Gremmeniella abietina RNA virus L1
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q99AT4|Q99AT4_9VIRU Putative coat protein OS=Gremmeniella abietina RNA virus L1 OX=152217 PE=4 SV=1
MM1 pKa = 7.45 ANAYY5 pKa = 7.02 EE6 pKa = 4.69 TNFQTANASPLTGTLADD23 pKa = 4.05 PVGGTIQADD32 pKa = 3.52 HH33 pKa = 6.03 YY34 pKa = 9.81 RR35 pKa = 11.84 RR36 pKa = 11.84 YY37 pKa = 9.59 RR38 pKa = 11.84 AGVFMSVPDD47 pKa = 3.77 MGHH50 pKa = 5.47 VSNVGRR56 pKa = 11.84 SIFYY60 pKa = 10.17 EE61 pKa = 4.15 VGRR64 pKa = 11.84 HH65 pKa = 5.22 HH66 pKa = 7.26 GRR68 pKa = 11.84 ARR70 pKa = 11.84 DD71 pKa = 3.8 LLAAPHH77 pKa = 6.81 AEE79 pKa = 5.06 AIPIDD84 pKa = 3.98 CSVDD88 pKa = 2.99 INAAEE93 pKa = 4.02 AANFEE98 pKa = 4.05 GMARR102 pKa = 11.84 RR103 pKa = 11.84 FSNFSPQWVKK113 pKa = 10.4 MDD115 pKa = 4.12 LAGMVEE121 pKa = 4.37 RR122 pKa = 11.84 LAKK125 pKa = 10.19 GVTAAGVFGGIDD137 pKa = 3.16 TGTLRR142 pKa = 11.84 GGQAIRR148 pKa = 11.84 VTALGTLDD156 pKa = 3.78 SPQTASINSVFIPRR170 pKa = 11.84 TVDD173 pKa = 3.11 TVGNDD178 pKa = 2.9 HH179 pKa = 6.29 VFAVLVSAANGEE191 pKa = 4.42 GAAVTTDD198 pKa = 3.57 VVRR201 pKa = 11.84 LDD203 pKa = 4.14 ANTNQPMIPAVAGASFATACVEE225 pKa = 3.88 ALRR228 pKa = 11.84 ILGANMEE235 pKa = 4.31 ASGAGDD241 pKa = 3.24 VFAYY245 pKa = 10.27 AVTRR249 pKa = 11.84 GVHH252 pKa = 5.91 AGVSVVAHH260 pKa = 6.03 TDD262 pKa = 2.89 EE263 pKa = 5.22 GGWFRR268 pKa = 11.84 NVLRR272 pKa = 11.84 HH273 pKa = 6.18 DD274 pKa = 4.3 RR275 pKa = 11.84 FRR277 pKa = 11.84 VPYY280 pKa = 10.4 GGINQGLRR288 pKa = 11.84 QYY290 pKa = 10.22 PALPALASTSPSSIAAWADD309 pKa = 3.77 AIALKK314 pKa = 9.18 TAAAVAHH321 pKa = 6.94 CDD323 pKa = 3.43 PLVPGEE329 pKa = 4.32 GGWYY333 pKa = 9.51 PSVFTAGQNGTSAPGSSEE351 pKa = 3.86 SAEE354 pKa = 4.19 VGDD357 pKa = 4.29 QEE359 pKa = 4.2 ARR361 pKa = 11.84 SIGRR365 pKa = 11.84 QIASDD370 pKa = 3.27 IGRR373 pKa = 11.84 FAPAYY378 pKa = 9.78 CRR380 pKa = 11.84 ALGTLFGLHH389 pKa = 5.92 TSSGVAEE396 pKa = 4.25 SHH398 pKa = 6.63 LSTVAMRR405 pKa = 11.84 ALTQDD410 pKa = 3.36 VNTDD414 pKa = 2.42 RR415 pKa = 11.84 HH416 pKa = 5.8 LRR418 pKa = 11.84 HH419 pKa = 5.81 EE420 pKa = 4.46 TVAPYY425 pKa = 9.78 FWIEE429 pKa = 3.92 PTSLIAVNAFGTPAEE444 pKa = 4.19 AEE446 pKa = 4.7 GYY448 pKa = 9.32 GSKK451 pKa = 8.79 VTPGDD456 pKa = 3.66 TRR458 pKa = 11.84 SEE460 pKa = 4.06 PCFEE464 pKa = 4.83 RR465 pKa = 11.84 FRR467 pKa = 11.84 LLQKK471 pKa = 10.93 GSTANHH477 pKa = 4.52 MTVAFKK483 pKa = 10.39 MRR485 pKa = 11.84 TARR488 pKa = 11.84 TSALVSAYY496 pKa = 9.94 AAAPAPLADD505 pKa = 3.76 LKK507 pKa = 11.05 LYY509 pKa = 10.7 QFDD512 pKa = 3.78 EE513 pKa = 4.94 SSVVLPGDD521 pKa = 3.47 QLPTQGDD528 pKa = 3.99 VAHH531 pKa = 6.79 KK532 pKa = 9.99 HH533 pKa = 5.43 AAADD537 pKa = 3.92 PLSSYY542 pKa = 9.43 LWKK545 pKa = 10.49 RR546 pKa = 11.84 GQSCFPAPAEE556 pKa = 4.37 FINTNSNYY564 pKa = 8.64 GAKK567 pKa = 9.86 VRR569 pKa = 11.84 LVTWDD574 pKa = 3.8 DD575 pKa = 4.0 DD576 pKa = 4.65 FNASLSDD583 pKa = 3.74 LPNDD587 pKa = 3.41 EE588 pKa = 4.52 EE589 pKa = 4.39 MATGVVTFRR598 pKa = 11.84 VTVPTGLASAGSNAEE613 pKa = 3.59 NRR615 pKa = 11.84 EE616 pKa = 3.94 AKK618 pKa = 10.05 RR619 pKa = 11.84 ARR621 pKa = 11.84 TRR623 pKa = 11.84 GAIALAQAVLRR634 pKa = 11.84 ARR636 pKa = 11.84 ATGLAVSPSMEE647 pKa = 4.02 ISDD650 pKa = 4.17 VPPTFDD656 pKa = 4.34 TPPLLPAPTYY666 pKa = 11.19 SDD668 pKa = 3.52 TQSASGPRR676 pKa = 11.84 DD677 pKa = 3.64 PGPVPVLAVGGGLDD691 pKa = 3.22 VGRR694 pKa = 11.84 VARR697 pKa = 11.84 GAPLPPTAHH706 pKa = 6.87 HH707 pKa = 6.25 MPQRR711 pKa = 11.84 APRR714 pKa = 11.84 IAGAVVPPGGPVAGPPAATGGPAAPNVPPPPPAGPLDD751 pKa = 4.01 SQDD754 pKa = 4.39 PLPAPPAHH762 pKa = 6.62 LPAEE766 pKa = 4.36 ATAAEE771 pKa = 4.39 AVPAQQ776 pKa = 3.62
Molecular weight: 80.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.732
IPC2_protein 5.588
IPC_protein 5.652
Toseland 6.122
ProMoST 5.995
Dawson 5.919
Bjellqvist 5.931
Wikipedia 5.931
Rodwell 5.906
Grimsley 6.313
Solomon 5.919
Lehninger 5.906
Nozaki 6.186
DTASelect 6.376
Thurlkill 6.389
EMBOSS 6.351
Sillero 6.287
Patrickios 4.24
IPC_peptide 5.931
IPC2_peptide 6.3
IPC2.peptide.svr19 6.189
Protein with the highest isoelectric point:
>tr|Q99AT4|Q99AT4_9VIRU Putative coat protein OS=Gremmeniella abietina RNA virus L1 OX=152217 PE=4 SV=1
MM1 pKa = 7.53 IEE3 pKa = 4.23 SPVSVRR9 pKa = 11.84 ANEE12 pKa = 3.86 AGIVGQYY19 pKa = 11.03 LKK21 pKa = 11.21 GLLNMEE27 pKa = 4.36 WASGVMVLSFSQQISEE43 pKa = 4.43 VYY45 pKa = 10.21 KK46 pKa = 9.26 PTFNGLRR53 pKa = 11.84 PTDD56 pKa = 3.86 LQRR59 pKa = 11.84 AAAAYY64 pKa = 7.87 LVPDD68 pKa = 4.48 FPVQVRR74 pKa = 11.84 IEE76 pKa = 4.17 RR77 pKa = 11.84 GSVLSLLSQVIDD89 pKa = 3.9 PPARR93 pKa = 11.84 VTDD96 pKa = 3.48 RR97 pKa = 11.84 CSFRR101 pKa = 11.84 WLCDD105 pKa = 3.48 PKK107 pKa = 11.01 ASYY110 pKa = 10.66 AAFPPKK116 pKa = 9.91 QHH118 pKa = 6.89 PGAMNKK124 pKa = 9.3 VNVYY128 pKa = 9.74 LNEE131 pKa = 4.12 VASSLLSLDD140 pKa = 3.34 PAAYY144 pKa = 10.2 SSASQALWPHH154 pKa = 6.4 KK155 pKa = 10.68 GKK157 pKa = 10.17 IANDD161 pKa = 3.31 QASAIILYY169 pKa = 10.25 GYY171 pKa = 8.74 GLRR174 pKa = 11.84 AQAVPDD180 pKa = 3.87 AMHH183 pKa = 6.32 VAATLATTPDD193 pKa = 3.35 LAKK196 pKa = 10.91 ALTNFLKK203 pKa = 10.29 ATGANGSRR211 pKa = 11.84 LGALLCEE218 pKa = 4.72 SNVLLGRR225 pKa = 11.84 AAGPADD231 pKa = 3.67 LSEE234 pKa = 4.07 EE235 pKa = 3.73 ARR237 pKa = 11.84 YY238 pKa = 7.94 RR239 pKa = 11.84 TSSDD243 pKa = 3.63 VEE245 pKa = 3.92 SHH247 pKa = 6.78 LAIFSDD253 pKa = 3.9 ADD255 pKa = 3.41 LGAAIDD261 pKa = 4.82 AILDD265 pKa = 3.74 EE266 pKa = 4.81 EE267 pKa = 4.51 IKK269 pKa = 10.59 RR270 pKa = 11.84 VEE272 pKa = 4.12 GSQHH276 pKa = 6.93 IEE278 pKa = 3.42 FDD280 pKa = 3.77 SYY282 pKa = 10.99 QEE284 pKa = 3.82 HH285 pKa = 6.96 WNDD288 pKa = 2.68 RR289 pKa = 11.84 WAWAVNGAHH298 pKa = 6.48 SGHH301 pKa = 6.12 VSRR304 pKa = 11.84 LYY306 pKa = 10.33 PRR308 pKa = 11.84 VPKK311 pKa = 10.14 PPGMLRR317 pKa = 11.84 EE318 pKa = 4.16 HH319 pKa = 6.09 RR320 pKa = 11.84 RR321 pKa = 11.84 AWLEE325 pKa = 3.93 SVTEE329 pKa = 4.26 DD330 pKa = 5.17 PRR332 pKa = 11.84 TDD334 pKa = 3.01 WDD336 pKa = 3.47 GKK338 pKa = 7.95 TFVSASPKK346 pKa = 10.49 LEE348 pKa = 4.2 AGKK351 pKa = 8.26 TRR353 pKa = 11.84 AIFACDD359 pKa = 3.2 TVNYY363 pKa = 10.17 LAFEE367 pKa = 4.41 HH368 pKa = 6.8 LLAPVEE374 pKa = 4.2 KK375 pKa = 9.55 RR376 pKa = 11.84 WRR378 pKa = 11.84 NSKK381 pKa = 10.7 VILDD385 pKa = 4.09 PGRR388 pKa = 11.84 GGHH391 pKa = 6.75 LGMIFRR397 pKa = 11.84 TTAARR402 pKa = 11.84 ARR404 pKa = 11.84 AGVSMMLDD412 pKa = 3.12 YY413 pKa = 11.58 DD414 pKa = 4.48 DD415 pKa = 5.93 FNSHH419 pKa = 6.2 HH420 pKa = 6.27 STRR423 pKa = 11.84 AMQMLFQRR431 pKa = 11.84 LGDD434 pKa = 3.7 RR435 pKa = 11.84 VGYY438 pKa = 8.83 PADD441 pKa = 3.69 KK442 pKa = 10.33 LAKK445 pKa = 9.98 LVASFEE451 pKa = 3.91 KK452 pKa = 9.89 MYY454 pKa = 10.0 IYY456 pKa = 10.95 NGMEE460 pKa = 3.43 QVGRR464 pKa = 11.84 VKK466 pKa = 9.33 GTLMSGHH473 pKa = 7.16 RR474 pKa = 11.84 GTTFINSVLNKK485 pKa = 10.19 AYY487 pKa = 10.86 LLIVLGEE494 pKa = 4.19 DD495 pKa = 3.0 LFEE498 pKa = 5.48 RR499 pKa = 11.84 SVALHH504 pKa = 6.33 VGDD507 pKa = 4.47 DD508 pKa = 3.84 VYY510 pKa = 11.4 FGVRR514 pKa = 11.84 TYY516 pKa = 11.59 AEE518 pKa = 4.12 AGEE521 pKa = 4.43 VVTRR525 pKa = 11.84 IKK527 pKa = 10.88 NSPLRR532 pKa = 11.84 MNRR535 pKa = 11.84 MKK537 pKa = 10.71 QSVGHH542 pKa = 6.0 VSTEE546 pKa = 3.74 FLRR549 pKa = 11.84 NATSGRR555 pKa = 11.84 STYY558 pKa = 10.86 GYY560 pKa = 9.41 FARR563 pKa = 11.84 AVASTVSGNWVNEE576 pKa = 4.02 MALSPSEE583 pKa = 4.05 ALSSIIGAARR593 pKa = 11.84 TLVNRR598 pKa = 11.84 SGAVNLPLLLHH609 pKa = 6.56 PSLVRR614 pKa = 11.84 MTGLPRR620 pKa = 11.84 EE621 pKa = 4.17 DD622 pKa = 3.23 HH623 pKa = 6.31 KK624 pKa = 11.39 KK625 pKa = 10.45 LRR627 pKa = 11.84 EE628 pKa = 4.04 LLLGTTALDD637 pKa = 3.57 NGPQYY642 pKa = 11.49 SLGGYY647 pKa = 6.23 YY648 pKa = 10.23 TSVSSLITTTASDD661 pKa = 3.17 RR662 pKa = 11.84 HH663 pKa = 6.49 GYY665 pKa = 7.63 TPLPRR670 pKa = 11.84 EE671 pKa = 3.83 ATTAYY676 pKa = 9.93 LSCAADD682 pKa = 3.59 PLEE685 pKa = 4.54 VNVLTQAGVSVVSAMEE701 pKa = 3.68 EE702 pKa = 3.73 ASFRR706 pKa = 11.84 KK707 pKa = 9.57 SLPARR712 pKa = 11.84 YY713 pKa = 7.55 TGYY716 pKa = 8.27 EE717 pKa = 4.09 TLRR720 pKa = 11.84 LGPKK724 pKa = 9.94 SLIRR728 pKa = 11.84 SIGTASVVDD737 pKa = 5.93 LISASPPRR745 pKa = 11.84 GVLEE749 pKa = 4.66 RR750 pKa = 11.84 YY751 pKa = 8.65 PLLTLAKK758 pKa = 9.7 RR759 pKa = 11.84 RR760 pKa = 11.84 LPEE763 pKa = 3.81 YY764 pKa = 9.59 LVRR767 pKa = 11.84 WAVSIAGGNPSAPDD781 pKa = 3.03 IGMEE785 pKa = 3.47 AWGEE789 pKa = 4.07 FKK791 pKa = 10.36 HH792 pKa = 6.83 GCMIATPMSYY802 pKa = 10.89 SDD804 pKa = 3.38 AATFGKK810 pKa = 8.94 RR811 pKa = 11.84 TVSTVLTCPLDD822 pKa = 3.43 AHH824 pKa = 6.15 VV825 pKa = 4.57
Molecular weight: 90.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.136
IPC2_protein 8.229
IPC_protein 8.141
Toseland 8.39
ProMoST 8.595
Dawson 8.902
Bjellqvist 8.931
Wikipedia 9.033
Rodwell 8.96
Grimsley 8.726
Solomon 8.975
Lehninger 8.931
Nozaki 8.931
DTASelect 8.799
Thurlkill 8.829
EMBOSS 9.033
Sillero 9.048
Patrickios 4.444
IPC_peptide 8.96
IPC2_peptide 7.746
IPC2.peptide.svr19 7.725
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1601
776
825
800.5
85.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.554 ± 1.778
0.812 ± 0.027
4.872 ± 0.197
4.435 ± 0.396
3.123 ± 0.248
8.307 ± 0.676
2.561 ± 0.011
3.248 ± 0.198
2.561 ± 0.796
8.62 ± 1.426
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.249 ± 0.309
3.435 ± 0.031
6.996 ± 1.23
2.498 ± 0.234
6.558 ± 0.349
7.808 ± 0.77
6.246 ± 0.406
7.995 ± 0.265
1.187 ± 0.198
2.936 ± 0.608
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here