Mycoavidus cysteinexigens
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2280 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z6ETB7|A0A2Z6ETB7_9BURK Ribonuclease PH OS=Mycoavidus cysteinexigens OX=1553431 GN=rph PE=3 SV=1
MM1 pKa = 6.82 TTYY4 pKa = 10.79 KK5 pKa = 10.88 YY6 pKa = 10.41 IVDD9 pKa = 3.96 EE10 pKa = 4.15 RR11 pKa = 11.84 WGFEE15 pKa = 4.08 DD16 pKa = 4.7 ADD18 pKa = 3.93 CFEE21 pKa = 5.25 SNWGEE26 pKa = 3.6 EE27 pKa = 3.97 DD28 pKa = 3.26 AEE30 pKa = 4.41 YY31 pKa = 10.73 VARR34 pKa = 11.84 EE35 pKa = 3.86 VAGNYY40 pKa = 9.17 YY41 pKa = 10.21 SEE43 pKa = 5.93 SEE45 pKa = 4.57 GTWEE49 pKa = 3.98 WNEE52 pKa = 4.3 SISIQIFKK60 pKa = 10.86 EE61 pKa = 4.15 DD62 pKa = 3.68 GTSLGVFEE70 pKa = 4.94 VGYY73 pKa = 10.61 DD74 pKa = 3.69 LEE76 pKa = 4.33 PQFWVFEE83 pKa = 4.28 EE84 pKa = 4.97 KK85 pKa = 10.12 PAQRR89 pKa = 11.84 SSPCC93 pKa = 3.52
Molecular weight: 10.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.935
IPC_protein 3.821
Toseland 3.656
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.643
Rodwell 3.656
Grimsley 3.579
Solomon 3.757
Lehninger 3.706
Nozaki 3.897
DTASelect 3.986
Thurlkill 3.681
EMBOSS 3.656
Sillero 3.935
Patrickios 0.846
IPC_peptide 3.757
IPC2_peptide 3.923
IPC2.peptide.svr19 3.85
Protein with the highest isoelectric point:
>tr|A0A2Z6ETU9|A0A2Z6ETU9_9BURK Ribonuclease 3 OS=Mycoavidus cysteinexigens OX=1553431 GN=rnc PE=3 SV=1
MM1 pKa = 7.51 NPRR4 pKa = 11.84 EE5 pKa = 4.23 TQGMLLGLLSVIIFSLTLPMTRR27 pKa = 11.84 IAIQDD32 pKa = 3.09 IHH34 pKa = 6.77 PLLNGLGRR42 pKa = 11.84 SLAAAIPAALLLAWRR57 pKa = 11.84 RR58 pKa = 11.84 EE59 pKa = 3.82 RR60 pKa = 11.84 WPTKK64 pKa = 8.31 QQFKK68 pKa = 10.71 GLAIVALGAIIVFPVCTAWAMRR90 pKa = 11.84 TLPASHH96 pKa = 6.36 GAIVNGLQPLCVAVYY111 pKa = 10.04 AVFFARR117 pKa = 11.84 EE118 pKa = 4.01 KK119 pKa = 9.89 PSRR122 pKa = 11.84 TFWLCSIGGSALILTYY138 pKa = 10.44 AIRR141 pKa = 11.84 AGGGTLMSGDD151 pKa = 3.64 IFMLIAVAFGGLGYY165 pKa = 9.31 TEE167 pKa = 4.81 GGRR170 pKa = 11.84 LAKK173 pKa = 10.49 QMGGWRR179 pKa = 11.84 VICWALVIAAPFLTLPVIWLAWLHH203 pKa = 5.92 TGPIALKK210 pKa = 9.03 SWLAFGYY217 pKa = 10.68 AAFFSQFIGLFIWYY231 pKa = 9.56 AGLALGGIARR241 pKa = 11.84 VSQVQQLQVFFTLAFSACFFGEE263 pKa = 4.06 RR264 pKa = 11.84 VGPDD268 pKa = 2.77 TWIFAAAVVVMLTLSSRR285 pKa = 11.84 TRR287 pKa = 11.84 IAALPPSSLTPQKK300 pKa = 10.2 IVHH303 pKa = 5.92 NN304 pKa = 4.43
Molecular weight: 32.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.692
IPC_protein 10.496
Toseland 10.482
ProMoST 10.233
Dawson 10.643
Bjellqvist 10.394
Wikipedia 10.862
Rodwell 10.818
Grimsley 10.701
Solomon 10.73
Lehninger 10.687
Nozaki 10.526
DTASelect 10.365
Thurlkill 10.526
EMBOSS 10.891
Sillero 10.57
Patrickios 10.526
IPC_peptide 10.73
IPC2_peptide 9.677
IPC2.peptide.svr19 8.403
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2280
0
2280
795496
43
2826
348.9
38.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.623 ± 0.079
0.951 ± 0.014
4.844 ± 0.031
6.034 ± 0.05
3.564 ± 0.035
6.829 ± 0.051
2.496 ± 0.023
5.484 ± 0.044
4.63 ± 0.05
11.037 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.104 ± 0.031
3.539 ± 0.035
4.392 ± 0.037
4.97 ± 0.045
5.994 ± 0.04
6.445 ± 0.08
5.362 ± 0.039
6.468 ± 0.041
1.472 ± 0.028
2.764 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here