Escherichia phage Lys8385Vzw

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3RZC4|A0A7G3RZC4_9CAUD Attachment invasion locus protein OS=Escherichia phage Lys8385Vzw OX=2724332 GN=Lys8385Vzw_20 PE=4 SV=1
MM1 pKa = 7.53TEE3 pKa = 3.73ADD5 pKa = 4.79LYY7 pKa = 10.56PHH9 pKa = 7.29LAHH12 pKa = 7.36LAGGQVYY19 pKa = 8.93PYY21 pKa = 10.5VVPLLDD27 pKa = 3.92GRR29 pKa = 11.84PSVALPWVVFSLISSVSADD48 pKa = 3.41VMGGQAEE55 pKa = 4.45SSVSVQIDD63 pKa = 3.74VYY65 pKa = 11.29AGTVTQARR73 pKa = 11.84QIRR76 pKa = 11.84QDD78 pKa = 3.04ARR80 pKa = 11.84EE81 pKa = 4.64AIMLLAPGSVSEE93 pKa = 4.15MQDD96 pKa = 3.45YY97 pKa = 10.69IPEE100 pKa = 4.16NRR102 pKa = 11.84CYY104 pKa = 10.4RR105 pKa = 11.84ATLEE109 pKa = 4.21FQVTVV114 pKa = 3.48

Molecular weight: 12.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3WCY4|A0A7G3WCY4_9CAUD DNA-binding protein OS=Escherichia phage Lys8385Vzw OX=2724332 GN=Lys8385Vzw_46 PE=4 SV=1
MM1 pKa = 7.28IRR3 pKa = 11.84NIFKK7 pKa = 10.82RR8 pKa = 11.84FTNQTFRR15 pKa = 11.84CPRR18 pKa = 11.84PGQWYY23 pKa = 5.47TTPAGHH29 pKa = 6.23VLRR32 pKa = 11.84VSLVDD37 pKa = 3.87RR38 pKa = 11.84EE39 pKa = 4.3CQKK42 pKa = 10.88VICEE46 pKa = 4.01PLGRR50 pKa = 11.84NYY52 pKa = 9.75RR53 pKa = 11.84VSMPLIAFRR62 pKa = 11.84SGKK65 pKa = 8.95NMKK68 pKa = 9.95HH69 pKa = 6.22LGGAAA74 pKa = 3.13

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

15006

29

1158

220.7

24.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.009 ± 0.635

1.379 ± 0.146

5.378 ± 0.205

6.517 ± 0.306

3.379 ± 0.183

7.597 ± 0.315

2.026 ± 0.178

4.931 ± 0.244

5.131 ± 0.261

8.31 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.619 ± 0.164

4.272 ± 0.222

4.292 ± 0.269

4.212 ± 0.299

6.511 ± 0.287

6.711 ± 0.266

5.824 ± 0.391

6.931 ± 0.232

1.419 ± 0.111

2.552 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski