Bamboo mosaic virus satellite RNA
Average proteome isoelectric point is 8.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6XXN6|Q6XXN6_9VIRU Coat protein OS=Bamboo mosaic virus satellite RNA OX=190811 GN=P20 PE=4 SV=1
MM1 pKa = 6.79 VRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 NRR7 pKa = 11.84 RR8 pKa = 11.84 QRR10 pKa = 11.84 SRR12 pKa = 11.84 VSQMTDD18 pKa = 1.81 IMYY21 pKa = 10.83 GSLTLGSTTTWTRR34 pKa = 11.84 KK35 pKa = 9.21 NFPGLANMGDD45 pKa = 3.58 RR46 pKa = 11.84 PFQVISVKK54 pKa = 10.35 IVVSSASPMLYY65 pKa = 9.52 QARR68 pKa = 11.84 LYY70 pKa = 10.85 SPHH73 pKa = 7.69 DD74 pKa = 4.42 DD75 pKa = 4.3 DD76 pKa = 5.75 NVGSTGLQMSGTTPHH91 pKa = 5.55 THH93 pKa = 6.59 HH94 pKa = 6.31 MRR96 pKa = 11.84 ALPGQNTWFSGNTSSTQVIVAIDD119 pKa = 3.65 GLKK122 pKa = 9.87 TKK124 pKa = 8.21 TTDD127 pKa = 3.01 ATPQNAVAVQVFYY140 pKa = 10.92 RR141 pKa = 11.84 VAPSEE146 pKa = 4.11 LQSATGNAEE155 pKa = 4.09 MPTTTPFDD163 pKa = 3.67 LPEE166 pKa = 5.73 GYY168 pKa = 10.09 EE169 pKa = 4.01 YY170 pKa = 11.04 LADD173 pKa = 3.19 AWLPDD178 pKa = 3.72 RR179 pKa = 11.84 APTSS183 pKa = 3.45
Molecular weight: 20.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.831
IPC2_protein 8.946
IPC_protein 9.18
Toseland 9.282
ProMoST 9.224
Dawson 9.633
Bjellqvist 9.385
Wikipedia 9.882
Rodwell 9.692
Grimsley 9.75
Solomon 9.736
Lehninger 9.677
Nozaki 9.224
DTASelect 9.399
Thurlkill 9.414
EMBOSS 9.721
Sillero 9.531
Patrickios 4.8
IPC_peptide 9.721
IPC2_peptide 8.141
IPC2.peptide.svr19 7.836
Protein with the highest isoelectric point:
>tr|Q6XXN6|Q6XXN6_9VIRU Coat protein OS=Bamboo mosaic virus satellite RNA OX=190811 GN=P20 PE=4 SV=1
MM1 pKa = 6.79 VRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 NRR7 pKa = 11.84 RR8 pKa = 11.84 QRR10 pKa = 11.84 SRR12 pKa = 11.84 VSQMTDD18 pKa = 1.81 IMYY21 pKa = 10.83 GSLTLGSTTTWTRR34 pKa = 11.84 KK35 pKa = 9.21 NFPGLANMGDD45 pKa = 3.58 RR46 pKa = 11.84 PFQVISVKK54 pKa = 10.35 IVVSSASPMLYY65 pKa = 9.52 QARR68 pKa = 11.84 LYY70 pKa = 10.85 SPHH73 pKa = 7.69 DD74 pKa = 4.42 DD75 pKa = 4.3 DD76 pKa = 5.75 NVGSTGLQMSGTTPHH91 pKa = 5.55 THH93 pKa = 6.59 HH94 pKa = 6.31 MRR96 pKa = 11.84 ALPGQNTWFSGNTSSTQVIVAIDD119 pKa = 3.65 GLKK122 pKa = 9.87 TKK124 pKa = 8.21 TTDD127 pKa = 3.01 ATPQNAVAVQVFYY140 pKa = 10.92 RR141 pKa = 11.84 VAPSEE146 pKa = 4.11 LQSATGNAEE155 pKa = 4.09 MPTTTPFDD163 pKa = 3.67 LPEE166 pKa = 5.73 GYY168 pKa = 10.09 EE169 pKa = 4.01 YY170 pKa = 11.04 LADD173 pKa = 3.19 AWLPDD178 pKa = 3.72 RR179 pKa = 11.84 APTSS183 pKa = 3.45
Molecular weight: 20.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.831
IPC2_protein 8.946
IPC_protein 9.18
Toseland 9.282
ProMoST 9.224
Dawson 9.633
Bjellqvist 9.385
Wikipedia 9.882
Rodwell 9.692
Grimsley 9.75
Solomon 9.736
Lehninger 9.677
Nozaki 9.224
DTASelect 9.399
Thurlkill 9.414
EMBOSS 9.721
Sillero 9.531
Patrickios 4.8
IPC_peptide 9.721
IPC2_peptide 8.141
IPC2.peptide.svr19 7.836
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
183
183
183
183.0
20.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.65 ± 0.0
0.0 ± 0.0
5.464 ± 0.0
2.186 ± 0.0
2.732 ± 0.0
6.557 ± 0.0
2.186 ± 0.0
2.732 ± 0.0
2.186 ± 0.0
6.557 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.372 ± 0.0
4.372 ± 0.0
7.104 ± 0.0
5.464 ± 0.0
7.104 ± 0.0
9.29 ± 0.0
12.022 ± 0.0
7.104 ± 0.0
1.639 ± 0.0
3.279 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here