Actinophytocola xanthii
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7316 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q8CBU3|A0A1Q8CBU3_9PSEU HTH tetR-type domain-containing protein OS=Actinophytocola xanthii OX=1912961 GN=BU204_30105 PE=4 SV=1
MM1 pKa = 6.74 TAHH4 pKa = 7.31 AAVLCVVSAGVTQTGTTVVGSPGNDD29 pKa = 3.3 TIDD32 pKa = 4.52 CGGTDD37 pKa = 3.68 PGKK40 pKa = 9.35 TITGNGGNDD49 pKa = 4.1 TITGSAFNDD58 pKa = 4.36 TIDD61 pKa = 4.5 GGDD64 pKa = 3.78 GNDD67 pKa = 4.09 TITGGTGADD76 pKa = 3.72 SLTGGLGADD85 pKa = 4.5 TISGSAGNDD94 pKa = 3.25 VLIGSSNDD102 pKa = 3.34 GAQDD106 pKa = 3.61 SLDD109 pKa = 3.93 GGDD112 pKa = 6.07 GIDD115 pKa = 3.71 TCQGPAPDD123 pKa = 4.53 PDD125 pKa = 3.03 IHH127 pKa = 7.01 ASCEE131 pKa = 4.18 NTSTPPGTGPGSGLGNATALCTATGGVLSLTVNPVGYY168 pKa = 9.72 ICVFNPLRR176 pKa = 11.84 PADD179 pKa = 3.81 RR180 pKa = 11.84 RR181 pKa = 11.84 VAEE184 pKa = 4.26 ARR186 pKa = 11.84 RR187 pKa = 11.84 ICTGSGGTFVSLLLPVSYY205 pKa = 11.28 ACLLPNARR213 pKa = 11.84 SASS216 pKa = 3.61
Molecular weight: 20.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.783
IPC_protein 3.795
Toseland 3.554
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.821
Rodwell 3.617
Grimsley 3.465
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.279
Thurlkill 3.63
EMBOSS 3.821
Sillero 3.935
Patrickios 0.693
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|A0A1Q8CVM6|A0A1Q8CVM6_9PSEU Uncharacterized protein OS=Actinophytocola xanthii OX=1912961 GN=BU204_07435 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 ARR15 pKa = 11.84 THH17 pKa = 5.69 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 9.97 GRR40 pKa = 11.84 KK41 pKa = 8.97 RR42 pKa = 11.84 LSAA45 pKa = 3.96
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.705
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.427
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.265
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7316
0
7316
2454192
32
6449
335.5
35.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.47 ± 0.045
0.765 ± 0.008
6.047 ± 0.024
5.759 ± 0.031
2.757 ± 0.016
9.282 ± 0.031
2.2 ± 0.012
2.936 ± 0.023
1.454 ± 0.018
10.601 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.651 ± 0.012
1.863 ± 0.018
6.063 ± 0.026
2.596 ± 0.015
8.449 ± 0.036
4.981 ± 0.023
6.191 ± 0.033
9.443 ± 0.032
1.544 ± 0.012
1.947 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here