Haladaptatus sp. R4

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Haladaptatus; unclassified Haladaptatus

Average proteome isoelectric point is 5.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4086 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A161ZLQ8|A0A161ZLQ8_9EURY Uncharacterized protein OS=Haladaptatus sp. R4 OX=1679489 GN=A4G99_08145 PE=4 SV=1
MM1 pKa = 7.42EE2 pKa = 4.61LTWYY6 pKa = 9.84GHH8 pKa = 5.25STWHH12 pKa = 5.08VTVDD16 pKa = 3.58DD17 pKa = 4.41TEE19 pKa = 4.82LLIDD23 pKa = 4.52PFFDD27 pKa = 4.08NPKK30 pKa = 9.47TDD32 pKa = 3.62TDD34 pKa = 4.16PEE36 pKa = 4.26EE37 pKa = 4.79LDD39 pKa = 3.35PDD41 pKa = 4.32YY42 pKa = 11.83VLLTHH47 pKa = 6.39GHH49 pKa = 7.03ADD51 pKa = 4.02HH52 pKa = 7.1IADD55 pKa = 3.85ADD57 pKa = 4.12RR58 pKa = 11.84FRR60 pKa = 11.84DD61 pKa = 3.42AHH63 pKa = 6.23FVATPEE69 pKa = 4.18VIGYY73 pKa = 7.76VTDD76 pKa = 3.7EE77 pKa = 4.44YY78 pKa = 11.39GVEE81 pKa = 4.84DD82 pKa = 3.85GTGMNMGGTFEE93 pKa = 6.02ADD95 pKa = 3.22DD96 pKa = 4.5AFVTMVRR103 pKa = 11.84ADD105 pKa = 3.59HH106 pKa = 6.68TNGLDD111 pKa = 3.18TGYY114 pKa = 10.14GASGGMPAGFVISDD128 pKa = 3.82TKK130 pKa = 9.11PTQVSDD136 pKa = 3.76EE137 pKa = 4.19EE138 pKa = 4.74SEE140 pKa = 4.51SFYY143 pKa = 11.07HH144 pKa = 7.49AGDD147 pKa = 3.58TSLMTEE153 pKa = 3.88MRR155 pKa = 11.84DD156 pKa = 3.52VIGPFLEE163 pKa = 4.71PDD165 pKa = 3.34AVAVPIGDD173 pKa = 4.49HH174 pKa = 5.35YY175 pKa = 10.22TMGPWQAAVAVDD187 pKa = 3.85WVDD190 pKa = 2.96ADD192 pKa = 3.99YY193 pKa = 11.19AFPMHH198 pKa = 7.11YY199 pKa = 8.87DD200 pKa = 3.69TFPPIEE206 pKa = 4.24QDD208 pKa = 3.04PEE210 pKa = 4.26DD211 pKa = 3.64FVRR214 pKa = 11.84EE215 pKa = 4.07VEE217 pKa = 4.23ATGNDD222 pKa = 3.68AEE224 pKa = 4.43VHH226 pKa = 5.77VLDD229 pKa = 5.06GDD231 pKa = 3.96EE232 pKa = 4.53TFDD235 pKa = 6.31LSDD238 pKa = 3.63QLWW241 pKa = 3.42

Molecular weight: 26.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166RVH1|A0A166RVH1_9EURY Uncharacterized protein OS=Haladaptatus sp. R4 OX=1679489 GN=A4G99_15645 PE=4 SV=1
MM1 pKa = 7.68TDD3 pKa = 2.84GLVRR7 pKa = 11.84RR8 pKa = 11.84TYY10 pKa = 10.68DD11 pKa = 3.0EE12 pKa = 4.12RR13 pKa = 11.84EE14 pKa = 3.61RR15 pKa = 11.84LLRR18 pKa = 11.84TKK20 pKa = 9.92SVKK23 pKa = 10.36RR24 pKa = 11.84PTRR27 pKa = 11.84DD28 pKa = 3.61AQWKK32 pKa = 7.23CQSRR36 pKa = 11.84GVQTDD41 pKa = 3.23YY42 pKa = 11.89DD43 pKa = 3.97EE44 pKa = 6.4DD45 pKa = 3.94FVLRR49 pKa = 11.84NGMEE53 pKa = 4.17SEE55 pKa = 4.12RR56 pKa = 11.84TEE58 pKa = 3.5DD59 pKa = 2.75WGRR62 pKa = 11.84FTGPRR67 pKa = 11.84VSNGSSRR74 pKa = 11.84LRR76 pKa = 11.84SHH78 pKa = 6.43GRR80 pKa = 11.84KK81 pKa = 8.99CSLRR85 pKa = 11.84KK86 pKa = 9.36RR87 pKa = 11.84VSLL90 pKa = 4.07

Molecular weight: 10.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4086

0

4086

1085222

44

2107

265.6

29.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.185 ± 0.043

0.73 ± 0.012

7.706 ± 0.045

8.098 ± 0.062

3.732 ± 0.027

8.335 ± 0.036

2.144 ± 0.02

4.792 ± 0.032

2.576 ± 0.028

8.942 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.019 ± 0.018

2.909 ± 0.024

4.447 ± 0.025

2.57 ± 0.025

6.291 ± 0.045

6.182 ± 0.032

6.618 ± 0.04

8.58 ± 0.042

1.247 ± 0.017

2.896 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski