Halothiobacillus sp. LS2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Halothiobacillaceae; Halothiobacillus; unclassified Halothiobacillus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2521 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A191ZE22|A0A191ZE22_9GAMM Purine nucleoside permease OS=Halothiobacillus sp. LS2 OX=1860122 GN=A9404_00890 PE=4 SV=1
MM1 pKa = 7.48ANKK4 pKa = 9.82VIQTTPNLGLNIKK17 pKa = 8.21LQKK20 pKa = 9.76VAAAPVEE27 pKa = 4.19LVPALVLDD35 pKa = 3.67INGNVLASLGPGDD48 pKa = 5.32EE49 pKa = 4.55YY50 pKa = 11.06TCQGAPSDD58 pKa = 3.7LTGIKK63 pKa = 10.35LMNFPDD69 pKa = 3.12KK70 pKa = 10.06WGQYY74 pKa = 6.34PTPTFVNNSSFGSSYY89 pKa = 10.38TVYY92 pKa = 10.68LLPIGTFLSTDD103 pKa = 3.45ISWSSVWQGVDD114 pKa = 3.12VSQWCSVAMNVQTPGLLVLDD134 pKa = 5.37VYY136 pKa = 10.62LCCNNGGNPDD146 pKa = 3.33ATATLTLTATTSDD159 pKa = 3.53GATASIDD166 pKa = 3.27IDD168 pKa = 3.77FLVQGFF174 pKa = 3.69

Molecular weight: 18.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A191ZI76|A0A191ZI76_9GAMM Glutathione peroxidase OS=Halothiobacillus sp. LS2 OX=1860122 GN=A9404_09365 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPSIIHH11 pKa = 5.83RR12 pKa = 11.84QRR14 pKa = 11.84THH16 pKa = 5.58GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 9.02TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2521

0

2521

841465

41

3892

333.8

36.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.171 ± 0.062

0.803 ± 0.016

5.808 ± 0.036

5.348 ± 0.048

3.608 ± 0.035

7.835 ± 0.046

2.444 ± 0.024

5.506 ± 0.039

3.146 ± 0.041

10.707 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.023

2.993 ± 0.033

5.289 ± 0.037

4.196 ± 0.04

6.691 ± 0.047

5.437 ± 0.041

5.566 ± 0.041

7.14 ± 0.043

1.41 ± 0.02

2.417 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski