Prevotella sp. S7-1-8

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; unclassified Prevotella

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2240 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A096AAM4|A0A096AAM4_9BACT Uncharacterized protein OS=Prevotella sp. S7-1-8 OX=1284775 GN=HMPREF1640_05260 PE=4 SV=1
MM1 pKa = 7.68TYY3 pKa = 9.77FCEE6 pKa = 3.75SHH8 pKa = 6.59EE9 pKa = 4.2YY10 pKa = 10.37VRR12 pKa = 11.84VEE14 pKa = 4.41GEE16 pKa = 3.59FGYY19 pKa = 10.32IGITDD24 pKa = 3.95YY25 pKa = 11.29AQKK28 pKa = 11.21ALGNIVYY35 pKa = 10.47VDD37 pKa = 3.88MPDD40 pKa = 3.48VDD42 pKa = 5.43DD43 pKa = 5.0EE44 pKa = 4.8LSAGDD49 pKa = 4.05DD50 pKa = 3.83FGAVEE55 pKa = 4.38SVKK58 pKa = 10.64AASDD62 pKa = 3.21LTAPVSGVVVEE73 pKa = 4.31VNEE76 pKa = 4.1ALEE79 pKa = 4.88DD80 pKa = 3.89EE81 pKa = 4.62PALINKK87 pKa = 7.77DD88 pKa = 4.2ANANWIIKK96 pKa = 10.0VKK98 pKa = 9.74LTDD101 pKa = 3.9PSEE104 pKa = 4.48LEE106 pKa = 3.82NLMSEE111 pKa = 4.45EE112 pKa = 5.07DD113 pKa = 3.84YY114 pKa = 11.11LASVQQ119 pKa = 3.96

Molecular weight: 13.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095Y6L4|A0A095Y6L4_9BACT Succinate--CoA ligase [ADP-forming] subunit alpha OS=Prevotella sp. S7-1-8 OX=1284775 GN=sucD PE=3 SV=1
MM1 pKa = 7.38ARR3 pKa = 11.84VALGVPRR10 pKa = 11.84YY11 pKa = 10.39EE12 pKa = 3.76MTAPKK17 pKa = 10.1NGRR20 pKa = 11.84DD21 pKa = 3.56HH22 pKa = 6.42TWRR25 pKa = 11.84VCVATFVAYY34 pKa = 9.32AAPFATCLRR43 pKa = 11.84RR44 pKa = 11.84EE45 pKa = 4.15LAVVRR50 pKa = 11.84AAYY53 pKa = 9.84RR54 pKa = 11.84RR55 pKa = 11.84SEE57 pKa = 4.02EE58 pKa = 4.09NMLKK62 pKa = 10.59NSDD65 pKa = 3.44KK66 pKa = 10.91SLSVLCVFANGGRR79 pKa = 11.84LGRR82 pKa = 11.84KK83 pKa = 7.73FVKK86 pKa = 10.66NGVVLL91 pKa = 3.99

Molecular weight: 10.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2240

0

2240

746712

42

2563

333.4

37.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.742 ± 0.055

1.308 ± 0.019

5.889 ± 0.038

5.918 ± 0.058

4.347 ± 0.035

6.83 ± 0.052

2.094 ± 0.023

6.351 ± 0.043

6.708 ± 0.047

8.728 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.031 ± 0.026

5.011 ± 0.046

3.653 ± 0.024

3.532 ± 0.036

5.448 ± 0.042

5.74 ± 0.043

5.569 ± 0.035

6.773 ± 0.049

1.205 ± 0.018

4.125 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski