Vibrio phage MZH0603

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A650EQQ2|A0A650EQQ2_9CAUD Uncharacterized protein OS=Vibrio phage MZH0603 OX=2664193 PE=4 SV=1
MM1 pKa = 7.12NACEE5 pKa = 4.57PGLQRR10 pKa = 11.84FIKK13 pKa = 8.57QTNNTEE19 pKa = 4.06EE20 pKa = 4.13PVEE23 pKa = 4.12VSSLIGGEE31 pKa = 4.09NYY33 pKa = 10.65YY34 pKa = 11.22SDD36 pKa = 4.54FLWLAEE42 pKa = 3.9GTLPKK47 pKa = 10.43EE48 pKa = 3.99RR49 pKa = 11.84IVRR52 pKa = 11.84FACDD56 pKa = 2.89VALINIEE63 pKa = 4.2LIKK66 pKa = 10.45PYY68 pKa = 10.17TEE70 pKa = 3.92QFDD73 pKa = 5.0LIVGFLNNPDD83 pKa = 4.12ANTDD87 pKa = 3.52AVVDD91 pKa = 4.12AVDD94 pKa = 3.76AAVDD98 pKa = 3.63AADD101 pKa = 4.44ADD103 pKa = 4.09ADD105 pKa = 3.91ATDD108 pKa = 3.48VNAAYY113 pKa = 9.9AAAYY117 pKa = 8.55AAYY120 pKa = 9.85AAACAAATDD129 pKa = 4.1DD130 pKa = 4.29TATDD134 pKa = 3.77DD135 pKa = 4.48AATAVANTADD145 pKa = 3.78AADD148 pKa = 3.99SEE150 pKa = 4.8EE151 pKa = 5.5KK152 pKa = 10.51VDD154 pKa = 3.69QLLIEE159 pKa = 4.4MFNN162 pKa = 3.38

Molecular weight: 17.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A650EQQ0|A0A650EQQ0_9CAUD Uncharacterized protein OS=Vibrio phage MZH0603 OX=2664193 PE=4 SV=1
MM1 pKa = 7.63CAGPLKK7 pKa = 10.14PKK9 pKa = 10.55APAAVAPPPPPPKK22 pKa = 10.51APEE25 pKa = 3.79AAVAPEE31 pKa = 4.64APTQAGGAGSQQGDD45 pKa = 3.24QARR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84AQGQSTRR58 pKa = 11.84STILTGSRR66 pKa = 11.84GVQDD70 pKa = 3.8GAGTATKK77 pKa = 10.6SLLGQQ82 pKa = 3.66

Molecular weight: 8.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13436

45

1902

248.8

27.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.299 ± 0.536

1.191 ± 0.213

6.721 ± 0.312

6.959 ± 0.382

3.788 ± 0.181

6.832 ± 0.222

2.158 ± 0.219

5.582 ± 0.23

6.036 ± 0.321

7.807 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.806 ± 0.248

4.652 ± 0.215

3.721 ± 0.355

4.168 ± 0.313

5.001 ± 0.281

6.14 ± 0.284

6.289 ± 0.388

6.914 ± 0.294

1.541 ± 0.125

3.394 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski