Lake Sarah-associated circular virus-6

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQK0|A0A140AQK0_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-6 OX=1685783 PE=3 SV=1
MM1 pKa = 7.54EE2 pKa = 6.32PIDD5 pKa = 4.15EE6 pKa = 4.85ASQPMEE12 pKa = 5.35DD13 pKa = 4.49DD14 pKa = 4.25LGVMSPKK21 pKa = 10.41KK22 pKa = 10.27KK23 pKa = 10.3FRR25 pKa = 11.84ATAKK29 pKa = 10.37NFALTFPQCDD39 pKa = 3.65MNKK42 pKa = 9.73EE43 pKa = 3.89DD44 pKa = 4.6AFQKK48 pKa = 10.54IMDD51 pKa = 3.94EE52 pKa = 4.25HH53 pKa = 7.49KK54 pKa = 10.24PAYY57 pKa = 10.21LCVAEE62 pKa = 5.14EE63 pKa = 4.02EE64 pKa = 4.77HH65 pKa = 7.35KK66 pKa = 11.06DD67 pKa = 3.9GSPHH71 pKa = 5.42LHH73 pKa = 6.31ALISFSSKK81 pKa = 11.04KK82 pKa = 10.21NIKK85 pKa = 9.73DD86 pKa = 3.4PKK88 pKa = 10.78HH89 pKa = 6.48FDD91 pKa = 3.31LSDD94 pKa = 3.62NEE96 pKa = 4.77STFHH100 pKa = 7.44CNIQSCKK107 pKa = 10.38SVADD111 pKa = 3.42WKK113 pKa = 10.99RR114 pKa = 11.84YY115 pKa = 7.68IKK117 pKa = 10.49KK118 pKa = 10.07DD119 pKa = 3.15GKK121 pKa = 10.62YY122 pKa = 9.96KK123 pKa = 9.2EE124 pKa = 4.55TEE126 pKa = 3.81EE127 pKa = 4.98SFVLDD132 pKa = 3.63DD133 pKa = 3.88HH134 pKa = 7.68RR135 pKa = 11.84LGQRR139 pKa = 11.84QKK141 pKa = 11.11LYY143 pKa = 11.38NDD145 pKa = 4.12FCWSKK150 pKa = 9.98TYY152 pKa = 10.28IEE154 pKa = 4.17RR155 pKa = 11.84NEE157 pKa = 4.13RR158 pKa = 11.84RR159 pKa = 11.84DD160 pKa = 3.19IDD162 pKa = 3.65WPVTIRR168 pKa = 11.84DD169 pKa = 4.14SEE171 pKa = 4.39GHH173 pKa = 4.92EE174 pKa = 4.02HH175 pKa = 5.66TMEE178 pKa = 4.56RR179 pKa = 11.84PLPDD183 pKa = 3.36EE184 pKa = 4.49QGLLTKK190 pKa = 10.11KK191 pKa = 9.95RR192 pKa = 11.84NWWIVSPPNAGKK204 pKa = 8.6TYY206 pKa = 9.56WVNKK210 pKa = 6.92QFKK213 pKa = 7.99GQKK216 pKa = 9.48IFMRR220 pKa = 11.84EE221 pKa = 3.63NKK223 pKa = 9.26EE224 pKa = 4.22YY225 pKa = 10.34AWEE228 pKa = 5.02DD229 pKa = 3.67YY230 pKa = 10.95DD231 pKa = 5.08HH232 pKa = 7.34EE233 pKa = 5.61DD234 pKa = 4.58IIIVDD239 pKa = 4.12DD240 pKa = 4.09STMKK244 pKa = 10.18WEE246 pKa = 4.16EE247 pKa = 3.75LSAVCNTYY255 pKa = 10.74TNLKK259 pKa = 8.95QVPGKK264 pKa = 10.01IRR266 pKa = 11.84YY267 pKa = 8.44KK268 pKa = 10.44NLYY271 pKa = 7.96WKK273 pKa = 10.2QNHH276 pKa = 5.82ARR278 pKa = 11.84NIIILSNLKK287 pKa = 10.37PIDD290 pKa = 4.15CYY292 pKa = 11.28GQNTTILNAVLEE304 pKa = 4.35RR305 pKa = 11.84FVVLEE310 pKa = 3.91IDD312 pKa = 3.66NLINNN317 pKa = 4.66

Molecular weight: 37.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQK0|A0A140AQK0_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-6 OX=1685783 PE=3 SV=1
MM1 pKa = 7.47TSDD4 pKa = 3.94LQRR7 pKa = 11.84KK8 pKa = 9.22RR9 pKa = 11.84NLSSFGKK16 pKa = 10.16SFSDD20 pKa = 3.85LFMPNKK26 pKa = 9.63NKK28 pKa = 9.8RR29 pKa = 11.84RR30 pKa = 11.84NVQFGGPAKK39 pKa = 9.87RR40 pKa = 11.84AKK42 pKa = 9.88NSVVAGSFRR51 pKa = 11.84SPTVSNSAGGVIRR64 pKa = 11.84GGSQGRR70 pKa = 11.84WSSLPFQRR78 pKa = 11.84NGYY81 pKa = 8.04KK82 pKa = 10.07QYY84 pKa = 11.08INRR87 pKa = 11.84LLNPPVIITDD97 pKa = 3.2RR98 pKa = 11.84WTGLLKK104 pKa = 9.62QTVTATPDD112 pKa = 2.93LPTPVNKK119 pKa = 9.94IQNFFIDD126 pKa = 3.41SMTIGEE132 pKa = 4.44NVSAVLTNILKK143 pKa = 8.1THH145 pKa = 6.36SGHH148 pKa = 6.2GLGMQGATMTSSTFAQAILTLNTPINTVQQTFTQSFRR185 pKa = 11.84YY186 pKa = 9.28QKK188 pKa = 9.62QNKK191 pKa = 6.51VTFQNMTNNNVKK203 pKa = 8.74VTVFCFAVRR212 pKa = 11.84DD213 pKa = 3.71LRR215 pKa = 11.84TSYY218 pKa = 11.27NILTPYY224 pKa = 7.03TTLDD228 pKa = 3.93LAPGTSPNLPILPAASNAVLEE249 pKa = 4.41QAKK252 pKa = 8.04QTSGNVVQDD261 pKa = 3.82SLCVSTGFDD270 pKa = 3.59GDD272 pKa = 4.1SLNSFVAASTDD283 pKa = 3.2AQTCNWFLKK292 pKa = 10.7NPWSYY297 pKa = 11.0YY298 pKa = 7.22KK299 pKa = 10.16WRR301 pKa = 11.84MEE303 pKa = 3.81YY304 pKa = 10.43RR305 pKa = 11.84KK306 pKa = 9.94VSQVSFMLPAGQGTCTRR323 pKa = 11.84YY324 pKa = 9.68FNHH327 pKa = 6.41SFKK330 pKa = 11.23LNNKK334 pKa = 7.69WSFYY338 pKa = 8.8TEE340 pKa = 4.38WAQHH344 pKa = 5.3PAFTRR349 pKa = 11.84FYY351 pKa = 10.55LIQTEE356 pKa = 4.37TQPMSTVTTGVLPSQQGFLAYY377 pKa = 9.69PPATVGLLVEE387 pKa = 4.74SKK389 pKa = 9.56TVIQRR394 pKa = 11.84VSGVTRR400 pKa = 11.84ASHH403 pKa = 5.97LRR405 pKa = 11.84EE406 pKa = 4.08PLVDD410 pKa = 3.61PTPITGNASTGAADD424 pKa = 5.37FIAPASLVSGALNMASEE441 pKa = 4.48EE442 pKa = 4.17TDD444 pKa = 3.11QKK446 pKa = 10.32QAGVFFF452 pKa = 5.24

Molecular weight: 49.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

769

317

452

384.5

43.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.982 ± 1.0

1.43 ± 0.497

5.202 ± 1.916

4.551 ± 2.332

5.072 ± 0.821

5.202 ± 1.307

2.081 ± 0.887

4.941 ± 1.277

7.022 ± 2.164

7.282 ± 0.42

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.117

7.022 ± 0.052

5.202 ± 0.501

5.332 ± 0.988

4.551 ± 0.086

7.672 ± 1.475

7.932 ± 2.246

5.982 ± 1.201

2.081 ± 0.484

3.121 ± 0.424
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski