Bradymonadales bacterium V1718

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Bradymonadales; unclassified Bradymonadales

Average proteome isoelectric point is 5.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4727 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8XNV3|A0A5B8XNV3_9DELT Uncharacterized protein OS=Bradymonadales bacterium V1718 OX=2600177 GN=FRD01_08150 PE=4 SV=1
MM1 pKa = 7.29FRR3 pKa = 11.84LLILVALFSLGSGCSGCDD21 pKa = 3.62DD22 pKa = 4.61DD23 pKa = 6.62SGGIPNSDD31 pKa = 3.68AGNNTQDD38 pKa = 3.78MADD41 pKa = 3.85DD42 pKa = 4.61ANNLGDD48 pKa = 4.6GGQSDD53 pKa = 3.83MGEE56 pKa = 4.17PDD58 pKa = 3.91GGPSDD63 pKa = 3.9MGEE66 pKa = 4.15PDD68 pKa = 4.9ANTCSTVEE76 pKa = 4.27CNGVCCGEE84 pKa = 4.11GQEE87 pKa = 4.99CFQDD91 pKa = 4.2LCLDD95 pKa = 4.06PCAGTRR101 pKa = 11.84CGAGFEE107 pKa = 4.51LCCAEE112 pKa = 5.31ADD114 pKa = 3.37ICLGQACLTPGADD127 pKa = 3.27CTVTEE132 pKa = 4.3EE133 pKa = 4.42CAVEE137 pKa = 4.69EE138 pKa = 4.1ICEE141 pKa = 4.17PTVGKK146 pKa = 9.94CVPRR150 pKa = 11.84DD151 pKa = 3.37AVEE154 pKa = 3.94VCEE157 pKa = 5.07FIPPIGPFEE166 pKa = 4.34PEE168 pKa = 4.43VGCFWPNPAPTVNPDD183 pKa = 3.13SVHH186 pKa = 5.96VVVAPIVGNLTDD198 pKa = 4.83DD199 pKa = 4.19NGDD202 pKa = 3.61GLTNTDD208 pKa = 4.33DD209 pKa = 3.51VPEE212 pKa = 4.2IVFLTRR218 pKa = 11.84TSGCCDD224 pKa = 2.54KK225 pKa = 11.05RR226 pKa = 11.84GTLRR230 pKa = 11.84IVDD233 pKa = 4.14GRR235 pKa = 11.84CGANGEE241 pKa = 4.3MTTIASLDD249 pKa = 3.94SVVMTNDD256 pKa = 3.23AAPALGDD263 pKa = 3.82LDD265 pKa = 4.4GDD267 pKa = 4.1GVPEE271 pKa = 4.04IVAVKK276 pKa = 10.01GQNLQTNQKK285 pKa = 8.15VTPQGLVAWKK295 pKa = 10.2RR296 pKa = 11.84VTPDD300 pKa = 2.87GATWEE305 pKa = 4.28PMWEE309 pKa = 3.9NDD311 pKa = 3.24EE312 pKa = 4.36YY313 pKa = 10.6PTFGVHH319 pKa = 5.27TRR321 pKa = 11.84GGPTVGIADD330 pKa = 4.75LDD332 pKa = 4.05GDD334 pKa = 4.25GNPEE338 pKa = 3.87VFVGNVVLNGQDD350 pKa = 5.37GNLKK354 pKa = 9.34WDD356 pKa = 3.7GVANSQTPVGIGNNAFLGPSSIAADD381 pKa = 3.33VDD383 pKa = 4.11LDD385 pKa = 3.84GMQEE389 pKa = 3.91VMAGNTLYY397 pKa = 11.1SHH399 pKa = 7.7DD400 pKa = 3.59GTVRR404 pKa = 11.84WTYY407 pKa = 10.95EE408 pKa = 3.83YY409 pKa = 7.39TTSNSTCQGGLPCDD423 pKa = 4.25GYY425 pKa = 9.34TAVAEE430 pKa = 4.09FDD432 pKa = 3.8GDD434 pKa = 3.95PEE436 pKa = 4.47GEE438 pKa = 3.93IVIIRR443 pKa = 11.84LGEE446 pKa = 4.23VFVLNHH452 pKa = 7.19DD453 pKa = 3.95GTLFWQQQIVKK464 pKa = 9.92DD465 pKa = 3.55DD466 pKa = 3.64CTRR469 pKa = 11.84NEE471 pKa = 4.39SGPPTIADD479 pKa = 3.35FDD481 pKa = 4.68GDD483 pKa = 3.82GRR485 pKa = 11.84PEE487 pKa = 4.1IGTAAADD494 pKa = 4.85FYY496 pKa = 11.32TVLDD500 pKa = 4.83LDD502 pKa = 4.91CDD504 pKa = 3.74TDD506 pKa = 3.12TWEE509 pKa = 5.97DD510 pKa = 3.53DD511 pKa = 3.56GCFARR516 pKa = 11.84GVLWATPNQDD526 pKa = 2.77CSSRR530 pKa = 11.84VTASSVFDD538 pKa = 3.8FEE540 pKa = 5.69GDD542 pKa = 3.31GKK544 pKa = 11.16AEE546 pKa = 3.76MVYY549 pKa = 10.93ADD551 pKa = 3.99EE552 pKa = 4.25QNFRR556 pKa = 11.84IFDD559 pKa = 3.9GTTGAILFDD568 pKa = 4.82DD569 pKa = 5.31PSHH572 pKa = 6.98SSNTRR577 pKa = 11.84IEE579 pKa = 4.18MPIVADD585 pKa = 3.51VDD587 pKa = 3.47NDD589 pKa = 3.89GNSEE593 pKa = 4.07IVVPSATAQSIKK605 pKa = 10.46VFKK608 pKa = 10.57DD609 pKa = 3.49PSDD612 pKa = 2.9NWVRR616 pKa = 11.84TRR618 pKa = 11.84RR619 pKa = 11.84IWNQHH624 pKa = 4.85AYY626 pKa = 10.15AVTNINEE633 pKa = 4.82DD634 pKa = 3.56GTVPAQPDD642 pKa = 3.88INWLNGRR649 pKa = 11.84LNNFRR654 pKa = 11.84QNIQPGGIFDD664 pKa = 4.5APNLLIEE671 pKa = 4.51SVDD674 pKa = 3.65VRR676 pKa = 11.84GLGCGEE682 pKa = 4.16DD683 pKa = 3.53SEE685 pKa = 5.3VIIRR689 pKa = 11.84VTVSNAGALGVAPGNTLIRR708 pKa = 11.84IYY710 pKa = 10.58GSSGGDD716 pKa = 3.16TIVIDD721 pKa = 4.09DD722 pKa = 3.94TTLTTRR728 pKa = 11.84LLPGQRR734 pKa = 11.84EE735 pKa = 4.42VVEE738 pKa = 3.98VTYY741 pKa = 10.6AVPADD746 pKa = 3.28WVTNGFEE753 pKa = 3.75IGAIIDD759 pKa = 3.57PDD761 pKa = 3.5ATINEE766 pKa = 4.48CNEE769 pKa = 4.32DD770 pKa = 3.99DD771 pKa = 4.03NQGSFDD777 pKa = 3.55GANVVFSAPEE787 pKa = 4.01LEE789 pKa = 4.58VTTLTATGTTCGLTLQMPIAFTVRR813 pKa = 11.84NSGTDD818 pKa = 3.28TVPANVPLVVTGTFQGTTVEE838 pKa = 4.23IARR841 pKa = 11.84VTTSAEE847 pKa = 3.87LLVGEE852 pKa = 4.86EE853 pKa = 4.17EE854 pKa = 4.79DD855 pKa = 5.22FNLVWTVDD863 pKa = 3.7PGAPAQDD870 pKa = 3.34VTITVTVDD878 pKa = 3.14PDD880 pKa = 3.71KK881 pKa = 11.15EE882 pKa = 4.67VYY884 pKa = 10.52DD885 pKa = 4.36CDD887 pKa = 3.67EE888 pKa = 4.18QEE890 pKa = 4.21ALSVVEE896 pKa = 4.1NCRR899 pKa = 11.84ISGG902 pKa = 3.5

Molecular weight: 95.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8XS27|A0A5B8XS27_9DELT MBL fold metallo-hydrolase OS=Bradymonadales bacterium V1718 OX=2600177 GN=FRD01_10675 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.95RR4 pKa = 11.84TYY6 pKa = 10.24QPSKK10 pKa = 7.88VKK12 pKa = 10.25RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84THH17 pKa = 5.65GFRR20 pKa = 11.84ARR22 pKa = 11.84MADD25 pKa = 2.99AGGRR29 pKa = 11.84KK30 pKa = 8.93VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 7.87RR42 pKa = 11.84LAVQRR47 pKa = 11.84HH48 pKa = 4.75KK49 pKa = 10.93KK50 pKa = 9.57

Molecular weight: 5.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4727

0

4727

1827870

24

5142

386.7

42.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.412 ± 0.032

1.347 ± 0.039

6.196 ± 0.04

7.604 ± 0.039

4.072 ± 0.02

7.843 ± 0.04

1.989 ± 0.019

4.968 ± 0.023

3.912 ± 0.035

9.821 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.22 ± 0.017

3.263 ± 0.026

4.946 ± 0.026

3.41 ± 0.018

5.955 ± 0.037

6.344 ± 0.027

5.37 ± 0.03

7.537 ± 0.029

1.405 ± 0.017

2.386 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski