 11.84 PYY448 pKa = 9.22 PVHH451 pKa = 5.37 TRR453 pKa = 11.84 HH454 pKa = 6.39 RR455 pKa = 11.84 SEE457 pKa = 4.16 SQEE460 pKa = 4.03 EE461 pKa = 4.71 VPLQPIVSAIDD472 pKa = 3.56 LSKK475 pKa = 10.99 EE476 pKa = 3.69 RR477 pKa = 11.84 VFALFPTTRR486 pKa = 11.84 PPLVASQSDD495 pKa = 3.66 MVMVKK500 pKa = 10.28 SEE502 pKa = 3.89 VMTPP506 pKa = 3.18
Molecular weight: 55.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.663
IPC_protein 10.628
Toseland 10.643
ProMoST 10.452
Dawson 10.76
Bjellqvist 10.526
Wikipedia 11.008
Rodwell 10.847
Grimsley 10.818
Solomon 10.906
Lehninger 10.862
Nozaki 10.628
DTASelect 10.511
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.701
Patrickios 10.526
IPC_peptide 10.906
IPC2_peptide 9.531
IPC2.peptide.svr19 8.617
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8600
0
8600
4400537
44
5001
511.7
56.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.009 ± 0.026
0.968 ± 0.008
5.651 ± 0.017
6.294 ± 0.025
3.301 ± 0.015
7.174 ± 0.025
2.359 ± 0.011
5.097 ± 0.018
5.164 ± 0.026
8.655 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.034 ± 0.009
3.87 ± 0.015
6.529 ± 0.03
4.029 ± 0.023
5.731 ± 0.024
9.512 ± 0.035
6.088 ± 0.018
5.601 ± 0.019
1.345 ± 0.008
2.59 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here