Dolichos yellow mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I4HY36|A0A2I4HY36_9GEMI AC4 OS=Dolichos yellow mosaic virus OX=333968 GN=AC4 PE=3 SV=1
MM1 pKa = 7.45GLCISMPSSSSRR13 pKa = 11.84VRR15 pKa = 11.84PSSEE19 pKa = 3.71MQDD22 pKa = 2.94ISTLPILIAHH32 pKa = 7.12RR33 pKa = 11.84FSMEE37 pKa = 3.39MSRR40 pKa = 11.84EE41 pKa = 3.89QRR43 pKa = 11.84AHH45 pKa = 6.65LMSNPTSPRR54 pKa = 11.84TVITSTGEE62 pKa = 3.97HH63 pKa = 6.09FRR65 pKa = 11.84STDD68 pKa = 3.4DD69 pKa = 3.69LLEE72 pKa = 4.17EE73 pKa = 4.48VVRR76 pKa = 11.84QLTMLL81 pKa = 4.15

Molecular weight: 9.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I4HY36|A0A2I4HY36_9GEMI AC4 OS=Dolichos yellow mosaic virus OX=333968 GN=AC4 PE=3 SV=1
MM1 pKa = 6.87YY2 pKa = 10.03RR3 pKa = 11.84SRR5 pKa = 11.84STPLSARR12 pKa = 11.84RR13 pKa = 11.84PLVNPFVRR21 pKa = 11.84EE22 pKa = 3.81RR23 pKa = 11.84QFVPRR28 pKa = 11.84RR29 pKa = 11.84TPRR32 pKa = 11.84FFRR35 pKa = 11.84KK36 pKa = 9.14RR37 pKa = 11.84VHH39 pKa = 5.39ATHH42 pKa = 5.94RR43 pKa = 11.84TSRR46 pKa = 11.84RR47 pKa = 11.84LSYY50 pKa = 10.72EE51 pKa = 3.6RR52 pKa = 11.84VDD54 pKa = 3.53RR55 pKa = 11.84PMSFNVVVEE64 pKa = 4.34RR65 pKa = 11.84QHH67 pKa = 6.41GDD69 pKa = 2.55HH70 pKa = 6.51MALVNNQDD78 pKa = 2.71ITSFIGYY85 pKa = 7.46PIRR88 pKa = 11.84GFNEE92 pKa = 3.81GRR94 pKa = 11.84CRR96 pKa = 11.84DD97 pKa = 4.01YY98 pKa = 11.36IKK100 pKa = 11.05LLTLNVSGMITVRR113 pKa = 11.84SLSTDD118 pKa = 2.68APMSSNGIVNGTFVLSFVLDD138 pKa = 3.96KK139 pKa = 11.09KK140 pKa = 10.64PYY142 pKa = 10.62LPDD145 pKa = 3.6GVNTLPSFAEE155 pKa = 3.89LFGPFSAAYY164 pKa = 9.84FNLRR168 pKa = 11.84LLDD171 pKa = 3.62SQRR174 pKa = 11.84EE175 pKa = 4.13RR176 pKa = 11.84FRR178 pKa = 11.84LLGSVKK184 pKa = 10.36KK185 pKa = 9.91HH186 pKa = 4.96VSCGADD192 pKa = 3.0EE193 pKa = 4.7VEE195 pKa = 4.56VPFKK199 pKa = 10.73FKK201 pKa = 10.04RR202 pKa = 11.84TLSTTRR208 pKa = 11.84STMWATFKK216 pKa = 11.08DD217 pKa = 3.44VDD219 pKa = 3.91MGNSGGNYY227 pKa = 9.9RR228 pKa = 11.84NISKK232 pKa = 10.35NAILVSYY239 pKa = 10.49AFVSMHH245 pKa = 6.68NIKK248 pKa = 10.6CEE250 pKa = 3.87PYY252 pKa = 8.54VQYY255 pKa = 10.63EE256 pKa = 3.86LSYY259 pKa = 11.02FGG261 pKa = 4.73

Molecular weight: 30.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1634

81

365

204.3

23.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.447 ± 0.669

1.836 ± 0.323

5.141 ± 0.379

4.835 ± 0.328

5.141 ± 0.721

4.835 ± 0.399

3.182 ± 0.349

5.08 ± 0.436

5.202 ± 0.533

7.466 ± 0.749

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.754 ± 0.458

5.63 ± 0.428

5.141 ± 0.35

3.733 ± 0.5

8.017 ± 0.983

8.996 ± 0.626

6.12 ± 0.492

6.671 ± 0.509

1.224 ± 0.198

3.55 ± 0.378
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski