Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Debaryomycetaceae; Debaryomyces; Debaryomyces hansenii

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6284 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6BNJ7|Q6BNJ7_DEBHA DEHA2E21142p OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=DEHA2E21142g PE=4 SV=2
MM1 pKa = 7.7TEE3 pKa = 3.92PCPAVKK9 pKa = 9.02LTEE12 pKa = 4.16STPKK16 pKa = 10.69NEE18 pKa = 3.77AEE20 pKa = 4.44EE21 pKa = 5.21YY22 pKa = 10.15FDD24 pKa = 5.12QYY26 pKa = 11.76QKK28 pKa = 9.94TKK30 pKa = 9.95ADD32 pKa = 3.33SSATVGNEE40 pKa = 3.79DD41 pKa = 3.39GTISQEE47 pKa = 4.02PKK49 pKa = 9.49ATQDD53 pKa = 3.25MQLDD57 pKa = 4.15VPQKK61 pKa = 10.64KK62 pKa = 9.21EE63 pKa = 3.88RR64 pKa = 11.84NNNSDD69 pKa = 5.55DD70 pKa = 5.89DD71 pKa = 6.16DD72 pKa = 7.42DD73 pKa = 7.48DD74 pKa = 7.31DD75 pKa = 6.85DD76 pKa = 5.94DD77 pKa = 6.32SSDD80 pKa = 3.6VTSATISEE88 pKa = 4.15QGSGYY93 pKa = 9.86EE94 pKa = 4.15RR95 pKa = 11.84NSNEE99 pKa = 4.04CTEE102 pKa = 4.24CAIDD106 pKa = 5.45LIGCFGLFDD115 pKa = 4.07SCCPSTGEE123 pKa = 4.8GFITNLGVFCGNIIVGCCKK142 pKa = 10.42CC143 pKa = 3.98

Molecular weight: 15.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6BIK5|Q6BIK5_DEBHA DEHA2G09658p OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=DEHA2G09658g PE=4 SV=2
MM1 pKa = 7.94PSQKK5 pKa = 10.41SFRR8 pKa = 11.84TKK10 pKa = 10.38QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 9.21QNRR21 pKa = 11.84PLPQWIRR28 pKa = 11.84LRR30 pKa = 11.84SGNTIRR36 pKa = 11.84YY37 pKa = 5.78NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.11WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 11.12LNII51 pKa = 3.83

Molecular weight: 6.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6284

0

6284

3008975

30

4999

478.8

54.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.16 ± 0.029

1.165 ± 0.01

6.172 ± 0.022

6.569 ± 0.03

4.47 ± 0.021

5.2 ± 0.034

2.087 ± 0.011

7.046 ± 0.025

7.376 ± 0.03

9.221 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.014 ± 0.01

6.658 ± 0.03

4.244 ± 0.026

3.812 ± 0.024

4.011 ± 0.017

9.302 ± 0.044

5.475 ± 0.023

5.423 ± 0.022

0.991 ± 0.008

3.602 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski