Niminivirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; unclassified Geminiviridae

Average proteome isoelectric point is 8.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7LLL3|J7LLL3_9GEMI Replication-associated protein OS=Niminivirus OX=1229325 PE=3 SV=2
MM1 pKa = 7.61QYY3 pKa = 10.93PNMPFRR9 pKa = 11.84IQGRR13 pKa = 11.84HH14 pKa = 5.61LFLTYY19 pKa = 8.88PQCHH23 pKa = 6.3LTHH26 pKa = 6.18EE27 pKa = 5.15AIYY30 pKa = 10.79NHH32 pKa = 7.29LIDD35 pKa = 5.48LPVGNEE41 pKa = 3.84KK42 pKa = 8.85PTKK45 pKa = 9.73IVVAKK50 pKa = 9.61EE51 pKa = 3.73LHH53 pKa = 6.52EE54 pKa = 5.35DD55 pKa = 3.71GHH57 pKa = 5.91PHH59 pKa = 5.29FHH61 pKa = 7.77AYY63 pKa = 10.46LCFDD67 pKa = 3.22KK68 pKa = 11.0RR69 pKa = 11.84RR70 pKa = 11.84DD71 pKa = 3.43LRR73 pKa = 11.84YY74 pKa = 9.45QRR76 pKa = 11.84HH77 pKa = 5.36FDD79 pKa = 2.97ISGYY83 pKa = 9.98HH84 pKa = 6.59PNIQVCRR91 pKa = 11.84SVKK94 pKa = 10.44DD95 pKa = 3.3VLKK98 pKa = 10.94YY99 pKa = 7.9VTKK102 pKa = 10.71DD103 pKa = 3.15GEE105 pKa = 4.39YY106 pKa = 9.17MSNFTVIKK114 pKa = 8.46PTIHH118 pKa = 6.1QLIEE122 pKa = 3.98RR123 pKa = 11.84ADD125 pKa = 4.1DD126 pKa = 3.6EE127 pKa = 4.9TSFALSALEE136 pKa = 5.2HH137 pKa = 6.16YY138 pKa = 9.82DD139 pKa = 4.37KK140 pKa = 11.69NFANCFNSWMSLYY153 pKa = 11.13KK154 pKa = 9.74MLKK157 pKa = 9.62SKK159 pKa = 10.84KK160 pKa = 9.39KK161 pKa = 9.39VCDD164 pKa = 3.79PVSDD168 pKa = 3.66WTLMKK173 pKa = 9.97MDD175 pKa = 5.56DD176 pKa = 3.73LHH178 pKa = 8.26LLARR182 pKa = 11.84ILSITAHH189 pKa = 5.84QKK191 pKa = 10.41DD192 pKa = 3.62GSRR195 pKa = 11.84TKK197 pKa = 10.66SIWLFGPSKK206 pKa = 9.69TGKK209 pKa = 8.34STLARR214 pKa = 11.84SIGKK218 pKa = 8.19HH219 pKa = 6.03AYY221 pKa = 7.54MQNTWCVDD229 pKa = 3.54EE230 pKa = 5.79LSDD233 pKa = 4.36DD234 pKa = 3.71AQYY237 pKa = 11.73LVLDD241 pKa = 5.28DD242 pKa = 4.93IAWDD246 pKa = 3.52TWKK249 pKa = 9.36WQYY252 pKa = 11.53KK253 pKa = 9.89SVLGCQKK260 pKa = 10.94DD261 pKa = 3.76VIFTGKK267 pKa = 9.04YY268 pKa = 8.43ARR270 pKa = 11.84PRR272 pKa = 11.84RR273 pKa = 11.84FNFNMPCIVCSNEE286 pKa = 3.74KK287 pKa = 10.2PVFSVDD293 pKa = 3.05EE294 pKa = 4.46LNWLDD299 pKa = 3.57VNIDD303 pKa = 3.52FFEE306 pKa = 4.77INNKK310 pKa = 9.95LYY312 pKa = 10.99

Molecular weight: 36.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7LJE9|J7LJE9_9GEMI Putative movement protein OS=Niminivirus OX=1229325 PE=4 SV=2
MM1 pKa = 7.44KK2 pKa = 10.07RR3 pKa = 11.84PSFFSVAKK11 pKa = 10.56AGFKK15 pKa = 10.4RR16 pKa = 11.84YY17 pKa = 10.04GPTIARR23 pKa = 11.84KK24 pKa = 9.2VGKK27 pKa = 9.22HH28 pKa = 4.5ALKK31 pKa = 10.44YY32 pKa = 10.24GYY34 pKa = 10.14RR35 pKa = 11.84KK36 pKa = 8.46LTQSGKK42 pKa = 7.16STRR45 pKa = 11.84EE46 pKa = 4.03TAPLTYY52 pKa = 10.94DD53 pKa = 3.27NDD55 pKa = 3.33FKK57 pKa = 11.06TDD59 pKa = 3.34YY60 pKa = 9.97RR61 pKa = 11.84YY62 pKa = 11.02KK63 pKa = 10.74RR64 pKa = 11.84MPKK67 pKa = 9.22RR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84WKK74 pKa = 10.39RR75 pKa = 11.84FTKK78 pKa = 10.23KK79 pKa = 9.87VNAVINRR86 pKa = 11.84SQGLKK91 pKa = 9.86KK92 pKa = 10.47VLYY95 pKa = 9.87SDD97 pKa = 4.03IVRR100 pKa = 11.84LSSNVSQCAYY110 pKa = 9.74HH111 pKa = 6.81SAMLYY116 pKa = 9.77TPDD119 pKa = 4.71ARR121 pKa = 11.84VTDD124 pKa = 4.53LSADD128 pKa = 3.16LGMIFRR134 pKa = 11.84DD135 pKa = 3.14ILGAVAYY142 pKa = 10.78NDD144 pKa = 3.3IQNILVQGDD153 pKa = 3.46VDD155 pKa = 3.66KK156 pKa = 11.19KK157 pKa = 10.95IRR159 pKa = 11.84FEE161 pKa = 4.12SANMDD166 pKa = 3.53VSWRR170 pKa = 11.84NVGANPVIIDD180 pKa = 4.15LYY182 pKa = 9.79YY183 pKa = 9.54VRR185 pKa = 11.84CRR187 pKa = 11.84KK188 pKa = 8.09TFGLTGADD196 pKa = 3.86ADD198 pKa = 4.58NNTQGIFSLGFVKK211 pKa = 10.51QGVIVDD217 pKa = 4.11EE218 pKa = 4.48EE219 pKa = 4.55DD220 pKa = 3.75GNTVGSNRR228 pKa = 11.84QFALTVGSTPFQSSLFCQTYY248 pKa = 10.47RR249 pKa = 11.84ILSKK253 pKa = 10.73KK254 pKa = 10.1RR255 pKa = 11.84ITIAPGNTVSMTLKK269 pKa = 10.49DD270 pKa = 3.68SRR272 pKa = 11.84NKK274 pKa = 9.6VVNAMDD280 pKa = 3.35TRR282 pKa = 11.84ARR284 pKa = 11.84ICMRR288 pKa = 11.84NLTHH292 pKa = 7.35GYY294 pKa = 9.98LFQLYY299 pKa = 9.0GVPGLNGDD307 pKa = 4.01VPVTALASDD316 pKa = 3.98VVFSVQKK323 pKa = 10.47RR324 pKa = 11.84YY325 pKa = 10.18GFYY328 pKa = 10.9LPVSGKK334 pKa = 9.96DD335 pKa = 3.15QTSNIPLL342 pKa = 3.76

Molecular weight: 38.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

821

78

342

205.3

23.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.725 ± 0.573

1.949 ± 0.504

6.456 ± 0.825

2.436 ± 0.91

5.725 ± 1.696

5.116 ± 1.169

2.801 ± 1.099

5.359 ± 0.257

7.065 ± 0.872

9.013 ± 1.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.261

4.872 ± 0.484

4.263 ± 0.494

3.41 ± 0.32

7.186 ± 1.314

8.283 ± 1.467

5.847 ± 0.86

6.456 ± 1.319

1.218 ± 0.605

4.263 ± 0.675
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski