Streptococcus satellite phage Javan89

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A1K4|A0A4D6A1K4_9VIRU ArpU family encoded transcriptional regulator OS=Streptococcus satellite phage Javan89 OX=2558866 GN=JavanS89_0003 PE=4 SV=1
MM1 pKa = 6.67MQEE4 pKa = 4.55LNLTPTQTLILFIVLGLVGLLLSLSKK30 pKa = 10.47PLIEE34 pKa = 5.57IDD36 pKa = 4.32LPEE39 pKa = 4.55DD40 pKa = 3.66TQVPKK45 pKa = 10.6PPQNANYY52 pKa = 9.61GAYY55 pKa = 9.23IQSQNHH61 pKa = 5.87YY62 pKa = 9.82YY63 pKa = 10.4NN64 pKa = 3.73

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZXF3|A0A4D5ZXF3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan89 OX=2558866 GN=JavanS89_0001 PE=4 SV=1
MM1 pKa = 7.61NIKK4 pKa = 9.82EE5 pKa = 4.16YY6 pKa = 10.51KK7 pKa = 9.44KK8 pKa = 10.5KK9 pKa = 10.71NGTIVYY15 pKa = 7.42RR16 pKa = 11.84TSVYY20 pKa = 10.7LGVDD24 pKa = 2.96QVTGKK29 pKa = 10.41KK30 pKa = 10.32ARR32 pKa = 11.84TTITASTKK40 pKa = 10.32KK41 pKa = 10.14GVKK44 pKa = 9.43IKK46 pKa = 10.92ARR48 pKa = 11.84DD49 pKa = 3.59ALNNFAMNGYY59 pKa = 6.25TVKK62 pKa = 10.52EE63 pKa = 4.27KK64 pKa = 9.63PTVTTYY70 pKa = 11.25KK71 pKa = 10.56EE72 pKa = 4.08LTALWWEE79 pKa = 4.56SYY81 pKa = 11.25KK82 pKa = 10.03NTIKK86 pKa = 10.76PNSRR90 pKa = 11.84QSMEE94 pKa = 4.4GIVRR98 pKa = 11.84LHH100 pKa = 6.69ILPAFGDD107 pKa = 4.16CKK109 pKa = 10.5LSRR112 pKa = 11.84LTTPVIQQQVNKK124 pKa = 8.75WANNANKK131 pKa = 10.06GIKK134 pKa = 9.07GAYY137 pKa = 9.42ANYY140 pKa = 10.79SFLNNINRR148 pKa = 11.84RR149 pKa = 11.84ILQYY153 pKa = 10.72GVTMQVIEE161 pKa = 4.63HH162 pKa = 6.18NPARR166 pKa = 11.84DD167 pKa = 3.48VIIPRR172 pKa = 11.84KK173 pKa = 9.5QNNKK177 pKa = 5.23EE178 pKa = 3.95HH179 pKa = 6.3KK180 pKa = 10.5VKK182 pKa = 10.63FFSNN186 pKa = 3.41

Molecular weight: 21.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

3494

48

534

129.4

15.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.581 ± 0.651

0.515 ± 0.132

6.153 ± 0.54

8.615 ± 0.664

4.408 ± 0.411

4.408 ± 0.555

1.631 ± 0.192

7.041 ± 0.461

9.273 ± 0.381

9.502 ± 0.547

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.576 ± 0.18

5.524 ± 0.468

2.318 ± 0.204

4.522 ± 0.266

4.694 ± 0.288

6.096 ± 0.341

5.781 ± 0.581

5.667 ± 0.385

0.973 ± 0.146

4.722 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski