Proteome-pI 2.0 - Proteome Isoelectric Point Database

Agrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis (strain S4))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium; Agrobacterium vitis

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 5353 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>sp|B9JR74|UREG_AGRVS Urease accessory protein UreG OS=Agrobacterium vitis (strain S4 / ATCC BAA-846) OX=311402 GN=ureG PE=3 SV=1

MM1 pKa = 7.43IHH3 pKa = 5.38VQKK6 pKa = 10.7DD7 pKa = 3.23DD8 pKa = 3.44SLANVIVNAFANAAAPAAVALDD30 pKa = 4.08PYY32 pKa = 9.74VTGISPEE39 pKa = 4.46SITITANGFGPIIIDD54 pKa = 4.01DD55 pKa = 4.23PQDD58 pKa = 3.54NIMYY62 pKa = 9.68IVSTNEE68 pKa = 2.94VDD70 pKa = 5.39LEE72 pKa = 4.3ITTHH76 pKa = 6.39SIVGG80 pKa = 3.55

Molecular weight: 8.39 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|B9K060|B9K060_AGRVS Sulfate permease OS=Agrobacterium vitis (strain S4 / ATCC BAA-846) OX=311402 GN=Avi_5067 PE=4 SV=1

MM1 pKa = 7.23SVARR5 pKa = 11.84RR6 pKa = 11.84DD7 pKa = 4.07LLDD10 pKa = 3.64KK11 pKa = 10.97SATASGHH18 pKa = 5.76EE19 pKa = 3.99NLNLSGRR26 pKa = 11.84PCRR29 pKa = 11.84RR30 pKa = 11.84PVCLYY35 pKa = 10.72RR36 pKa = 11.84PGSSTNDD43 pKa = 3.22PSGEE47 pKa = 3.97IGKK50 pKa = 10.0AEE52 pKa = 4.08YY53 pKa = 9.94FKK55 pKa = 11.03SLPSKK60 pKa = 10.47LVRR63 pKa = 11.84FDD65 pKa = 3.49GALNASQPHH74 pKa = 5.78FLFGNLRR81 pKa = 11.84EE82 pKa = 4.36RR83 pKa = 11.84NTGLKK88 pKa = 10.2LAVPDD93 pKa = 4.33HH94 pKa = 6.32PHH96 pKa = 5.77HH97 pKa = 6.46HH98 pKa = 6.72RR99 pKa = 11.84CLVARR104 pKa = 11.84RR105 pKa = 11.84GHH107 pKa = 4.05THH109 pKa = 5.61GRR111 pKa = 11.84IGLLGLFGEE120 pKa = 4.78VSRR123 pKa = 11.84IIGLLLFHH131 pKa = 6.89SLPFRR136 pKa = 11.84LAALGDD142 pKa = 3.8PVRR145 pKa = 11.84ARR147 pKa = 11.84LTSSDD152 pKa = 3.14

Molecular weight: 16.69 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
5353	0	5353	1783039	38	3761	333.1	36.28

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
11.477 ± 0.044	0.833 ± 0.009	5.791 ± 0.029	5.501 ± 0.028	3.876 ± 0.023	8.186 ± 0.035	2.074 ± 0.016	5.691 ± 0.026	3.851 ± 0.028	10.073 ± 0.041

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.6 ± 0.019	2.929 ± 0.02	4.891 ± 0.025	3.455 ± 0.02	6.447 ± 0.032	6.07 ± 0.032	5.517 ± 0.03	7.151 ± 0.025	1.262 ± 0.015	2.325 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski