Chicken stool-associated gemycircularvirus
Average proteome isoelectric point is 8.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L6KW52|A0A1L6KW52_9VIRU Capsid protein OS=Chicken stool-associated gemycircularvirus OX=1930298 PE=4 SV=1
MM1 pKa = 6.97 TRR3 pKa = 11.84 FRR5 pKa = 11.84 LRR7 pKa = 11.84 AKK9 pKa = 10.2 IFFLTYY15 pKa = 9.88 SQIGEE20 pKa = 4.22 DD21 pKa = 3.93 VIQEE25 pKa = 4.06 LRR27 pKa = 11.84 STPTQHH33 pKa = 6.22 FRR35 pKa = 11.84 FIEE38 pKa = 4.3 EE39 pKa = 4.18 VLGTPSDD46 pKa = 3.75 YY47 pKa = 10.65 RR48 pKa = 11.84 LSRR51 pKa = 11.84 EE52 pKa = 3.93 SHH54 pKa = 5.87 QDD56 pKa = 3.22 GASHH60 pKa = 6.11 LHH62 pKa = 6.05 CVVGYY67 pKa = 11.22 AEE69 pKa = 4.23 TQLVNSASLFDD80 pKa = 3.86 YY81 pKa = 10.78 RR82 pKa = 11.84 GAHH85 pKa = 6.31 PNIKK89 pKa = 10.01 SIRR92 pKa = 11.84 GTIKK96 pKa = 10.75 KK97 pKa = 8.29 PWNYY101 pKa = 10.43 AGKK104 pKa = 10.17 DD105 pKa = 3.04 GDD107 pKa = 4.55 IIHH110 pKa = 6.86 EE111 pKa = 4.36 LGGPNGGDD119 pKa = 3.11 SGTGGSIAEE128 pKa = 4.23 RR129 pKa = 11.84 WSHH132 pKa = 7.12 AIDD135 pKa = 4.55 APTEE139 pKa = 3.97 DD140 pKa = 3.72 EE141 pKa = 4.03 FFEE144 pKa = 4.44 RR145 pKa = 11.84 VRR147 pKa = 11.84 MADD150 pKa = 3.08 PRR152 pKa = 11.84 SYY154 pKa = 11.68 VLFNRR159 pKa = 11.84 AIVQYY164 pKa = 11.01 ANKK167 pKa = 9.77 IYY169 pKa = 10.33 RR170 pKa = 11.84 KK171 pKa = 8.14 PRR173 pKa = 11.84 EE174 pKa = 3.89 YY175 pKa = 10.01 TSPRR179 pKa = 11.84 FRR181 pKa = 11.84 LLGGSRR187 pKa = 11.84 IEE189 pKa = 4.24 GWLSQSDD196 pKa = 3.43 LGRR199 pKa = 11.84 RR200 pKa = 11.84 GGEE203 pKa = 3.74 RR204 pKa = 3.19
Molecular weight: 23.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.73
IPC2_protein 8.682
IPC_protein 8.829
Toseland 9.077
ProMoST 9.209
Dawson 9.414
Bjellqvist 9.238
Wikipedia 9.663
Rodwell 9.472
Grimsley 9.516
Solomon 9.531
Lehninger 9.472
Nozaki 9.136
DTASelect 9.209
Thurlkill 9.224
EMBOSS 9.516
Sillero 9.37
Patrickios 4.724
IPC_peptide 9.516
IPC2_peptide 8.024
IPC2.peptide.svr19 7.785
Protein with the highest isoelectric point:
>tr|A0A1L6KW52|A0A1L6KW52_9VIRU Capsid protein OS=Chicken stool-associated gemycircularvirus OX=1930298 PE=4 SV=1
MM1 pKa = 7.84 GYY3 pKa = 10.16 FEE5 pKa = 5.2 EE6 pKa = 4.69 NGYY9 pKa = 10.11 SLSTGLHH16 pKa = 5.85 HH17 pKa = 7.91 SEE19 pKa = 3.51 MAYY22 pKa = 10.37 SRR24 pKa = 11.84 FRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 SFRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 PARR37 pKa = 11.84 TLRR40 pKa = 11.84 RR41 pKa = 11.84 SQHH44 pKa = 4.73 KK45 pKa = 8.15 RR46 pKa = 11.84 TVRR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 FVRR55 pKa = 11.84 RR56 pKa = 11.84 PRR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 MTSRR64 pKa = 11.84 RR65 pKa = 11.84 VRR67 pKa = 11.84 NIASRR72 pKa = 11.84 KK73 pKa = 9.04 KK74 pKa = 10.06 HH75 pKa = 5.38 DD76 pKa = 3.74 TQLGADD82 pKa = 3.93 SNADD86 pKa = 3.42 DD87 pKa = 4.64 SSNPVQLHH95 pKa = 6.38 AGNNYY100 pKa = 8.68 YY101 pKa = 10.68 LWAPSYY107 pKa = 10.85 LEE109 pKa = 3.86 QHH111 pKa = 6.2 EE112 pKa = 4.22 EE113 pKa = 3.96 SYY115 pKa = 11.36 DD116 pKa = 3.7 YY117 pKa = 11.45 VRR119 pKa = 11.84 NNQNVYY125 pKa = 10.06 FRR127 pKa = 11.84 GVQDD131 pKa = 3.94 RR132 pKa = 11.84 VFLSAAFAFTWRR144 pKa = 11.84 RR145 pKa = 11.84 VCFFSYY151 pKa = 10.5 EE152 pKa = 3.98 QFIPALPFFVEE163 pKa = 5.26 APNEE167 pKa = 4.16 DD168 pKa = 4.02 DD169 pKa = 3.73 PQIMRR174 pKa = 11.84 RR175 pKa = 11.84 PLRR178 pKa = 11.84 EE179 pKa = 3.91 ITPKK183 pKa = 9.38 TASEE187 pKa = 4.2 FFSYY191 pKa = 9.82 AWKK194 pKa = 8.9 GTVGVDD200 pKa = 3.83 FSEE203 pKa = 4.13 NTRR206 pKa = 11.84 WNAPLDD212 pKa = 4.29 DD213 pKa = 4.5 KK214 pKa = 11.5 LLTIVYY220 pKa = 9.6 DD221 pKa = 3.84 RR222 pKa = 11.84 SVTINPNYY230 pKa = 9.75 AAPAGATFGKK240 pKa = 10.8 SMTRR244 pKa = 11.84 KK245 pKa = 8.12 MWHH248 pKa = 6.45 PVNKK252 pKa = 9.83 TMMYY256 pKa = 10.12 SDD258 pKa = 4.9 KK259 pKa = 11.03 EE260 pKa = 4.23 NGSTPSSTGWTSMCPNSPGNFYY282 pKa = 10.64 ILDD285 pKa = 3.84 IFSTGQDD292 pKa = 3.42 LPGDD296 pKa = 3.67 LTGVGEE302 pKa = 4.68 FGSEE306 pKa = 3.61 ATVYY310 pKa = 8.37 WHH312 pKa = 6.95 EE313 pKa = 4.1 NN314 pKa = 3.13
Molecular weight: 36.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.801
IPC2_protein 9.428
IPC_protein 9.955
Toseland 9.75
ProMoST 9.692
Dawson 10.087
Bjellqvist 9.867
Wikipedia 10.335
Rodwell 10.175
Grimsley 10.204
Solomon 10.116
Lehninger 10.058
Nozaki 9.78
DTASelect 9.853
Thurlkill 9.897
EMBOSS 10.204
Sillero 9.999
Patrickios 5.054
IPC_peptide 10.101
IPC2_peptide 8.77
IPC2.peptide.svr19 8.38
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
518
204
314
259.0
29.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.178 ± 0.176
0.579 ± 0.053
5.212 ± 0.107
5.792 ± 0.637
5.792 ± 0.529
7.529 ± 1.645
2.896 ± 0.319
4.247 ± 1.556
3.282 ± 0.089
5.792 ± 0.637
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.124 ± 0.68
4.826 ± 1.122
5.212 ± 0.476
3.089 ± 0.204
11.197 ± 0.246
8.687 ± 0.211
5.985 ± 0.644
4.826 ± 0.538
1.931 ± 0.274
4.826 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here