Camel associated porprismacovirus 2
Average proteome isoelectric point is 7.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A1ENU8|A0A0A1ENU8_9VIRU Putative replicase protein OS=Camel associated porprismacovirus 2 OX=2170106 GN=rep PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.43 FKK4 pKa = 10.43 FQSFFDD10 pKa = 3.98 VATSASSMQVIQWSVGGQEE29 pKa = 3.79 VLEE32 pKa = 4.3 RR33 pKa = 11.84 CRR35 pKa = 11.84 HH36 pKa = 4.92 LFGAYY41 pKa = 9.38 KK42 pKa = 10.11 YY43 pKa = 10.17 FKK45 pKa = 10.22 LGKK48 pKa = 9.43 ISVKK52 pKa = 10.1 FVPASTLPVDD62 pKa = 4.11 PQGLSYY68 pKa = 11.14 DD69 pKa = 3.73 ADD71 pKa = 4.07 DD72 pKa = 4.89 PQTVDD77 pKa = 4.88 PRR79 pKa = 11.84 DD80 pKa = 3.3 QMNPGLVRR88 pKa = 11.84 ITNGEE93 pKa = 4.33 DD94 pKa = 2.66 IFTNVSAISAANQEE108 pKa = 4.26 KK109 pKa = 10.73 VYY111 pKa = 10.82 NAMMLDD117 pKa = 3.81 PRR119 pKa = 11.84 WSKK122 pKa = 11.16 FMLQSGFRR130 pKa = 11.84 RR131 pKa = 11.84 EE132 pKa = 4.19 AYY134 pKa = 9.1 PLYY137 pKa = 9.39 WQIGQLSQDD146 pKa = 3.69 YY147 pKa = 9.21 WPGATVNTPVVGEE160 pKa = 4.23 AQATATRR167 pKa = 11.84 PLYY170 pKa = 10.05 IRR172 pKa = 11.84 NVNGAFDD179 pKa = 3.73 TAYY182 pKa = 8.31 GTSGDD187 pKa = 3.53 SSARR191 pKa = 11.84 GIFQTGHH198 pKa = 6.08 RR199 pKa = 11.84 GKK201 pKa = 10.8 LGFLPTDD208 pKa = 4.13 YY209 pKa = 11.31 YY210 pKa = 10.07 QTTVNTRR217 pKa = 11.84 GTASANYY224 pKa = 8.62 GANSVPTVSVITCVLPKK241 pKa = 10.18 AYY243 pKa = 8.45 KK244 pKa = 7.7 TVYY247 pKa = 8.71 YY248 pKa = 10.19 YY249 pKa = 10.71 RR250 pKa = 11.84 VFVTEE255 pKa = 3.83 EE256 pKa = 4.0 VIFAGLRR263 pKa = 11.84 SSPMAEE269 pKa = 3.85 ANGSQTYY276 pKa = 10.79 GLDD279 pKa = 3.04 QFLFANMINIFPGTASTRR297 pKa = 11.84 PSSIEE302 pKa = 3.62 PNKK305 pKa = 10.53 QNDD308 pKa = 4.17 GANLPP313 pKa = 4.06
Molecular weight: 34.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.395
IPC2_protein 7.644
IPC_protein 7.819
Toseland 7.717
ProMoST 8.214
Dawson 8.346
Bjellqvist 8.375
Wikipedia 8.361
Rodwell 8.331
Grimsley 7.585
Solomon 8.565
Lehninger 8.536
Nozaki 8.346
DTASelect 8.302
Thurlkill 8.331
EMBOSS 8.536
Sillero 8.565
Patrickios 3.923
IPC_peptide 8.551
IPC2_peptide 7.38
IPC2.peptide.svr19 7.662
Protein with the highest isoelectric point:
>tr|A0A0A1ENU8|A0A0A1ENU8_9VIRU Putative replicase protein OS=Camel associated porprismacovirus 2 OX=2170106 GN=rep PE=4 SV=1
MM1 pKa = 7.71 GIYY4 pKa = 9.28 MVTAPRR10 pKa = 11.84 KK11 pKa = 8.81 GSKK14 pKa = 10.23 LGTGKK19 pKa = 10.49 EE20 pKa = 4.02 FMKK23 pKa = 10.15 WRR25 pKa = 11.84 TFFYY29 pKa = 10.62 INDD32 pKa = 3.41 VHH34 pKa = 6.96 KK35 pKa = 8.42 WTLGAEE41 pKa = 4.24 TGKK44 pKa = 10.75 GGYY47 pKa = 8.53 KK48 pKa = 9.55 HH49 pKa = 5.4 WQVRR53 pKa = 11.84 LQISGSDD60 pKa = 3.08 NEE62 pKa = 4.51 VFNKK66 pKa = 10.16 IKK68 pKa = 10.32 DD69 pKa = 3.7 EE70 pKa = 4.21 NMFPTAHH77 pKa = 6.65 IEE79 pKa = 4.16 KK80 pKa = 10.76 GSDD83 pKa = 2.47 TWSYY87 pKa = 8.8 EE88 pKa = 4.51 CKK90 pKa = 10.57 EE91 pKa = 4.02 KK92 pKa = 9.96 MHH94 pKa = 6.16 WTSNDD99 pKa = 2.96 TPNILRR105 pKa = 11.84 VRR107 pKa = 11.84 FGHH110 pKa = 5.28 MRR112 pKa = 11.84 WYY114 pKa = 10.05 QKK116 pKa = 11.09 DD117 pKa = 3.37 VIEE120 pKa = 4.48 AVRR123 pKa = 11.84 RR124 pKa = 11.84 QNDD127 pKa = 3.2 RR128 pKa = 11.84 QIAYY132 pKa = 8.88 WYY134 pKa = 10.07 DD135 pKa = 3.19 PEE137 pKa = 4.47 GNKK140 pKa = 10.04 GKK142 pKa = 10.15 SWLVNHH148 pKa = 6.98 LFEE151 pKa = 6.01 SCQAWYY157 pKa = 10.48 VPPTLKK163 pKa = 10.54 SVEE166 pKa = 5.0 GIIQWVASVYY176 pKa = 10.55 INNNYY181 pKa = 10.09 RR182 pKa = 11.84 EE183 pKa = 4.25 ILVIDD188 pKa = 4.65 IPRR191 pKa = 11.84 SWKK194 pKa = 9.63 WSTEE198 pKa = 3.66 LYY200 pKa = 9.73 TAIEE204 pKa = 4.35 TIKK207 pKa = 10.95 DD208 pKa = 3.31 GLVYY212 pKa = 10.41 DD213 pKa = 4.43 PRR215 pKa = 11.84 YY216 pKa = 7.95 HH217 pKa = 6.9 AQMINIRR224 pKa = 11.84 GVKK227 pKa = 9.71 ILVLCNHH234 pKa = 6.06 EE235 pKa = 4.6 PKK237 pKa = 10.53 LDD239 pKa = 3.83 ALSADD244 pKa = 2.93 RR245 pKa = 11.84 WYY247 pKa = 10.94 AVAPALTT254 pKa = 3.74
Molecular weight: 29.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.431
IPC2_protein 8.404
IPC_protein 8.346
Toseland 8.829
ProMoST 8.77
Dawson 9.18
Bjellqvist 9.048
Wikipedia 9.355
Rodwell 9.311
Grimsley 9.209
Solomon 9.282
Lehninger 9.238
Nozaki 9.077
DTASelect 8.96
Thurlkill 9.048
EMBOSS 9.311
Sillero 9.224
Patrickios 4.418
IPC_peptide 9.268
IPC2_peptide 7.732
IPC2.peptide.svr19 7.651
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
567
254
313
283.5
32.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.231 ± 1.105
0.882 ± 0.192
4.938 ± 0.116
4.409 ± 0.961
4.409 ± 1.062
7.231 ± 0.599
1.764 ± 0.89
5.644 ± 1.18
5.644 ± 1.433
5.82 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.646 ± 0.071
5.467 ± 0.029
5.115 ± 0.757
4.586 ± 0.923
5.115 ± 0.255
6.702 ± 1.018
6.702 ± 0.765
7.231 ± 0.346
2.998 ± 1.362
5.467 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here