Bovine adenovirus 2 (BAdV-2) (Mastadenovirus bos2)
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9EEG3|Q9EEG3_ADEB2 Uncharacterized protein OS=Bovine adenovirus 2 OX=114429 PE=4 SV=1
MM1 pKa = 7.46 RR2 pKa = 11.84 HH3 pKa = 5.85 LRR5 pKa = 11.84 LAFDD9 pKa = 3.22 WRR11 pKa = 11.84 FWEE14 pKa = 4.17 AAEE17 pKa = 5.16 NLLQDD22 pKa = 3.86 LHH24 pKa = 6.74 TSEE27 pKa = 6.25 DD28 pKa = 3.66 EE29 pKa = 4.41 DD30 pKa = 3.91 EE31 pKa = 5.15 GFSEE35 pKa = 5.07 PLTLQDD41 pKa = 4.24 LVEE44 pKa = 4.58 LEE46 pKa = 4.42 SPNAVDD52 pKa = 5.19 YY53 pKa = 8.16 FFPDD57 pKa = 2.92 ADD59 pKa = 4.04 IPQEE63 pKa = 4.2 LPTDD67 pKa = 3.86 AAAEE71 pKa = 4.0 EE72 pKa = 4.43 LLGCDD77 pKa = 3.64 EE78 pKa = 4.56 VVDD81 pKa = 5.33 LSTLFDD87 pKa = 4.09 SEE89 pKa = 5.41 AEE91 pKa = 4.24 STPNSPFSAVEE102 pKa = 3.53 LDD104 pKa = 3.97 YY105 pKa = 11.21 PEE107 pKa = 5.72 HH108 pKa = 7.03 PGHH111 pKa = 7.19 DD112 pKa = 3.88 CSACDD117 pKa = 3.36 YY118 pKa = 10.73 HH119 pKa = 8.85 RR120 pKa = 11.84 RR121 pKa = 11.84 ATKK124 pKa = 10.82 NEE126 pKa = 3.75 DD127 pKa = 3.82 TLCSLCYY134 pKa = 9.66 MRR136 pKa = 11.84 QNAFAVYY143 pKa = 9.92 GKK145 pKa = 10.59
Molecular weight: 16.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.973
IPC_protein 3.948
Toseland 3.757
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.821
Rodwell 3.77
Grimsley 3.668
Solomon 3.91
Lehninger 3.859
Nozaki 4.024
DTASelect 4.215
Thurlkill 3.783
EMBOSS 3.834
Sillero 4.062
Patrickios 1.138
IPC_peptide 3.91
IPC2_peptide 4.05
IPC2.peptide.svr19 3.944
Protein with the highest isoelectric point:
>sp|Q89592|CAP8_ADEB2 Pre-hexon-linking protein VIII OS=Bovine adenovirus 2 OX=114429 GN=L4 PE=3 SV=2
MM1 pKa = 7.51 TFAQDD6 pKa = 2.97 EE7 pKa = 4.38 ALIQTTATNYY17 pKa = 8.56 MLLYY21 pKa = 10.37 VIIFMMLKK29 pKa = 9.71 VKK31 pKa = 9.25 KK32 pKa = 8.75 TFYY35 pKa = 10.74 LWLFVVGVCGDD46 pKa = 2.82 IHH48 pKa = 7.52 ISKK51 pKa = 10.49 FEE53 pKa = 3.99 HH54 pKa = 7.05 EE55 pKa = 4.31 YY56 pKa = 11.23 AQLDD60 pKa = 3.96 FNATNWTEE68 pKa = 3.87 ASWYY72 pKa = 10.21 KK73 pKa = 10.85 LYY75 pKa = 10.16 TGKK78 pKa = 10.54 IINFGANLGNVNKK91 pKa = 10.47 NKK93 pKa = 9.74 ACRR96 pKa = 11.84 VFRR99 pKa = 11.84 KK100 pKa = 9.94
Molecular weight: 11.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.607
IPC2_protein 8.492
IPC_protein 8.507
Toseland 8.96
ProMoST 9.048
Dawson 9.268
Bjellqvist 9.151
Wikipedia 9.428
Rodwell 9.414
Grimsley 9.282
Solomon 9.428
Lehninger 9.385
Nozaki 9.224
DTASelect 9.048
Thurlkill 9.136
EMBOSS 9.399
Sillero 9.311
Patrickios 4.202
IPC_peptide 9.414
IPC2_peptide 7.907
IPC2.peptide.svr19 7.749
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
3019
63
1017
274.5
31.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.055 ± 0.471
2.749 ± 0.585
4.737 ± 0.581
6.691 ± 0.494
5.399 ± 0.407
4.969 ± 0.722
2.517 ± 0.314
6.028 ± 0.621
4.803 ± 0.936
9.573 ± 0.551
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.954 ± 0.236
5.2 ± 0.364
4.737 ± 0.528
4.439 ± 0.578
5.73 ± 0.861
7.022 ± 0.706
5.631 ± 0.439
5.697 ± 0.502
1.491 ± 0.249
3.577 ± 0.304
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here