Acidipila sp. EB88

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Acidipila; unclassified Acidipila

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3402 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P0XA44|A0A3P0XA44_9BACT Glycosyltransferase OS=Acidipila sp. EB88 OX=2305226 GN=D1Y84_14980 PE=4 SV=1
MM1 pKa = 7.51TSQEE5 pKa = 3.86QAMLNDD11 pKa = 4.34LVHH14 pKa = 7.07KK15 pKa = 10.55INSTNLEE22 pKa = 4.15EE23 pKa = 5.56KK24 pKa = 10.68DD25 pKa = 3.69DD26 pKa = 4.17AAEE29 pKa = 3.77QLLRR33 pKa = 11.84DD34 pKa = 3.81GLGRR38 pKa = 11.84NQDD41 pKa = 3.87ALYY44 pKa = 10.04MLAQTVLVQNIALEE58 pKa = 4.1QAKK61 pKa = 9.1QQLMQMQQAPAAPAKK76 pKa = 9.19ATSFLGSLLGHH87 pKa = 7.36RR88 pKa = 11.84DD89 pKa = 3.98PQPQAPPPQQQGGYY103 pKa = 7.3QQVQYY108 pKa = 10.55GQPQYY113 pKa = 11.21AQPQYY118 pKa = 10.71AQPQGGFAPPPSGQPSFLRR137 pKa = 11.84SAATTAAGVAAGALAFQGVEE157 pKa = 4.21SLLHH161 pKa = 6.41GGMGGGGGFGGEE173 pKa = 4.36GFGGGGFGGGEE184 pKa = 3.96APRR187 pKa = 11.84EE188 pKa = 3.86EE189 pKa = 5.05IINNYY194 pKa = 8.89YY195 pKa = 10.61DD196 pKa = 3.66SPGGGSGGGNYY207 pKa = 10.08NSGPDD212 pKa = 3.92EE213 pKa = 4.16QPQGPFDD220 pKa = 4.03SGSNDD225 pKa = 2.74ASYY228 pKa = 11.73GDD230 pKa = 5.4DD231 pKa = 3.71YY232 pKa = 11.61TDD234 pKa = 3.45GGGDD238 pKa = 3.66FGGDD242 pKa = 3.37DD243 pKa = 4.11DD244 pKa = 5.5TNFSS248 pKa = 3.71

Molecular weight: 25.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P0X897|A0A3P0X897_9BACT Energy transducer TonB OS=Acidipila sp. EB88 OX=2305226 GN=D1Y84_08775 PE=3 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.02RR4 pKa = 11.84TFQPNRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.69RR13 pKa = 11.84AKK15 pKa = 9.37THH17 pKa = 5.09GFRR20 pKa = 11.84TRR22 pKa = 11.84MKK24 pKa = 8.84TNSGAAVLNRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.03GRR40 pKa = 11.84HH41 pKa = 5.72KK42 pKa = 10.29IAVSAGFRR50 pKa = 11.84DD51 pKa = 3.66

Molecular weight: 5.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3402

0

3402

1244068

23

2741

365.7

39.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.185 ± 0.052

0.885 ± 0.014

4.92 ± 0.03

5.318 ± 0.042

3.709 ± 0.028

8.166 ± 0.044

2.453 ± 0.022

4.301 ± 0.028

2.988 ± 0.027

10.369 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.089 ± 0.021

3.033 ± 0.044

5.588 ± 0.031

4.087 ± 0.027

6.458 ± 0.049

6.188 ± 0.038

6.048 ± 0.046

7.204 ± 0.036

1.377 ± 0.02

2.635 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski