Rachicladosporium antarcticum
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18598 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V8SNQ6|A0A1V8SNQ6_9PEZI PX domain-containing protein OS=Rachicladosporium antarcticum OX=1507870 GN=B0A48_13872 PE=4 SV=1
MM1 pKa = 7.81 ASPSTILTALLAATTTTSPLPAQTFGPQTVNLVSRR36 pKa = 11.84 LCADD40 pKa = 3.22 QSTSCSDD47 pKa = 3.24 TTYY50 pKa = 10.86 LAWLNTPFSLNITTSQLSLSSAQNVDD76 pKa = 2.8 IDD78 pKa = 4.3 LVNCEE83 pKa = 4.09 VFFTGGDD90 pKa = 3.36 AGPLLTADD98 pKa = 3.89 TPVTLSSGLVDD109 pKa = 3.99 IGNVTCSVSDD119 pKa = 3.38 EE120 pKa = 4.79 GKK122 pKa = 10.56 GQDD125 pKa = 2.92 QAGQIRR131 pKa = 11.84 PGDD134 pKa = 3.62 NATTTWVHH142 pKa = 6.14 GGDD145 pKa = 4.89 AIPVTTTTATGAVPTITGWGGAVVDD170 pKa = 4.92 GGNEE174 pKa = 3.86 TTATGSTPPSTTTYY188 pKa = 9.81 TGGASGLEE196 pKa = 4.28 VARR199 pKa = 11.84 LLAAIAAVVGGWVSLLL215 pKa = 3.61
Molecular weight: 21.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.681
IPC_protein 3.656
Toseland 3.439
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.656
Rodwell 3.49
Grimsley 3.35
Solomon 3.656
Lehninger 3.605
Nozaki 3.808
DTASelect 4.075
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.783
Patrickios 0.769
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.735
Protein with the highest isoelectric point:
>tr|A0A1V8T4W8|A0A1V8T4W8_9PEZI 60S acidic ribosomal protein P2 OS=Rachicladosporium antarcticum OX=1507870 GN=B0A48_08948 PE=3 SV=1
MM1 pKa = 7.29 NGDD4 pKa = 3.64 SGKK7 pKa = 10.74 LPLIDD12 pKa = 4.36 IGDD15 pKa = 3.82 EE16 pKa = 3.88 ARR18 pKa = 11.84 VCRR21 pKa = 11.84 RR22 pKa = 11.84 ANAARR27 pKa = 11.84 PTDD30 pKa = 3.6 NPARR34 pKa = 11.84 TLLKK38 pKa = 9.92 EE39 pKa = 3.96 AYY41 pKa = 8.96 KK42 pKa = 11.05 AEE44 pKa = 4.01 LAEE47 pKa = 4.52 LGIDD51 pKa = 3.58 PGQDD55 pKa = 2.3 RR56 pKa = 11.84 VIFDD60 pKa = 4.34 CLWNIAHH67 pKa = 6.25 VVTEE71 pKa = 4.34 KK72 pKa = 10.08 WRR74 pKa = 11.84 STGAVDD80 pKa = 4.35 FLEE83 pKa = 5.05 ALAQEE88 pKa = 4.49 FDD90 pKa = 3.17 KK91 pKa = 11.4 HH92 pKa = 6.41 GITIVEE98 pKa = 4.29 HH99 pKa = 7.45 DD100 pKa = 4.6 DD101 pKa = 4.01 DD102 pKa = 4.2 GTEE105 pKa = 4.0 ITHH108 pKa = 6.5 EE109 pKa = 4.31 APVQQVVSSRR119 pKa = 11.84 KK120 pKa = 9.25 QIVDD124 pKa = 3.05 KK125 pKa = 11.05 RR126 pKa = 11.84 RR127 pKa = 11.84 VSFDD131 pKa = 3.34 DD132 pKa = 3.89 ARR134 pKa = 11.84 MHH136 pKa = 5.51 EE137 pKa = 4.01 TWIRR141 pKa = 11.84 EE142 pKa = 3.95 RR143 pKa = 11.84 TEE145 pKa = 3.81 TLTQQEE151 pKa = 4.37 DD152 pKa = 3.82 APARR156 pKa = 11.84 LLSQPPVRR164 pKa = 11.84 ASLQRR169 pKa = 11.84 RR170 pKa = 11.84 ARR172 pKa = 11.84 SVSSEE177 pKa = 3.88 HH178 pKa = 7.14 GYY180 pKa = 8.4 ATYY183 pKa = 10.55 QPGRR187 pKa = 11.84 GASLYY192 pKa = 10.45 GRR194 pKa = 11.84 PQSSTDD200 pKa = 3.39 QASDD204 pKa = 3.46 PDD206 pKa = 4.23 GPGDD210 pKa = 3.49 SSMLRR215 pKa = 11.84 RR216 pKa = 11.84 LEE218 pKa = 3.86 VSFEE222 pKa = 4.34 KK223 pKa = 10.53 IATLQEE229 pKa = 3.83 LRR231 pKa = 11.84 EE232 pKa = 4.4 TCEE235 pKa = 3.6 HH236 pKa = 5.44 TRR238 pKa = 11.84 EE239 pKa = 3.88 LRR241 pKa = 11.84 LVNTYY246 pKa = 9.14 FNRR249 pKa = 11.84 WFGNTHH255 pKa = 6.44 LHH257 pKa = 4.7 QQHH260 pKa = 5.89 VARR263 pKa = 11.84 QHH265 pKa = 6.22 SEE267 pKa = 3.97 AEE269 pKa = 4.03 EE270 pKa = 3.69 QHH272 pKa = 6.98 AKK274 pKa = 10.24 VLCQAVFDD282 pKa = 3.94 SWRR285 pKa = 11.84 EE286 pKa = 3.82 AFHH289 pKa = 6.69 EE290 pKa = 4.07 KK291 pKa = 10.33 QILRR295 pKa = 11.84 RR296 pKa = 11.84 EE297 pKa = 4.0 ALEE300 pKa = 4.17 AEE302 pKa = 4.45 EE303 pKa = 5.52 AEE305 pKa = 4.78 QEE307 pKa = 4.25 RR308 pKa = 11.84 QSQQQQEE315 pKa = 4.41 DD316 pKa = 3.41 EE317 pKa = 4.17 RR318 pKa = 11.84 MQRR321 pKa = 11.84 LGQRR325 pKa = 11.84 AEE327 pKa = 3.82 KK328 pKa = 10.63 CRR330 pKa = 11.84 GKK332 pKa = 9.53 MLLRR336 pKa = 11.84 KK337 pKa = 9.45 GLQHH341 pKa = 6.06 WFARR345 pKa = 11.84 HH346 pKa = 4.04 EE347 pKa = 4.05 VRR349 pKa = 11.84 RR350 pKa = 11.84 AEE352 pKa = 4.13 LQRR355 pKa = 11.84 ASKK358 pKa = 10.31 FLLAMRR364 pKa = 11.84 YY365 pKa = 7.15 FRR367 pKa = 11.84 RR368 pKa = 11.84 WKK370 pKa = 10.52 HH371 pKa = 5.06 IALEE375 pKa = 4.24 NASKK379 pKa = 10.66 ARR381 pKa = 11.84 SILTRR386 pKa = 11.84 KK387 pKa = 9.47 YY388 pKa = 8.63 IRR390 pKa = 11.84 VWRR393 pKa = 11.84 EE394 pKa = 3.13 RR395 pKa = 11.84 TARR398 pKa = 11.84 SHH400 pKa = 6.48 LAEE403 pKa = 4.27 EE404 pKa = 4.26 QAAAHH409 pKa = 6.43 HH410 pKa = 6.66 EE411 pKa = 4.33 EE412 pKa = 4.21 TLQRR416 pKa = 11.84 QCVKK420 pKa = 9.9 RR421 pKa = 11.84 WAKK424 pKa = 10.37 VLFNIKK430 pKa = 10.04 LEE432 pKa = 3.78 KK433 pKa = 10.3 RR434 pKa = 11.84 RR435 pKa = 11.84 IDD437 pKa = 4.08 RR438 pKa = 11.84 IKK440 pKa = 10.58 HH441 pKa = 4.42 AACRR445 pKa = 11.84 KK446 pKa = 9.04 WLDD449 pKa = 3.02 AVQRR453 pKa = 11.84 VRR455 pKa = 11.84 ALEE458 pKa = 4.03 AQAAAFRR465 pKa = 11.84 SQRR468 pKa = 11.84 LVRR471 pKa = 11.84 PVLLALRR478 pKa = 11.84 AGVLRR483 pKa = 11.84 VQDD486 pKa = 3.89 LASRR490 pKa = 11.84 AQTCYY495 pKa = 10.31 AQARR499 pKa = 11.84 LTIAFSVMRR508 pKa = 11.84 IQGRR512 pKa = 11.84 LKK514 pKa = 10.03 PLEE517 pKa = 3.92 NVMTLRR523 pKa = 11.84 LNLTLEE529 pKa = 4.38 RR530 pKa = 11.84 KK531 pKa = 9.65 AFATWHH537 pKa = 6.03 LQVQLAQQAAHH548 pKa = 6.68 FDD550 pKa = 3.23 QRR552 pKa = 11.84 RR553 pKa = 11.84 IKK555 pKa = 9.92 RR556 pKa = 11.84 AAWHH560 pKa = 6.0 GWVDD564 pKa = 3.43 ALKK567 pKa = 10.39 CRR569 pKa = 11.84 VVAQHH574 pKa = 5.58 INARR578 pKa = 11.84 VVIEE582 pKa = 4.18 SLYY585 pKa = 10.72 RR586 pKa = 11.84 WMLQEE591 pKa = 3.98 RR592 pKa = 11.84 LQLFRR597 pKa = 11.84 RR598 pKa = 11.84 TADD601 pKa = 3.02 ARR603 pKa = 11.84 LLRR606 pKa = 11.84 NSFAALKK613 pKa = 10.71 GMSTDD618 pKa = 3.74 LQAQLDD624 pKa = 3.7 MRR626 pKa = 11.84 AYY628 pKa = 10.59 AFAQGQQRR636 pKa = 11.84 RR637 pKa = 11.84 RR638 pKa = 11.84 LAGAMFRR645 pKa = 11.84 LHH647 pKa = 5.37 VLSRR651 pKa = 11.84 RR652 pKa = 11.84 RR653 pKa = 11.84 EE654 pKa = 3.97 DD655 pKa = 3.26 AEE657 pKa = 3.88 RR658 pKa = 11.84 AAVEE662 pKa = 4.24 FANSRR667 pKa = 11.84 ALPAVLEE674 pKa = 4.36 TWHH677 pKa = 6.79 EE678 pKa = 4.32 KK679 pKa = 6.36 TTHH682 pKa = 4.0 VTKK685 pKa = 10.82 LNRR688 pKa = 11.84 WATDD692 pKa = 2.64 ARR694 pKa = 11.84 FYY696 pKa = 10.45 CLCSAALKK704 pKa = 9.68 RR705 pKa = 11.84 WRR707 pKa = 11.84 DD708 pKa = 3.26 ATVEE712 pKa = 4.08 HH713 pKa = 5.09 QHH715 pKa = 5.02 QRR717 pKa = 11.84 RR718 pKa = 11.84 RR719 pKa = 11.84 DD720 pKa = 3.11 AYY722 pKa = 8.04 AQVRR726 pKa = 11.84 SRR728 pKa = 11.84 YY729 pKa = 7.5 KK730 pKa = 10.12 HH731 pKa = 6.23 RR732 pKa = 11.84 LVGQCFASLQVKK744 pKa = 8.82 YY745 pKa = 11.07 AEE747 pKa = 4.49 VYY749 pKa = 10.82 SMSAAIQDD757 pKa = 3.81 RR758 pKa = 11.84 AQTTQTTTVAQLFATWRR775 pKa = 11.84 SRR777 pKa = 11.84 ARR779 pKa = 11.84 TLQAQSLTASQLDD792 pKa = 3.75 TSRR795 pKa = 11.84 LLSSALSALTSQHH808 pKa = 6.09 AQTTSLDD815 pKa = 3.69 TEE817 pKa = 4.43 AEE819 pKa = 4.21 TFARR823 pKa = 11.84 TTSLSLALSALKK835 pKa = 9.26 TLQWATFTASQQSHH849 pKa = 5.21 TALALRR855 pKa = 11.84 SRR857 pKa = 11.84 NRR859 pKa = 11.84 EE860 pKa = 3.29 QHH862 pKa = 5.03 IRR864 pKa = 11.84 HH865 pKa = 5.98 MLRR868 pKa = 11.84 HH869 pKa = 5.53 WVSQTAARR877 pKa = 11.84 RR878 pKa = 11.84 AAATAEE884 pKa = 3.95 PDD886 pKa = 3.49 AEE888 pKa = 4.51 LEE890 pKa = 4.42 AEE892 pKa = 4.35 PEE894 pKa = 4.4 SPSLRR899 pKa = 11.84 PASRR903 pKa = 11.84 AAARR907 pKa = 11.84 SLSRR911 pKa = 11.84 DD912 pKa = 3.69 RR913 pKa = 11.84 IRR915 pKa = 11.84 DD916 pKa = 3.37 RR917 pKa = 11.84 YY918 pKa = 8.4 TSPTRR923 pKa = 11.84 LPQGYY928 pKa = 9.96 AGSTPGYY935 pKa = 9.68 LRR937 pKa = 11.84 TPSRR941 pKa = 11.84 SRR943 pKa = 11.84 RR944 pKa = 11.84 AVGRR948 pKa = 11.84 FRR950 pKa = 11.84 PIPTPGPYY958 pKa = 9.31 TPLAFTPAYY967 pKa = 9.8 LVTGIPGVQVDD978 pKa = 4.9 DD979 pKa = 4.55 GAGLDD984 pKa = 4.01 MGSSPPLPMALAGSGQVTPFAQRR1007 pKa = 11.84 LRR1009 pKa = 11.84 EE1010 pKa = 4.11 VGVVEE1015 pKa = 5.68 AIPTGAITGAGAEE1028 pKa = 4.0 FRR1030 pKa = 11.84 PVSVLRR1036 pKa = 11.84 RR1037 pKa = 11.84 GLRR1040 pKa = 11.84 QSVTGGTGTGKK1051 pKa = 9.69 SVRR1054 pKa = 11.84 FAGAGRR1060 pKa = 11.84 FSSVGGGSRR1069 pKa = 11.84 GQEE1072 pKa = 3.91 HH1073 pKa = 6.94 LKK1075 pKa = 10.97 SSS1077 pKa = 3.56
Molecular weight: 122.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.545
IPC_protein 10.511
Toseland 10.672
ProMoST 10.467
Dawson 10.76
Bjellqvist 10.526
Wikipedia 11.008
Rodwell 10.833
Grimsley 10.818
Solomon 10.921
Lehninger 10.877
Nozaki 10.672
DTASelect 10.511
Thurlkill 10.672
EMBOSS 11.096
Sillero 10.701
Patrickios 10.482
IPC_peptide 10.935
IPC2_peptide 9.633
IPC2.peptide.svr19 8.72
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18598
0
18598
9390076
50
6082
504.9
55.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.694 ± 0.016
1.083 ± 0.006
5.769 ± 0.015
6.108 ± 0.017
3.367 ± 0.012
7.231 ± 0.018
2.384 ± 0.008
4.44 ± 0.014
4.701 ± 0.015
8.719 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.202 ± 0.008
3.33 ± 0.01
6.047 ± 0.019
4.04 ± 0.013
6.198 ± 0.018
8.127 ± 0.018
6.293 ± 0.014
6.209 ± 0.013
1.392 ± 0.006
2.668 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here