Synechococcus phage metaG-MbCM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H8ZNK7|H8ZNK7_9CAUD Uncharacterized protein OS=Synechococcus phage metaG-MbCM1 OX=1079999 PE=4 SV=1
MM1 pKa = 7.67LIALATLVTIGAFILGITVSWLAKK25 pKa = 10.34GYY27 pKa = 10.55VEE29 pKa = 6.23DD30 pKa = 4.57YY31 pKa = 10.93IEE33 pKa = 4.03NAAYY37 pKa = 9.89AKK39 pKa = 10.38SVTHH43 pKa = 7.03PEE45 pKa = 3.98MLDD48 pKa = 3.18EE49 pKa = 5.86DD50 pKa = 5.06GNILHH55 pKa = 7.6DD56 pKa = 3.72EE57 pKa = 4.81LIYY60 pKa = 10.83VRR62 pKa = 11.84PTSPWDD68 pKa = 3.37IQDD71 pKa = 4.55LDD73 pKa = 4.7DD74 pKa = 6.13DD75 pKa = 4.71EE76 pKa = 6.24EE77 pKa = 4.46DD78 pKa = 3.36

Molecular weight: 8.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H8ZN64|H8ZN64_9CAUD Uncharacterized protein OS=Synechococcus phage metaG-MbCM1 OX=1079999 PE=4 SV=1
MM1 pKa = 7.64LLNIKK6 pKa = 10.09INLLLLTIPRR16 pKa = 11.84LISQVIVLLARR27 pKa = 11.84NLPIVNDD34 pKa = 3.83SQQTQIFLTDD44 pKa = 3.86YY45 pKa = 10.33QQLWYY50 pKa = 9.86NNSVRR55 pKa = 11.84VQRR58 pKa = 11.84FLL60 pKa = 3.94

Molecular weight: 7.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

234

0

234

52596

30

2409

224.8

24.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.004 ± 0.211

0.913 ± 0.079

6.548 ± 0.119

5.727 ± 0.218

4.297 ± 0.103

7.879 ± 0.288

1.538 ± 0.098

5.995 ± 0.154

5.805 ± 0.312

7.172 ± 0.172

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.205 ± 0.167

5.864 ± 0.16

3.862 ± 0.164

3.793 ± 0.109

3.9 ± 0.145

7.312 ± 0.22

7.976 ± 0.393

6.888 ± 0.17

1.078 ± 0.084

4.246 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski