Lactococcus phage 936 group phage Phi129

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; unclassified Skunavirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126HAE5|A0A126HAE5_9CAUD Portal protein OS=Lactococcus phage 936 group phage Phi129 OX=1636558 GN=Phi129_04 PE=4 SV=1
MM1 pKa = 7.56NEE3 pKa = 3.62FDD5 pKa = 6.3SYY7 pKa = 11.39IDD9 pKa = 3.33WYY11 pKa = 11.58NNLLTMPLNDD21 pKa = 4.53IILGVKK27 pKa = 8.95DD28 pKa = 3.74TIEE31 pKa = 4.55DD32 pKa = 3.52KK33 pKa = 10.67TVYY36 pKa = 10.64LSLDD40 pKa = 3.37DD41 pKa = 4.12SKK43 pKa = 11.43VIKK46 pKa = 9.75MDD48 pKa = 3.07NTSFVMGYY56 pKa = 9.13YY57 pKa = 8.74YY58 pKa = 10.45QVVLSVKK65 pKa = 10.52DD66 pKa = 3.48VDD68 pKa = 4.31DD69 pKa = 4.13EE70 pKa = 4.3LVRR73 pKa = 11.84LVGDD77 pKa = 3.57VLRR80 pKa = 11.84DD81 pKa = 3.4GWNMTNWSEE90 pKa = 4.32NSHH93 pKa = 6.58LYY95 pKa = 10.7NYY97 pKa = 8.61TGTVYY102 pKa = 10.42LPCGAGGQPWQQ113 pKa = 3.69

Molecular weight: 13.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126HAH5|A0A126HAH5_9CAUD Uncharacterized protein OS=Lactococcus phage 936 group phage Phi129 OX=1636558 GN=Phi129_34 PE=4 SV=1
MM1 pKa = 7.46CKK3 pKa = 9.75KK4 pKa = 10.38RR5 pKa = 11.84KK6 pKa = 5.78YY7 pKa = 8.56TKK9 pKa = 9.78MGALYY14 pKa = 10.54SIANAQHH21 pKa = 6.45RR22 pKa = 11.84KK23 pKa = 9.78KK24 pKa = 10.55KK25 pKa = 10.0GDD27 pKa = 3.95KK28 pKa = 9.53IPVRR32 pKa = 11.84AYY34 pKa = 9.48HH35 pKa = 6.61CKK37 pKa = 8.93WCNLYY42 pKa = 10.43HH43 pKa = 7.32LSSQQRR49 pKa = 11.84LNIKK53 pKa = 9.21TGVIGG58 pKa = 3.97

Molecular weight: 6.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

8792

33

999

162.8

18.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.438 ± 0.661

0.626 ± 0.146

5.573 ± 0.269

7.291 ± 0.608

4.163 ± 0.274

6.631 ± 0.501

1.285 ± 0.206

7.063 ± 0.281

9.042 ± 0.478

8.701 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.158

6.665 ± 0.311

2.172 ± 0.268

3.753 ± 0.243

3.401 ± 0.275

6.404 ± 0.548

6.506 ± 0.404

6.358 ± 0.378

1.558 ± 0.163

3.981 ± 0.446
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski