Streptococcus satellite phage Javan437

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZTE1|A0A4D5ZTE1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan437 OX=2558704 GN=JavanS437_0019 PE=4 SV=1
MM1 pKa = 7.54IADD4 pKa = 3.78KK5 pKa = 11.39LEE7 pKa = 4.85AISTNLEE14 pKa = 4.11EE15 pKa = 4.13IQEE18 pKa = 4.13ILVGNGTALAKK29 pKa = 10.45RR30 pKa = 11.84EE31 pKa = 3.86ISKK34 pKa = 10.38VLNDD38 pKa = 4.47LDD40 pKa = 5.59DD41 pKa = 4.4IYY43 pKa = 11.79NEE45 pKa = 4.13LTSTEE50 pKa = 3.99YY51 pKa = 10.82HH52 pKa = 5.9EE53 pKa = 4.26QQEE56 pKa = 4.54TLKK59 pKa = 10.72EE60 pKa = 3.7QTEE63 pKa = 4.21RR64 pKa = 11.84MKK66 pKa = 10.83QRR68 pKa = 11.84VVSEE72 pKa = 4.31VIGEE76 pKa = 4.27LEE78 pKa = 3.68WRR80 pKa = 11.84EE81 pKa = 3.45YY82 pKa = 11.05SYY84 pKa = 11.97YY85 pKa = 10.94KK86 pKa = 10.66NFWGDD91 pKa = 3.24MDD93 pKa = 5.26LLKK96 pKa = 10.88CLSNFDD102 pKa = 3.56GFLFISTYY110 pKa = 10.01LAEE113 pKa = 4.1ITKK116 pKa = 10.6EE117 pKa = 3.51NDD119 pKa = 2.93YY120 pKa = 11.52PMDD123 pKa = 4.03KK124 pKa = 10.74NQVLNYY130 pKa = 9.47VWEE133 pKa = 4.22LLAIDD138 pKa = 3.6IAKK141 pKa = 10.3KK142 pKa = 10.58KK143 pKa = 9.91RR144 pKa = 11.84DD145 pKa = 3.56KK146 pKa = 11.32KK147 pKa = 10.92NLLGLWTSSIEE158 pKa = 4.07YY159 pKa = 8.46TRR161 pKa = 11.84GMMIYY166 pKa = 9.87EE167 pKa = 4.03EE168 pKa = 4.39HH169 pKa = 6.67

Molecular weight: 19.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZNL6|A0A4D5ZNL6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan437 OX=2558704 GN=JavanS437_0011 PE=4 SV=1
MM1 pKa = 7.87KK2 pKa = 9.27ITEE5 pKa = 4.15YY6 pKa = 10.16TKK8 pKa = 10.79KK9 pKa = 10.58DD10 pKa = 2.93GSTVYY15 pKa = 10.37RR16 pKa = 11.84SSVYY20 pKa = 10.68LGIDD24 pKa = 3.15TVTGKK29 pKa = 10.24KK30 pKa = 10.27VKK32 pKa = 8.07TTISGRR38 pKa = 11.84TKK40 pKa = 10.61RR41 pKa = 11.84EE42 pKa = 3.76LKK44 pKa = 10.7AKK46 pKa = 10.16ALQAQIDD53 pKa = 4.0FEE55 pKa = 5.43KK56 pKa = 11.01DD57 pKa = 2.64GSTVYY62 pKa = 10.61KK63 pKa = 10.4AVEE66 pKa = 3.74IKK68 pKa = 9.8TYY70 pKa = 11.23AEE72 pKa = 4.37LVEE75 pKa = 4.62NWLEE79 pKa = 4.57TYY81 pKa = 10.53CHH83 pKa = 5.45TVKK86 pKa = 10.81KK87 pKa = 9.8ATLTIIKK94 pKa = 10.1SRR96 pKa = 11.84LKK98 pKa = 10.87NYY100 pKa = 9.35LLPAFGDD107 pKa = 3.76YY108 pKa = 10.88KK109 pKa = 10.73LDD111 pKa = 3.6KK112 pKa = 9.52LTPPVIQKK120 pKa = 9.14QVNQWAKK127 pKa = 10.6EE128 pKa = 3.84YY129 pKa = 9.72NQLGKK134 pKa = 10.69GYY136 pKa = 9.89QEE138 pKa = 3.99YY139 pKa = 9.14PQLNSLNKK147 pKa = 10.15RR148 pKa = 11.84ILKK151 pKa = 9.76YY152 pKa = 10.38AVSLQVIPFNPARR165 pKa = 11.84DD166 pKa = 3.53IIVPRR171 pKa = 11.84RR172 pKa = 11.84KK173 pKa = 10.05AKK175 pKa = 10.24EE176 pKa = 4.02GQKK179 pKa = 10.55LKK181 pKa = 11.17YY182 pKa = 10.43LDD184 pKa = 4.8DD185 pKa = 5.74DD186 pKa = 4.04NLKK189 pKa = 10.78KK190 pKa = 10.49FLNYY194 pKa = 10.06LDD196 pKa = 5.07RR197 pKa = 11.84LPNTYY202 pKa = 10.78KK203 pKa = 10.76NFYY206 pKa = 9.48DD207 pKa = 3.51TVLYY211 pKa = 8.15KK212 pKa = 10.45TLLATGLRR220 pKa = 11.84IRR222 pKa = 11.84EE223 pKa = 4.08CLALKK228 pKa = 10.05WSDD231 pKa = 3.08IDD233 pKa = 4.02LQNGTLDD240 pKa = 3.42VNKK243 pKa = 9.41TLNCEE248 pKa = 4.24KK249 pKa = 10.58EE250 pKa = 4.16VTTPKK255 pKa = 9.67TKK257 pKa = 10.55SSVRR261 pKa = 11.84MIDD264 pKa = 3.65LDD266 pKa = 3.84NKK268 pKa = 8.1TVLMLRR274 pKa = 11.84LYY276 pKa = 10.7KK277 pKa = 10.34NRR279 pKa = 11.84QAQVGRR285 pKa = 11.84EE286 pKa = 3.43IGLTYY291 pKa = 10.66KK292 pKa = 10.49KK293 pKa = 10.61VFSNSFDD300 pKa = 3.44EE301 pKa = 4.33YY302 pKa = 10.78RR303 pKa = 11.84DD304 pKa = 3.44ARR306 pKa = 11.84ALRR309 pKa = 11.84SRR311 pKa = 11.84LEE313 pKa = 3.75KK314 pKa = 10.29HH315 pKa = 6.29LKK317 pKa = 10.4LSEE320 pKa = 4.09CPRR323 pKa = 11.84LTFHH327 pKa = 7.91AFRR330 pKa = 11.84HH331 pKa = 4.54THH333 pKa = 6.71ASILLNAGLPYY344 pKa = 10.43KK345 pKa = 10.2EE346 pKa = 3.68IQTRR350 pKa = 11.84LGHH353 pKa = 5.05SQISITMDD361 pKa = 3.56TYY363 pKa = 11.78SHH365 pKa = 7.28LSKK368 pKa = 10.85DD369 pKa = 3.51NKK371 pKa = 10.49KK372 pKa = 10.17NATSFYY378 pKa = 9.85EE379 pKa = 3.99KK380 pKa = 10.33AIEE383 pKa = 4.04KK384 pKa = 10.35LKK386 pKa = 11.16SSS388 pKa = 3.82

Molecular weight: 44.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

3008

41

507

143.2

16.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.652 ± 0.407

0.465 ± 0.127

5.751 ± 0.36

9.176 ± 0.748

3.956 ± 0.438

4.82 ± 0.35

1.563 ± 0.294

7.148 ± 0.492

9.608 ± 0.622

10.173 ± 0.708

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.386

4.754 ± 0.34

2.726 ± 0.434

4.555 ± 0.331

5.386 ± 0.295

5.618 ± 0.212

5.918 ± 0.416

4.787 ± 0.418

0.931 ± 0.173

4.588 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski