Exophiala sideris

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae; Exophiala

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10841 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D1W667|A0A0D1W667_9EURO Methyltransf_11 domain-containing protein OS=Exophiala sideris OX=1016849 GN=PV11_06125 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.21SVSSLSITLLLGLATGSVGLPATTEE27 pKa = 4.56DD28 pKa = 4.17GDD30 pKa = 3.95SHH32 pKa = 6.74GWYY35 pKa = 9.78DD36 pKa = 4.01WNPSTAVTSTSTSFSLSVPSSLPGFAGAPASGFGNTNSNGFAPSVSIKK84 pKa = 9.28TVEE87 pKa = 4.07VTKK90 pKa = 10.86TEE92 pKa = 4.16AVCAATEE99 pKa = 4.01EE100 pKa = 4.79SVVGVGTSTGGSDD113 pKa = 3.47SSHH116 pKa = 6.56SGSGSDD122 pKa = 3.34SSSGSGSGSGSGFVSSSGGSWSEE145 pKa = 4.1SGSTYY150 pKa = 10.31YY151 pKa = 10.95AQAPGANSSLSSPTYY166 pKa = 8.86QPYY169 pKa = 9.86VAGPTLLPAVHH180 pKa = 7.14WDD182 pKa = 3.62YY183 pKa = 11.35PVDD186 pKa = 4.73DD187 pKa = 4.79IRR189 pKa = 11.84NLGPINSSDD198 pKa = 4.4LYY200 pKa = 9.15YY201 pKa = 10.38TSNGIADD208 pKa = 4.15PGVQHH213 pKa = 6.38VFASLSTTLQYY224 pKa = 11.31DD225 pKa = 3.91AVVLDD230 pKa = 3.68HH231 pKa = 7.07SSYY234 pKa = 8.97VTSVSCSSTGILVAFEE250 pKa = 4.39SIEE253 pKa = 3.99AFRR256 pKa = 11.84YY257 pKa = 10.36ACDD260 pKa = 2.75SWSAVEE266 pKa = 4.4EE267 pKa = 4.44FVLVTYY273 pKa = 10.52TDD275 pKa = 3.43GCGASDD281 pKa = 4.01DD282 pKa = 4.64QRR284 pKa = 11.84TFWLSNSVEE293 pKa = 4.21ILNNTQSIMVAVEE306 pKa = 3.95TEE308 pKa = 4.05LAIEE312 pKa = 4.01DD313 pKa = 4.59AIYY316 pKa = 10.23GVDD319 pKa = 3.83MVWGNYY325 pKa = 8.96YY326 pKa = 8.44PTGSNSSTSISGSGSGSSSNSTAPSGSGSNSTGLSGSGTNSTVGVNGTTSSNSTSNANGGSSCGAAPSSTIDD398 pKa = 3.41GFPAVACGVSDD409 pKa = 5.89FDD411 pKa = 3.99TQLDD415 pKa = 4.05DD416 pKa = 3.48VLGYY420 pKa = 11.0LDD422 pKa = 5.8FSSTDD427 pKa = 3.23YY428 pKa = 11.22DD429 pKa = 3.81ASLEE433 pKa = 4.09DD434 pKa = 4.27FSPDD438 pKa = 3.03VSFDD442 pKa = 4.5AEE444 pKa = 5.08DD445 pKa = 5.4LDD447 pKa = 6.5DD448 pKa = 6.62DD449 pKa = 4.61DD450 pKa = 4.7ATMEE454 pKa = 3.98RR455 pKa = 11.84RR456 pKa = 11.84ALLGRR461 pKa = 11.84SHH463 pKa = 6.53LRR465 pKa = 11.84KK466 pKa = 9.87RR467 pKa = 11.84GIFSALLSVGEE478 pKa = 4.1QLSSAVVAVATTGVALARR496 pKa = 11.84AAVQVIPGVSNFIASATEE514 pKa = 3.69FDD516 pKa = 4.2PAVHH520 pKa = 6.04GTATLALAPDD530 pKa = 4.85DD531 pKa = 4.54SATSPWGAAAQLYY544 pKa = 7.79TSSSASATTAADD556 pKa = 2.96ITLYY560 pKa = 10.68CVNCGLKK567 pKa = 10.59GHH569 pKa = 5.7VTLSGSAKK577 pKa = 10.2FNVIDD582 pKa = 4.36GLHH585 pKa = 6.18GLSTSINADD594 pKa = 3.36LEE596 pKa = 4.61AGLNLGLVAQATFKK610 pKa = 10.04DD611 pKa = 4.03TKK613 pKa = 9.51TKK615 pKa = 11.0SLIKK619 pKa = 10.26QAIPDD624 pKa = 3.8LGVSVKK630 pKa = 10.5GVFAAGVYY638 pKa = 9.81IAVDD642 pKa = 3.78AVATLDD648 pKa = 4.89IEE650 pKa = 4.56AQGQALIGITMTISDD665 pKa = 4.37FQATLNLLDD674 pKa = 4.44EE675 pKa = 4.74NSAASSSISGYY686 pKa = 8.91TPTFKK691 pKa = 9.95KK692 pKa = 9.22TFEE695 pKa = 4.11ASGQISASATLALPVSLNVGIEE717 pKa = 3.97ITPLSYY723 pKa = 11.0KK724 pKa = 9.36KK725 pKa = 8.74TLSLIEE731 pKa = 4.01QPSLYY736 pKa = 10.69GNVSFAGSAGGADD749 pKa = 3.58AASDD753 pKa = 3.53TCNNGFQYY761 pKa = 10.2FASAQNDD768 pKa = 3.21VSFDD772 pKa = 3.39FFGIKK777 pKa = 9.28TFTLNHH783 pKa = 6.4YY784 pKa = 9.13DD785 pKa = 4.06SPPIQQGCKK794 pKa = 10.34LLDD797 pKa = 4.39DD798 pKa = 4.64SSSTSSSTPAASSGTSSAVTEE819 pKa = 4.68TPSSTGDD826 pKa = 3.18AAADD830 pKa = 3.86SNTATSSGTDD840 pKa = 3.47STSTGAPSPTDD851 pKa = 3.36DD852 pKa = 4.38AAATSTTAGSGTDD865 pKa = 3.25VPTNTGPTSTTDD877 pKa = 3.15TDD879 pKa = 4.09PAPTPSNPTTGDD891 pKa = 3.44STSSKK896 pKa = 10.39SKK898 pKa = 10.66RR899 pKa = 11.84QITDD903 pKa = 2.6SGTVIDD909 pKa = 6.06DD910 pKa = 4.16DD911 pKa = 6.46DD912 pKa = 5.29EE913 pKa = 4.48DD914 pKa = 4.47TFASDD919 pKa = 5.9DD920 pKa = 3.77GDD922 pKa = 4.01EE923 pKa = 4.5TDD925 pKa = 3.95STDD928 pKa = 5.02DD929 pKa = 4.01PVDD932 pKa = 3.96AQDD935 pKa = 5.2SSTDD939 pKa = 3.64PDD941 pKa = 3.51NAEE944 pKa = 4.03YY945 pKa = 9.23TQDD948 pKa = 3.68SSAALNATDD957 pKa = 4.29NAGSDD962 pKa = 3.34GGLTFVTINEE972 pKa = 3.91IHH974 pKa = 6.61NAFQLVSDD982 pKa = 4.29EE983 pKa = 4.42NGNLYY988 pKa = 10.66AGAPSTGDD996 pKa = 3.48DD997 pKa = 4.03DD998 pKa = 4.65GASLFAVADD1007 pKa = 3.96TVVVGNDD1014 pKa = 2.82GDD1016 pKa = 4.51MILHH1020 pKa = 6.58YY1021 pKa = 10.73YY1022 pKa = 9.09PDD1024 pKa = 3.49VMDD1027 pKa = 5.36AYY1029 pKa = 8.58NTSRR1033 pKa = 11.84IRR1035 pKa = 11.84MSYY1038 pKa = 9.89EE1039 pKa = 3.24DD1040 pKa = 5.42HH1041 pKa = 6.54IPKK1044 pKa = 10.43GSEE1047 pKa = 3.87LVTLMALDD1055 pKa = 4.25YY1056 pKa = 11.44DD1057 pKa = 4.56SDD1059 pKa = 4.1DD1060 pKa = 4.3SSNQNILFAVDD1071 pKa = 3.32TKK1073 pKa = 11.59GNAFSLVLCDD1083 pKa = 4.86FSNGADD1089 pKa = 3.03SKK1091 pKa = 11.44LFIVRR1096 pKa = 11.84DD1097 pKa = 3.74TTGVEE1102 pKa = 3.85NLTLEE1107 pKa = 4.21KK1108 pKa = 10.47LRR1110 pKa = 11.84YY1111 pKa = 8.23ICTGAPVSQCDD1122 pKa = 3.4AVALVLGATSTNSS1135 pKa = 2.96

Molecular weight: 115.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D1YVL4|A0A0D1YVL4_9EURO Isoform of A0A0D1XEV3 Uncharacterized protein OS=Exophiala sideris OX=1016849 GN=PV11_02182 PE=4 SV=1
MM1 pKa = 7.67SILGSRR7 pKa = 11.84SGILWLRR14 pKa = 11.84SARR17 pKa = 11.84PFVLSICAVFSSSLSEE33 pKa = 3.82HH34 pKa = 6.03LRR36 pKa = 11.84YY37 pKa = 9.6HH38 pKa = 6.46LSCRR42 pKa = 11.84GTTFWQQGRR51 pKa = 11.84VRR53 pKa = 11.84LPPRR57 pKa = 11.84TYY59 pKa = 10.42KK60 pKa = 10.43ISQTYY65 pKa = 10.17ILTSSTGCLKK75 pKa = 10.67PSTILSFGLPFSTKK89 pKa = 9.02PWGCLRR95 pKa = 11.84RR96 pKa = 11.84CRR98 pKa = 11.84HH99 pKa = 5.46NVPIPSLRR107 pKa = 11.84WTRR110 pKa = 11.84KK111 pKa = 8.9VCSLNHH117 pKa = 6.21KK118 pKa = 8.26EE119 pKa = 4.14KK120 pKa = 10.59KK121 pKa = 9.92EE122 pKa = 3.87KK123 pKa = 10.37FSEE126 pKa = 3.97VGEE129 pKa = 4.47NYY131 pKa = 9.61GQPAHH136 pKa = 6.42VPEE139 pKa = 4.35SSPRR143 pKa = 11.84DD144 pKa = 3.44RR145 pKa = 11.84HH146 pKa = 5.04QVSSLDD152 pKa = 3.47VTMGASISRR161 pKa = 11.84PSVHH165 pKa = 6.3SLVVGAHH172 pKa = 6.22SADD175 pKa = 3.07GMYY178 pKa = 10.27QIIDD182 pKa = 3.67GCRR185 pKa = 11.84PNASVYY191 pKa = 9.84PPPGHH196 pKa = 6.83RR197 pKa = 11.84RR198 pKa = 11.84RR199 pKa = 11.84ASRR202 pKa = 11.84TSRR205 pKa = 11.84PSSEE209 pKa = 4.27SASLLL214 pKa = 3.74

Molecular weight: 23.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10191

650

10841

5408673

50

5532

498.9

55.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.584 ± 0.019

1.183 ± 0.01

5.81 ± 0.017

6.121 ± 0.022

3.642 ± 0.012

6.728 ± 0.021

2.447 ± 0.011

4.828 ± 0.015

4.895 ± 0.021

8.804 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.226 ± 0.009

3.665 ± 0.013

5.991 ± 0.021

4.235 ± 0.014

6.061 ± 0.021

8.263 ± 0.026

6.229 ± 0.022

6.111 ± 0.014

1.421 ± 0.008

2.755 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski