Wenzhou tombus-like virus 10
Average proteome isoelectric point is 7.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KH44|A0A1L3KH44_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 10 OX=1923663 PE=4 SV=1
MM1 pKa = 7.87 TDD3 pKa = 3.12 QQFHH7 pKa = 6.39 MLPKK11 pKa = 10.41 LVRR14 pKa = 11.84 QFVLRR19 pKa = 11.84 RR20 pKa = 11.84 VRR22 pKa = 11.84 TTIRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 CVNRR32 pKa = 11.84 GRR34 pKa = 11.84 GKK36 pKa = 10.81 LLGNKK41 pKa = 8.15 MMHH44 pKa = 6.83 ALNGNTTDD52 pKa = 3.67 YY53 pKa = 11.36 VKK55 pKa = 10.22 WKK57 pKa = 10.46 QSFDD61 pKa = 3.52 EE62 pKa = 4.4 SLKK65 pKa = 7.86 TTNDD69 pKa = 2.76 KK70 pKa = 10.95 SRR72 pKa = 11.84 QQRR75 pKa = 11.84 VDD77 pKa = 3.0 MNIRR81 pKa = 11.84 VSVDD85 pKa = 3.14 MPKK88 pKa = 9.48 TFSLTHH94 pKa = 6.92 DD95 pKa = 4.4 LAAMAFVAVLDD106 pKa = 3.62 GFMYY110 pKa = 10.57 HH111 pKa = 7.01 IAFDD115 pKa = 3.89 YY116 pKa = 10.73 LKK118 pKa = 10.36 KK119 pKa = 10.84 GKK121 pKa = 9.79 AKK123 pKa = 10.37 RR124 pKa = 11.84 IIVEE128 pKa = 4.05 MPHH131 pKa = 6.51 PVNGCTKK138 pKa = 10.02 QCGWDD143 pKa = 3.69 DD144 pKa = 3.77 NVVRR148 pKa = 11.84 SYY150 pKa = 10.26 PAPLLINDD158 pKa = 5.51 IIPIPLPCEE167 pKa = 3.84 VTFGRR172 pKa = 11.84 NGLQKK177 pKa = 10.73 HH178 pKa = 6.57 FITDD182 pKa = 3.09 DD183 pKa = 3.81 DD184 pKa = 4.39 YY185 pKa = 12.13 ADD187 pKa = 4.09 FDD189 pKa = 4.35 IPLDD193 pKa = 4.13 DD194 pKa = 4.53 SLSNLQYY201 pKa = 11.44 DD202 pKa = 4.11 SDD204 pKa = 4.09 TKK206 pKa = 10.67 SWNSAGSPAQSTSSNVSGVTAPSSSTPTPSTSAPPPKK243 pKa = 10.18 SNKK246 pKa = 8.52 QLSIVRR252 pKa = 11.84 RR253 pKa = 11.84 SDD255 pKa = 3.43 SKK257 pKa = 10.55 VQTPSVEE264 pKa = 3.75 QRR266 pKa = 11.84 VCVVPPLQPTPSQPTPPPTLAAPRR290 pKa = 11.84 VVSEE294 pKa = 5.35 GYY296 pKa = 8.56 TEE298 pKa = 3.92 PHH300 pKa = 5.67 TRR302 pKa = 11.84 LHH304 pKa = 5.95 FLARR308 pKa = 11.84 HH309 pKa = 5.97 AAQISEE315 pKa = 4.44 VEE317 pKa = 4.45 RR318 pKa = 11.84 PQLPDD323 pKa = 3.26 FTEE326 pKa = 3.77 NPYY329 pKa = 10.44 RR330 pKa = 11.84 KK331 pKa = 8.73 WVAEE335 pKa = 3.92 VLLGDD340 pKa = 3.86 RR341 pKa = 11.84 NNPNMQFPLLDD352 pKa = 3.75 LRR354 pKa = 11.84 SPLAIWMSSGSFKK367 pKa = 10.71 TVMRR371 pKa = 11.84 KK372 pKa = 9.85 HH373 pKa = 5.27 IQYY376 pKa = 9.53 GNSMGLDD383 pKa = 3.27 AEE385 pKa = 4.47 MLVHH389 pKa = 6.89 LVRR392 pKa = 11.84 LITEE396 pKa = 3.86 KK397 pKa = 10.95 RR398 pKa = 11.84 EE399 pKa = 3.97 ASSFATLYY407 pKa = 10.47 HH408 pKa = 7.08 KK409 pKa = 10.72 AYY411 pKa = 8.76 TWASHH416 pKa = 6.21 NRR418 pKa = 11.84 EE419 pKa = 4.22 DD420 pKa = 3.98 WPLGLSDD427 pKa = 4.34 AQSAGCARR435 pKa = 11.84 YY436 pKa = 7.7 TLGTSSLYY444 pKa = 10.9 ASLLNNITPGVSEE457 pKa = 4.49 EE458 pKa = 4.42 SFDD461 pKa = 4.45 VDD463 pKa = 3.53 PQINPVYY470 pKa = 9.25 ATKK473 pKa = 10.5 NRR475 pKa = 11.84 RR476 pKa = 11.84 NFVYY480 pKa = 10.47 NVVLIILFILFSYY493 pKa = 9.97 AAYY496 pKa = 8.68 HH497 pKa = 5.66 TFRR500 pKa = 11.84 FARR503 pKa = 11.84 YY504 pKa = 9.53 VDD506 pKa = 2.94 GHH508 pKa = 5.56 YY509 pKa = 11.2 NRR511 pKa = 11.84 FAAYY515 pKa = 10.24 ADD517 pKa = 3.42 NSFEE521 pKa = 4.25 NFGNQFTAFAAVTYY535 pKa = 10.21 GSSCFTHH542 pKa = 6.42 EE543 pKa = 5.45 RR544 pKa = 11.84 IGCALGDD551 pKa = 3.57 NDD553 pKa = 4.96 CYY555 pKa = 10.97 RR556 pKa = 11.84 RR557 pKa = 11.84 NCRR560 pKa = 11.84 DD561 pKa = 3.22 LLRR564 pKa = 11.84 IYY566 pKa = 10.75 HH567 pKa = 6.92 PDD569 pKa = 2.84 KK570 pKa = 11.09 GGNVDD575 pKa = 3.73 SYY577 pKa = 9.07 THH579 pKa = 5.7 VARR582 pKa = 11.84 DD583 pKa = 3.82 CEE585 pKa = 4.19 FLNKK589 pKa = 10.31 GGEE592 pKa = 4.03 QPVYY596 pKa = 10.42 EE597 pKa = 4.22 NCAYY601 pKa = 10.4 ANKK604 pKa = 10.23 EE605 pKa = 3.92 EE606 pKa = 4.4 GVSFEE611 pKa = 4.8 LNEE614 pKa = 4.11 LYY616 pKa = 11.06 VRR618 pKa = 11.84 FSQFAKK624 pKa = 10.12 TRR626 pKa = 11.84 GIDD629 pKa = 3.56 ADD631 pKa = 4.07 FGDD634 pKa = 3.94 YY635 pKa = 10.8 DD636 pKa = 4.48 YY637 pKa = 11.2 FFQAAPSSNPICDD650 pKa = 3.05 AVKK653 pKa = 10.66 NGEE656 pKa = 4.34 SVPKK660 pKa = 8.98 EE661 pKa = 3.77 VLMICQFFKK670 pKa = 11.13 LRR672 pKa = 11.84 DD673 pKa = 3.96 FRR675 pKa = 11.84 PSCYY679 pKa = 10.04 NYY681 pKa = 10.69 SSFFNAYY688 pKa = 9.56 GSMYY692 pKa = 10.06 HH693 pKa = 6.31 NGIIFQSIFPTMVSICVSFGKK714 pKa = 10.02 GQTMRR719 pKa = 11.84 EE720 pKa = 3.88 HH721 pKa = 6.67 MFTEE725 pKa = 5.19 VYY727 pKa = 9.17 YY728 pKa = 10.83 TMAFQYY734 pKa = 10.88 CCVCCFVALISVLFATLAIYY754 pKa = 7.49 PTVKK758 pKa = 10.16 KK759 pKa = 10.85 AFIEE763 pKa = 3.87 RR764 pKa = 11.84 GFRR767 pKa = 11.84 VGCSVWVSKK776 pKa = 11.02 CSVTGVTSAAIMATFILIKK795 pKa = 10.47 EE796 pKa = 4.04 RR797 pKa = 11.84 DD798 pKa = 3.93 LLCRR802 pKa = 11.84 ASSAAYY808 pKa = 9.23 VARR811 pKa = 11.84 ALKK814 pKa = 10.27 EE815 pKa = 3.78 LLLLVISGLRR825 pKa = 11.84 SLWAAAAAA833 pKa = 4.07
Molecular weight: 93.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.513
IPC2_protein 7.761
IPC_protein 7.527
Toseland 7.117
ProMoST 8.156
Dawson 8.244
Bjellqvist 8.682
Wikipedia 8.17
Rodwell 8.273
Grimsley 7.161
Solomon 8.287
Lehninger 8.302
Nozaki 8.858
DTASelect 8.375
Thurlkill 8.419
EMBOSS 8.448
Sillero 8.756
Patrickios 3.973
IPC_peptide 8.273
IPC2_peptide 7.761
IPC2.peptide.svr19 7.839
Protein with the highest isoelectric point:
>tr|A0A1L3KH44|A0A1L3KH44_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 10 OX=1923663 PE=4 SV=1
MM1 pKa = 7.17 FGYY4 pKa = 9.99 RR5 pKa = 11.84 RR6 pKa = 11.84 HH7 pKa = 6.09 ICSHH11 pKa = 6.6 HH12 pKa = 6.69 GNLHH16 pKa = 5.8 TYY18 pKa = 7.89 QRR20 pKa = 11.84 EE21 pKa = 3.91 RR22 pKa = 11.84 SIVPCVVSSVCSSSSEE38 pKa = 3.9 GAVIVGNKK46 pKa = 7.3 WVAQPLGSGCSMSRR60 pKa = 11.84 TNQQSEE66 pKa = 4.36 SCSAKK71 pKa = 9.11 FCKK74 pKa = 10.15 FLTIKK79 pKa = 10.59 NSRR82 pKa = 11.84 TWDD85 pKa = 4.28 PIVFSPCSHH94 pKa = 6.33 NFMLAAKK101 pKa = 10.09 SRR103 pKa = 11.84 ILKK106 pKa = 7.52 EE107 pKa = 4.22 TPQPEE112 pKa = 4.09 DD113 pKa = 3.29 EE114 pKa = 4.31 YY115 pKa = 11.47 LRR117 pKa = 11.84 RR118 pKa = 11.84 FALSLHH124 pKa = 6.17 AVAKK128 pKa = 10.47 SIFVKK133 pKa = 8.71 QTPLTRR139 pKa = 11.84 EE140 pKa = 4.1 EE141 pKa = 4.11 FLSAYY146 pKa = 10.15 SGSKK150 pKa = 9.11 RR151 pKa = 11.84 LRR153 pKa = 11.84 YY154 pKa = 10.25 AMAADD159 pKa = 3.66 RR160 pKa = 11.84 VYY162 pKa = 11.21 DD163 pKa = 3.77 VDD165 pKa = 4.95 DD166 pKa = 4.75 GSIKK170 pKa = 10.21 HH171 pKa = 6.1 ASSFVKK177 pKa = 10.43 LQRR180 pKa = 11.84 FDD182 pKa = 3.64 PTSKK186 pKa = 8.93 EE187 pKa = 3.98 HH188 pKa = 4.85 QVPRR192 pKa = 11.84 VVSFRR197 pKa = 11.84 QYY199 pKa = 10.59 TNAFSLGRR207 pKa = 11.84 YY208 pKa = 8.38 IRR210 pKa = 11.84 PLEE213 pKa = 4.12 HH214 pKa = 6.42 QFVNAPSFGRR224 pKa = 11.84 FKK226 pKa = 10.82 GYY228 pKa = 10.0 RR229 pKa = 11.84 WLAKK233 pKa = 9.13 TRR235 pKa = 11.84 NLTQRR240 pKa = 11.84 ATDD243 pKa = 3.86 ISLIWDD249 pKa = 4.44 MIPNVCCVSIDD260 pKa = 3.35 ASRR263 pKa = 11.84 FEE265 pKa = 4.05 AHH267 pKa = 6.03 VSKK270 pKa = 11.29 DD271 pKa = 3.35 MLSLEE276 pKa = 4.17 FEE278 pKa = 4.71 TYY280 pKa = 10.39 SDD282 pKa = 4.21 AYY284 pKa = 10.69 RR285 pKa = 11.84 GDD287 pKa = 3.34 ARR289 pKa = 11.84 LVKK292 pKa = 9.6 MLKK295 pKa = 10.69 AMLATKK301 pKa = 8.92 MHH303 pKa = 6.3 TKK305 pKa = 10.63 YY306 pKa = 10.78 GLKK309 pKa = 10.32 CSILGRR315 pKa = 11.84 RR316 pKa = 11.84 LSGDD320 pKa = 2.89 MCTSLGNALLMCGMLNFISEE340 pKa = 4.56 DD341 pKa = 3.46 LNIDD345 pKa = 3.82 FKK347 pKa = 11.18 IYY349 pKa = 10.53 DD350 pKa = 4.7 DD351 pKa = 5.94 GDD353 pKa = 3.91 DD354 pKa = 4.88 CLLFMNKK361 pKa = 9.36 NLLPRR366 pKa = 11.84 LEE368 pKa = 4.01 EE369 pKa = 3.6 MRR371 pKa = 11.84 NCFKK375 pKa = 11.04 RR376 pKa = 11.84 LGFDD380 pKa = 3.27 VRR382 pKa = 11.84 VDD384 pKa = 4.05 KK385 pKa = 10.83 IAYY388 pKa = 8.35 CLEE391 pKa = 4.44 EE392 pKa = 4.24 IVFCQSHH399 pKa = 7.07 PIYY402 pKa = 9.29 WAPGQGVLCPDD413 pKa = 4.17 PDD415 pKa = 3.41 KK416 pKa = 11.52 HH417 pKa = 6.2 MSALYY422 pKa = 9.79 TGTSYY427 pKa = 11.14 GRR429 pKa = 11.84 SARR432 pKa = 11.84 RR433 pKa = 11.84 DD434 pKa = 3.34 RR435 pKa = 11.84 LIAFSTAMCCLSFLTNIPILGAFHH459 pKa = 7.05 SMVHH463 pKa = 6.57 RR464 pKa = 11.84 ATATKK469 pKa = 10.42 RR470 pKa = 11.84 KK471 pKa = 9.06 MIFPDD476 pKa = 3.07 RR477 pKa = 11.84 TLEE480 pKa = 3.78 YY481 pKa = 10.12 LYY483 pKa = 10.8 KK484 pKa = 10.95 NNSSCSKK491 pKa = 10.81 CPGLNDD497 pKa = 3.62 ALRR500 pKa = 11.84 VSFYY504 pKa = 11.02 LAFGYY509 pKa = 8.71 TPSQQEE515 pKa = 3.66 QLEE518 pKa = 4.34 EE519 pKa = 4.38 HH520 pKa = 6.93 FDD522 pKa = 3.45 TTLLHH527 pKa = 6.32 FKK529 pKa = 10.31 HH530 pKa = 6.0 YY531 pKa = 11.07 AEE533 pKa = 5.03 LSQPVFDD540 pKa = 4.94 YY541 pKa = 9.87 ITWTSVTDD549 pKa = 3.94 TPACQLDD556 pKa = 3.72 APTT559 pKa = 4.45
Molecular weight: 63.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.851
IPC2_protein 7.995
IPC_protein 7.761
Toseland 7.337
ProMoST 8.375
Dawson 8.507
Bjellqvist 8.916
Wikipedia 8.419
Rodwell 8.536
Grimsley 7.366
Solomon 8.551
Lehninger 8.565
Nozaki 9.136
DTASelect 8.609
Thurlkill 8.653
EMBOSS 8.697
Sillero 8.99
Patrickios 4.126
IPC_peptide 8.536
IPC2_peptide 8.024
IPC2.peptide.svr19 8.139
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1392
559
833
696.0
78.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.543 ± 0.44
3.233 ± 0.415
5.46 ± 0.051
3.951 ± 0.096
5.819 ± 0.056
4.885 ± 0.138
2.73 ± 0.289
4.454 ± 0.011
4.813 ± 0.327
8.477 ± 0.593
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.802 ± 0.247
4.382 ± 0.581
5.029 ± 0.434
3.376 ± 0.092
6.537 ± 0.26
8.908 ± 0.444
5.675 ± 0.077
6.466 ± 0.755
1.078 ± 0.003
4.382 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here