Torque teno mini virus 12
Average proteome isoelectric point is 7.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8DMR2|A8DMR2_9VIRU Capsid protein OS=Torque teno mini virus 12 OX=2065038 PE=3 SV=1
MM1 pKa = 7.57 SDD3 pKa = 3.6 YY4 pKa = 10.96 YY5 pKa = 11.19 KK6 pKa = 10.95 KK7 pKa = 9.97 LTIGPRR13 pKa = 11.84 HH14 pKa = 6.06 AQLNWTNAIVTLHH27 pKa = 7.25 DD28 pKa = 4.63 LQCQCDD34 pKa = 4.34 DD35 pKa = 4.56 PLCHH39 pKa = 6.74 TIEE42 pKa = 4.38 TIINQEE48 pKa = 3.73 KK49 pKa = 10.26 SLKK52 pKa = 9.43 FSKK55 pKa = 10.69 GLSEE59 pKa = 4.87 KK60 pKa = 10.32 IKK62 pKa = 10.18 PCLTTGEE69 pKa = 4.25 AGGVDD74 pKa = 3.68 VVEE77 pKa = 5.24 DD78 pKa = 4.18 FGDD81 pKa = 4.33 DD82 pKa = 3.25 EE83 pKa = 5.07 LEE85 pKa = 3.96 RR86 pKa = 11.84 LFAEE90 pKa = 5.37 DD91 pKa = 3.6 GTGDD95 pKa = 3.59 TDD97 pKa = 3.5 TGG99 pKa = 3.62
Molecular weight: 10.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.403
IPC2_protein 4.431
IPC_protein 4.342
Toseland 4.164
ProMoST 4.482
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.228
Rodwell 4.177
Grimsley 4.075
Solomon 4.304
Lehninger 4.266
Nozaki 4.431
DTASelect 4.635
Thurlkill 4.19
EMBOSS 4.24
Sillero 4.469
Patrickios 3.592
IPC_peptide 4.304
IPC2_peptide 4.444
IPC2.peptide.svr19 4.397
Protein with the highest isoelectric point:
>tr|A8DMR4|A8DMR4_9VIRU ORF4 OS=Torque teno mini virus 12 OX=2065038 PE=4 SV=1
MM1 pKa = 7.2 FVIHH5 pKa = 7.14 RR6 pKa = 11.84 PNQTFLNPVTSYY18 pKa = 10.49 RR19 pKa = 11.84 QLYY22 pKa = 10.1 CRR24 pKa = 11.84 IQNIPSNTTCKK35 pKa = 10.27 PLTSEE40 pKa = 4.41 GDD42 pKa = 3.7 SLQKK46 pKa = 10.62 RR47 pKa = 11.84 LQNEE51 pKa = 4.37 SKK53 pKa = 9.4 KK54 pKa = 9.04 TMKK57 pKa = 10.38 LNKK60 pKa = 9.57 LFCHH64 pKa = 6.4 LQDD67 pKa = 3.49 KK68 pKa = 8.85 LQWTSSSKK76 pKa = 10.32 PPRR79 pKa = 11.84 QHH81 pKa = 6.25 RR82 pKa = 11.84 QRR84 pKa = 11.84 THH86 pKa = 5.69 RR87 pKa = 11.84 RR88 pKa = 11.84 RR89 pKa = 11.84 KK90 pKa = 9.68 KK91 pKa = 9.92 SRR93 pKa = 11.84 KK94 pKa = 7.87 HH95 pKa = 6.05 CSSSSSDD102 pKa = 3.15 TSEE105 pKa = 4.58 SNGNSDD111 pKa = 4.45 TEE113 pKa = 4.38 SSSCC117 pKa = 4.47
Molecular weight: 13.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.254
IPC2_protein 9.428
IPC_protein 9.516
Toseland 10.511
ProMoST 10.014
Dawson 10.599
Bjellqvist 10.218
Wikipedia 10.716
Rodwell 11.096
Grimsley 10.613
Solomon 10.643
Lehninger 10.628
Nozaki 10.526
DTASelect 10.189
Thurlkill 10.496
EMBOSS 10.891
Sillero 10.526
Patrickios 10.847
IPC_peptide 10.657
IPC2_peptide 9.209
IPC2.peptide.svr19 8.379
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
941
64
661
235.3
27.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.507 ± 1.032
1.913 ± 0.85
4.676 ± 1.435
5.101 ± 0.756
4.038 ± 0.585
4.145 ± 1.234
2.125 ± 0.803
4.676 ± 0.891
8.077 ± 0.39
9.245 ± 0.442
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.913 ± 0.409
5.526 ± 0.85
7.651 ± 1.558
5.101 ± 0.694
6.908 ± 1.233
6.482 ± 2.787
8.608 ± 0.616
3.401 ± 0.497
2.019 ± 0.409
4.888 ± 1.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here