Babaco mosaic virus
Average proteome isoelectric point is 7.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4ZU36|A0A2H4ZU36_9VIRU Coat protein OS=Babaco mosaic virus OX=2060511 PE=3 SV=1
MM1 pKa = 7.7 ASLGEE6 pKa = 4.09 IKK8 pKa = 10.09 IALAVFVLALAIFTLLNSNKK28 pKa = 9.79 RR29 pKa = 11.84 EE30 pKa = 3.97 TCLVEE35 pKa = 4.52 VSATLSRR42 pKa = 11.84 VSGQDD47 pKa = 3.07 CRR49 pKa = 11.84 HH50 pKa = 5.61 ITPEE54 pKa = 4.35 IIQALGEE61 pKa = 4.13 ALVGLRR67 pKa = 11.84 LL68 pKa = 3.54
Molecular weight: 7.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.297
IPC2_protein 6.148
IPC_protein 6.491
Toseland 6.389
ProMoST 6.722
Dawson 6.898
Bjellqvist 6.532
Wikipedia 6.868
Rodwell 6.868
Grimsley 6.415
Solomon 7.0
Lehninger 7.015
Nozaki 6.912
DTASelect 7.132
Thurlkill 7.146
EMBOSS 7.263
Sillero 7.249
Patrickios 4.596
IPC_peptide 7.015
IPC2_peptide 7.0
IPC2.peptide.svr19 6.933
Protein with the highest isoelectric point:
>tr|A0A2H4ZU43|A0A2H4ZU43_9VIRU TGB1 OS=Babaco mosaic virus OX=2060511 PE=4 SV=1
MM1 pKa = 7.56 SEE3 pKa = 4.67 LPRR6 pKa = 11.84 SLTPPSDD13 pKa = 3.4 NSSAILAVAVGIGLALFTFTLLSYY37 pKa = 10.5 KK38 pKa = 10.67 LPVPGDD44 pKa = 4.08 NIHH47 pKa = 6.37 SLPFGGYY54 pKa = 9.28 YY55 pKa = 9.62 RR56 pKa = 11.84 DD57 pKa = 3.71 GTKK60 pKa = 10.35 SISYY64 pKa = 9.84 NSPRR68 pKa = 11.84 SQASASKK75 pKa = 10.28 SVPALLVLALVAAIYY90 pKa = 10.13 GITWGDD96 pKa = 3.32 KK97 pKa = 8.86 NRR99 pKa = 11.84 ARR101 pKa = 11.84 GVRR104 pKa = 11.84 ACPCHH109 pKa = 6.32 LHH111 pKa = 6.73 SPQQ114 pKa = 3.52
Molecular weight: 12.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.878
IPC2_protein 9.107
IPC_protein 9.209
Toseland 9.385
ProMoST 9.326
Dawson 9.721
Bjellqvist 9.56
Wikipedia 9.955
Rodwell 9.838
Grimsley 9.838
Solomon 9.823
Lehninger 9.78
Nozaki 9.56
DTASelect 9.502
Thurlkill 9.545
EMBOSS 9.823
Sillero 9.706
Patrickios 4.507
IPC_peptide 9.809
IPC2_peptide 8.58
IPC2.peptide.svr19 7.866
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2167
68
1539
433.4
48.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.891 ± 0.899
1.569 ± 0.171
3.922 ± 0.599
6.645 ± 1.24
4.707 ± 0.421
5.584 ± 0.417
3.0 ± 0.734
5.722 ± 0.534
6.876 ± 1.427
9.737 ± 1.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.8 ± 0.362
4.107 ± 0.966
6.184 ± 0.601
4.107 ± 0.553
4.246 ± 0.428
8.491 ± 1.255
6.184 ± 0.49
5.215 ± 0.707
1.061 ± 0.24
2.953 ± 0.592
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here